1. A Gas Chromatography–Mass Spectrometry Method for Toxicological Analysis of MDA, MDEA and MDMA in Vitreous Humor Samples from Victims of Car Accidents.
- Author
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Ishikawa, Aline Akemi, Bordin, Dayanne Mozaner, Campos, Eduardo Geraldo de, Martinis, Bruno Spinosa De, Blanes, Lucas, and Doble, Philip
- Subjects
PSYCHIATRIC drugs ,DRUG analysis ,VITREOUS humor ,GAS chromatography/Mass spectrometry (GC-MS) ,TOXICOLOGY ,ACCIDENT victims - Abstract
Vitreous humor (VH) shows excellent potential as a matrix of choice for postmortem analytical toxicology due to the ease of sampling and low metabolic activity. This study demonstrates a simple and rapid analytical method to identify and quantify 3,4-methylenedioxyamphetamine, 3,4-methylenedioxymethamphetamine and 3,4-methylenedioxy-ethylamphetamine in VH. Samples were collected with a simple eye puncture procedure, followed by liquid–liquid extraction and derivatization with heptafluorobutyric anhydride and analysis via gas chromatography–mass spectrometry. The accuracy of the method ranged 97–103%, intra-assay precision was between 4.54 and 9.14% relative standard deviation (RSD) and interassay precision ranged from 6.92 to 10.59% RSD. Limits of detection and quantification ranged from 1.0 to 2.5 ng/mL and 10 ng/mL, respectively. The validated method was successfully applied to detect methylenedioxyamphetamine derivatives in VH samples collected from victims of fatal car crashes. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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