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Abstract: A gastric cancer cell line, AGS has high-level expression of CD40. CD40 is present on the surfaces of a large variety of cells, including B cells, endothelial cells, dendritic cells and some carcinoma cells, and delivers signals regulating diverse cellular responses, such as proliferation, differentiation, growth suppression, cell death. In this research, the expression of CD40 and CD40 transcription in gastric cancer cell lines (AGS, BGC-823, HGC-27, SGC-7901) was investigated by semi-quantitative RT-PCR. Cancer cell proliferation inhibitory assay was also performed using different concentrations of anti-CD40 monoclonal antibody (mAb), 5C11. Results indicated that treatment of 5C11 alone and combined 5C11, IFN-α and 5-FU both significantly inhibited cancer cell proliferation. The synergistic effects of combined 5C11, IFN-α and 5-FU on growth inhibition of human gastric cancer AGS cells could be observed. These results suggest that humanized anti-CD40 monoclonal antibody (mAb), 5C11 can be used as a valuable reagent for clinical application. [Copyright &y& Elsevier]

Abstract: One major polysaccharide fractions, glucose, were isolated from the polysaccharides extract of Astragalus (AP), a valuable traditional Chinese medicine, using thin-layer chromatography (TLC) and Sephadex G-100 chromatography. HPLC and IR methods were used for a qualitative and quantitative determination of from polysaccharides of Astragalus. The HPLC method was validated for linearity, precision and accuracy. The results indicated that polysaccharides of Astragalus is an α-(1→4)-d-glucan with α-(1→6)-linked branches attached to the O-6 of branch points. Bioactivity tests showed that polysaccharides of Astragalus is active for spleen lymphocytes proliferation. The polysaccharides also presented anti-inflammatory activities. These data together suggest that polysaccharides of Astragalus presents significant immune modulating activity, thus supporting the popular use of the polysaccharides in the treatment of gastric cancer diseases. [Copyright &y& Elsevier]