Search

Your search keyword '"Bo Zhang"' showing total 4 results
Start Over Author "Bo Zhang" Topic fusarium solani
4 results on '"Bo Zhang"'

1. Study on signal transmission mechanism of arbuscular mycorrhizal hyphal network against root rot of Salvia miltiorrhiza.

Author

Han, Song, Na, Li, Rongchao, Zhang, Xiuqin, Hu, Wenyu, Zhang, Bo, Zhang, Xinpeng, Li, Zhen, Wang, and Jie, Xin

Subjects
SALVIA miltiorrhiza, ROOT rots, FUSARIUM solani, SALICYLIC acid, JASMONIC acid, GENE expression, PATHOGENIC fungi
Abstract

To explore the signal transmission mechanism of the arbuscular mycorrhizal network against root rot of Salvia miltiorrhiza. In this experiment, the arbuscular mycorrhizal hyphal network was established among Salvia miltiorrhiza plants, and a two plant three-compartment culture model was established. The root of the donor Salvia miltiorrhiza was inoculated with the pathogenic fungi Fusarium solani. The changes of hormone signals such as jasmonic acid and salicylic acid and the expression of related defense genes in the recipient Salvia miltiorrhiza plants in different periods were measured, to study the underground disease resistance signal transmission mechanism among medicinal plants. Salvia miltiorrhiza can transmit the signal of resistance to root rot through the jasmonic acid pathway; When plants suffer from disease stress, the content of JA increases significantly, and the increase of JA content will inhibit the content of SA in plants; The gene expression of PR-10 gene in the roots of Salvia miltiorrhiza with arbuscular mycorrhizal network infected by pathogenic fungi was 17.56 times higher than that inoculated only with pathogenic fungi; Changes in hormone content will also cause changes in the expression of related defense genes, such as SnRK2 is inhibited by ABA in the signal transduction pathway, while JA and ABA show antagonistic changes after inoculation of pathogenic fungi in Salvia miltiorrhiza, so JA may positively regulate the expression of SnRK2 gene. Plants can transmit signals through AM hyphal network after being stressed by the pathogen Fusarium solani. In the arbuscular mycorrhizal hyphal network, JA has important significance for the signal transmission of resistance to root rot and disease resistance of Salvia miltiorrhiza, which can make Salvia miltiorrhiza ready for stress resistance and improve the stress resistance of Salvia miltiorrhiza. This experiment is of great significance to further analyze the signal transmission mechanism of the arbuscular mycorrhizal hyphal network. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

2. Knock down of chitosanase expression in phytopathogenic fungus Fusarium solani and its effect on pathogenicity

Author

Xiaoming Bao, Huaiwei Liu, Bo Zhang, and Changsong Li

Subjects
Chitosanase activity, Glycoside Hydrolases, biology, Virulence, General Medicine, biology.organism_classification, Microbiology, Small hairpin RNA, RNA silencing, Fusarium, Gene Expression Regulation, Fungal, Gene Knockdown Techniques, Gene expression, Genetics, Chitosanase, Fusarium solani, Mycelium
Abstract

Chitosanases are lytic enzymes involved in the degradation of chitosan, a component of fungal cell walls. The phytopathogenic fungus Fusarium solani produces an extracellular chitosanase, CSN1, the role of which in the physiology and virulence of the fungus remains to be expounded. Here, we studied the expression of the CSN1 gene through gene silencing and examined its effect on fungal pathogenicity. A vector construct encoding a hairpin RNA (hpRNA) of CSN1 was constructed and introduced into the F. solani 0114 strain. The results revealed that majority of the transformants exhibited a significant reduction in chitosanase activity compared with the wild-type strain. Further, transformants with silenced CSN1 exhibited no change in mycelial growth and spore formation. However, pea pod and seedling bioassays indicated that transformants with silenced CSN1 were more virulent compared with the wild-type strain, and in sharp contrast to strains in which overexpression of the CSN1 gene resulted in virulence reduction. Although the mechanism remains unclear, our findings did suggest that F. solani chitosanase has a negative effect on fungal pathogenicity.

