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Your search keyword '"Gpr97"' showing total 2 results
Start Over Descriptor "Gpr97" Topic g-protein-coupled receptor
2 results on '"Gpr97"'

1. Signaling property study of adhesion G-protein-coupled receptors

Author

Gupte, Jamila, Swaminath, Gayathri, Danao, Jay, Tian, Hui, Li, Yang, and Wu, Xinle

Subjects
*CELLULAR signal transduction, *CELL adhesion, *G protein coupled receptors, *CELL lines, *INOSITOL phosphates, *BIOLOGICAL assay, *CELL physiology
Abstract

Abstract: Adhesion G-protein-coupled receptors (GPCR) are special members of GPCRs with long N-termini containing multiple domains. We overexpressed our collection of receptors together with G-proteins in mammalian cell lines and measured the concentrations of intracellular signaling molecules, such as inositol phosphate and cAMP. Our results show that a subset of tested adhesion GPCRs has constitutive activities and is capable of coupling to a variety of G-proteins. In addition, we have identified a small molecule compound that specifically activates one of the subfamily members, GPR97, and the activation was confirmed by an independent GTPγS assay. These findings suggest classical GPCR screening assays could be applied to de-orphanize these receptors, and provide pharmacological tools to improve understanding of the physiological functions of these receptors. [Copyright &y& Elsevier]

Published
2012
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2. Signaling property study of adhesion G-protein-coupled receptors

Author

Gayathri Swaminath, Xinle Wu, Jay Danao, Jamila Gupte, Hui Tian, and Yang Li

Subjects
Cell signaling, GPR97, G-protein-coupled receptor, Inositol Phosphates, Biophysics, Class C GPCR, CHO Cells, Biology, Transfection, Biochemistry, Rhodopsin-like receptors, Cell Line, Receptors, G-Protein-Coupled, Structural Biology, Cricetinae, Cyclic AMP, Genetics, Animals, Humans, Inositol phosphate, Receptor, Molecular Biology, G protein-coupled receptor, chemistry.chemical_classification, Cell Biology, Small molecule, Cell biology, chemistry, Guanosine 5'-O-(3-Thiotriphosphate), Adhesion, Signal transduction, Signal Transduction
Abstract

Adhesion G-protein-coupled receptors (GPCR) are special members of GPCRs with long N-termini containing multiple domains. We overexpressed our collection of receptors together with G-proteins in mammalian cell lines and measured the concentrations of intracellular signaling molecules, such as inositol phosphate and cAMP. Our results show that a subset of tested adhesion GPCRs has constitutive activities and is capable of coupling to a variety of G-proteins. In addition, we have identified a small molecule compound that specifically activates one of the subfamily members, GPR97, and the activation was confirmed by an independent GTPγS assay. These findings suggest classical GPCR screening assays could be applied to de-orphanize these receptors, and provide pharmacological tools to improve understanding of the physiological functions of these receptors.

Published
2012
Full Text
View/download PDF

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