1. Interleukin-17C in Human Helicobacter pylori Gastritis.
- Author
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Tanaka S, Nagashima H, Cruz M, Uchida T, Uotani T, Jiménez Abreu JA, Mahachai V, Vilaichone RK, Ratanachu-Ek T, Tshering L, Graham DY, and Yamaoka Y
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Bacterial Proteins genetics, Bhutan, Cell Line, Dominican Republic, Female, Gastric Mucosa metabolism, Gastric Mucosa microbiology, Gastric Mucosa pathology, Gastritis microbiology, Gastritis physiopathology, Gene Regulatory Networks, Genomic Islands, Genotype, Helicobacter Infections epidemiology, Helicobacter Infections microbiology, Helicobacter pylori genetics, Humans, Male, Middle Aged, RNA, Messenger genetics, Real-Time Polymerase Chain Reaction, Up-Regulation, Young Adult, Gastric Mucosa immunology, Gastritis immunology, Helicobacter Infections immunology, Helicobacter pylori immunology, Interleukin-17 genetics, Interleukin-17 immunology
- Abstract
The interleukin-17 (IL-17) family of cytokines (IL-17A to IL-17F) is involved in many inflammatory diseases. Although IL-17A is recognized as being involved in the pathophysiology of Helicobacter pylori -associated diseases, the role of other IL-17 cytokine family members remains unclear. Microarray analysis of IL-17 family cytokines was performed in H. pylori -infected and uninfected gastric biopsy specimens. IL-17C mRNA was upregulated approximately 4.5-fold in H. pylori -infected gastric biopsy specimens. This was confirmed by quantitative reverse transcriptase PCR in infected and uninfected gastric mucosa obtained from Bhutan and from the Dominican Republic. Immunohistochemical analysis showed that IL-17C expression in H. pylori -infected gastric biopsy specimens was predominantly localized to epithelial and chromogranin A-positive endocrine cells. IL-17C mRNA levels were also significantly greater among cagA -positive than cagA -negative H. pylori infections ( P = 0.012). In vitro studies confirmed an increase in IL-17C mRNA and protein levels in cells infected with cagA -positive infections compared to cells infected with either cagA -negative or cag pathogenicity island (PAI) mutant. Chemical inhibition of IκB kinase (IKK), mitogen-activated protein extracellular signal-regulated kinase (MEK), and Jun N-terminal kinase (JNK) inhibited induction of IL-17C proteins in infected cells, whereas p38 inhibition had no effect on IL-17C protein secretion. In conclusion, H. pylori infection was associated with a significant increase in IL-17C expression in human gastric mucosa. The role of IL-17C in the pathogenesis of H. pylori -induced diseases remains to be determined., (Copyright © 2017 American Society for Microbiology.)
- Published
- 2017
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