Published
2010

3. Pathogenic spectrum of fungal keratitis and specific identification of Fusarium solani

Author

Yanqiu Yang, Yunfeng Zhang, Koji Yokoyama, Bo Zhang, Wengang Song, Dan He, Jilong Hao, and Li Wang

Subjects
Fusarium, Adult, Male, Rural Population, China, Polymerase Chain Reaction, Microbiology, Multiplex polymerase chain reaction, medicine, Humans, Fungal keratitis, Corneal Ulcer, DNA, Fungal, Mycological Typing Techniques, DNA Primers, Retrospective Studies, Electrophoresis, Agar Gel, Aspergillus, biology, food and beverages, Nucleic acid amplification technique, Eye infection, Middle Aged, biology.organism_classification, medicine.disease, Mycoses, Female, Fusarium solani, Eye Infections, Fungal, Nucleic Acid Amplification Techniques, Specific identification
Abstract

Purpose To investigate the predominant causative pathogens and epidemiologic features of fungal keratitis and establish a rapid, specific molecular method to detect fungal keratitis caused by Fusarium solani. Methods A total of 174 patients with presumed fungal keratitis and 174 affected eyes were examined. Isolates from corneal specimens were identified according to morphologic and physiological characteristics. The primers that were designed for F. solani were tested to confirm whether they had species specificity. Multiplex PCR with universal fungal and F. solani-specific primers was performed with fungal and bacterial strains and was used to detect microorganisms in the clinical specimens. Results A total of 160 patients (92.0%) were diagnosed with fungal infection by either potassium hydroxide wet-mount or microbiologic culture. Fungal cultures were positive in 128 patients (73.6%) with 139 fungal isolates. Fusarium (48.2%) was the most frequently isolated genus, in which F. solani (35.2%) was the most common species, followed by the Aspergillus (18.7%) and Candida (16.6%) genera. The PCR results showed that the designed primers were species specific and suitable for specific identification of F. solani. The multiplex PCR of 3-day broth cultures could identify and distinguish F. solani from other pathogens rapidly and specifically from clinical specimens. Conclusions Fusarium species, especially F. solani, were found to be the predominant cause of fungal keratitis in northeast China. The established multiplex PCR method could have potential advantages for rapid detection of F. solani. These findings might have significance for early diagnosis and treatment of fungal keratitis.

Published
2011

4. Knock down of chitosanase expression in phytopathogenic fungus Fusarium solani and its effect on pathogenicity.

Author

Huaiwei Liu, Bo Zhang, Changsong Li, and Xiaoming Bao

Subjects
*PATHOGENIC fungi, *FUSARIUM oxysporum, *FUNGAL diseases of plants, *PHYTOPATHOGENIC fungi, *NUCLEIC acids
Abstract

Chitosanases are lytic enzymes involved in the degradation of chitosan, a component of fungal cell walls. The phytopathogenic fungus Fusarium solani produces an extracellular chitosanase, CSN1, the role of which in the physiology and virulence of the fungus remains to be expounded. Here, we studied the expression of the CSN1 gene through gene silencing and examined its effect on fungal pathogenicity. A vector construct encoding a hairpin RNA (hpRNA) of CSN1 was constructed and introduced into the F. solani 0114 strain. The results revealed that majority of the transformants exhibited a significant reduction in chitosanase activity compared with the wild-type strain. Further, transformants with silenced CSN1 exhibited no change in mycelial growth and spore formation. However, pea pod and seedling bioassays indicated that transformants with silenced CSN1 were more virulent compared with the wild-type strain, and in sharp contrast to strains in which overexpression of the CSN1 gene resulted in virulence reduction. Although the mechanism remains unclear, our findings did suggest that F. solani chitosanase has a negative effect on fungal pathogenicity. [ABSTRACT FROM AUTHOR]

Published
2010
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results