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The complete mitochondrial DNA genome of Anabarilius brevianalis was determined. The entire mitochondrial genome is 16,610 bp in length and contains 13 protein-coding genes, 2 ribosomal RNA (rRNA) ...

The complete mitochondrial DNA genome of Anabarilius grahami was determined by the primer walking sequence method. The entire mitochondrial genome is 16,612 bp in length and contains 13 protein-coding genes, 2 ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA) genes, and one control region (CR). As in other teleosts, most mitochondrial genes are encoded on the heavy strand, except for ND6 and eight tRNA genes which are encoded on the light strand. The overall nucleotide composition was 28.9% A, 24.8% T, 27.9% C, and 18.3% G, respectively, with a slight A+T bias (53.7%). Phylogenetic analysis based on 12 concatenated protein-coding genes of the heavy strand shows the evolutionary position of A. grahami is more closely related to two species of the genus Hemiculter within the subfamily Cultrinae.

Hemiculterella sauvagei, a Chinese minnow endemic to the upper Yangtze River, is an important part of the aquatic ecosystem and plays a vital ecological role in maintaining aquatic biodiversity. In this study, we provide the first mitochondrial genome of H. sauvagei. The complete mitochondrial genome of H. sauvagei is 16618 bp in length, containing 13 protein-coding genes, two rRNA genes (12 S and 16 S rRNA), 22 tRNA genes and a non-coding control region (D-loop). The gene nucleotide composition of H. sauvagei is A = 29.9%, T = 27.0%, C = 17.4% and G = 25.6%, with a slight AT bias of 56.9%. The complete mitochondrial genome of H. sauvagei would be useful for further studies on genetic diversity and molecular phylogenetic relationship of the subfamily Cultrinae.

Toxabramis houdemeri belongs to the genus Toxabramis in the subfamily of Cultrinae (Cyprinidae). We first determine the complete mitochondrial genome of T. houdemeri in this study. It is 16 618 bp in length, with the base composition on the heavy strand: 30.79% A, 16.61% G, 27.29% C, and 25.31% T. It has the typical vertebrate mitochondrial gene arrangement, including 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and a D-loop region. Phylogenetic analysis showed that T. houdemeri was clustered into one branch of the subfamily of Cultrinae, and closely related to Hemiculter leucisculus. The present study will contribute to genetic resources conservation of T. houdemeri and studying its population genetic structure and phylogenetic relationships.

The complete mitogenome was first determined for a minnow Pseudohemiculter dispar in this study. It is 16,621 bp in length, including 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and 1 control region. Overall nucleotide base composition is 30.30% A, 27.10% C, 17.10% G, and 25.50% T, with a A+T bias. Except for the tRNA-Ser2 that lost the dihydrouridine arm and replaced with one 11-bp loop, the other 22 tRNA genes can fold into the typical cloverleaf secondary structures. The origin of light-strand replication (OL) was found between the tRNA-Asn and tRNA-Cys genes, while the termination-associated sequence (TAS) was found in the control region. Both OL and TAS can form the hairpin structure that acts as the signals participated in the mitochondrial DNA replication.

The complete mitochondrial genome of Osteobrama belangeri was obtained, using Illumina high-throughput NextSeq 500 with 2 × 150 bp sequencing of mitochondrial DNA. The mitochondrial genome of O. belangeri was 16,602 bp in length (GenBank Accession No. KY887473), comprised of 13 protein coding genes, 22 tRNA genes, 2 rRNA genes and a control region, i.e. D-loop. In present mitogenome, 11 short sequence repeats were identified and validated in silico. The arrangement of genes was found identical to other Cypriniformes fish mitogenomes, available in NCBI database. Phylogenetic relationship established in the present study also supported that genus Osteobrama is member of subfamily Cyprininae (tribe: smiliogastrini) not Cultrinae, which provide useful insights into taxonomic status of the genus.

The complete mitogenome was first determined for a minnow Hemiculter leucisculus in this study. It is 16,622 bp in length and has 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and 1 control region. Overall nucleotide base composition is 30.4% A, 27.10% C, 17.10% G and 25.40% T. All the tRNA genes can fold into the typical clover-leaf secondary structures, except the tRNA-Ser2 which loses the dihydrouridine arm and is replaced with one 11 bp loop. The origin of light-strand replication (OL) was found between the tRNA-Asn and tRNA-Cys genes while the termination associated sequence (TAS) and the conserved sequence blocks (CSB1-3) were found in the control region.

The complete mitochondrial genome of Sinibrama macrops was first determined in this study. The genome is 16,626 bp in length, including 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and 1 control region. Its gene arrangement and translation direction were identical to those of other typical vertebrates. The overall base composition is 30.7% A, 27.9% C, 16.7% G and 24.7% T, with the negative GC skew value ( - 0.251). In a long term, S. macrops was confused with Sinibrama wui, which was confirmed as an invalid species by recent morphological study. Therefore, the mitochondrial genome of S. macrops can contribute to the molecular identification of the species and further phylogenetic study of Cyprinidae.

In this study, the complete mitochondrial DNA genome sequence of Sinibrama taeniatus was first determined by DNA sequencing based on the PCR fragments. The complete mitochondrial DNA (mtDNA) genome sequence of S. taeniatus was a circular molecule of 16,623 bp in length. It consisted of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and a control region (D-loop). The gene nucleotide composition of S. taeniatus was 31.3% A, 26.8% C, 16.1% G, and 25.8% T, with a relatively high A + T content (57.1%). The results could provide useful data for studying genetic diversity and phylogenetics in S. taeniatus and related species.

The complete mitochondrial DNA (mtDNA) sequence of Megalobrama skolkovii was first presented in this study. The mitochondrial genome is 16,620 bp in length, including 13 protein-coding genes, 2 ribosomal RNA gens (12S rRNA and 16S rRNA), 22 transfer RNA genes (tRNA) and a control region (D-loop), with the gene identical to that of typical vertebrates. The overall base composition of the light strand are 31.23% A, 24.73% T, 16.16% G and 27.88% C. Two copies of tandem repeat sequence was found in the control region.

The complete mitogenome of Pseudolaubuca engraulis was determined using long-distance polymerase chain reaction and sequenced by primer walking method. It is 16,612 bp in length and contains 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA (tRNA) genes and 1 A + T-rich control region. The gene arrangement and characterization of P. engraulis are similar to most vertebrates. The overall base composition of the heavy strand is 27.1% A, 27.5% C, 25.9% T and 19.5% G, with a slight A + T bias. The shortest tRNA-Ser2 lacks the dihydrouridine arm and is replaced with a simple loop. The termination-associated sequence and the conserved sequence blocks (1–3) are found in the control region.

Culter dabryi, a medium-sized economic cyprinid fish, is widely distributed in East Asia. We sequenced the complete mitochondrial genome of this species. The genome is 16,622 bp in length, and consists of 22 transfer RNA genes, 13 protein-coding genes, two ribosomal RNA genes and the non-coding control region with a structural organization conserved relative to that of other fishes. The overall base composition of C. dabryi in descending order is A 31.51%, C 27.60%, T 24.95% and G 15.94%, with a slight A + T bias. The mitogenome sequence data may provide useful information to the population genetics analysis of C. dabryi and the elucidation of evolutionary mechanisms in the endemic clade of East Asian Cyprinidae.