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4 results on '"TALE nucleases"'

1. Recent Advances in Tomato Gene Editing.

Author

Larriba E, Yaroshko O, and Pérez-Pérez JM

Subjects
Genome, Plant, CRISPR-Cas Systems, Crops, Agricultural genetics, Plant Breeding, Gene Editing methods, Solanum lycopersicum
Abstract

The use of gene-editing tools, such as zinc finger nucleases, TALEN, and CRISPR/Cas, allows for the modification of physiological, morphological, and other characteristics in a wide range of crops to mitigate the negative effects of stress caused by anthropogenic climate change or biotic stresses. Importantly, these tools have the potential to improve crop resilience and increase yields in response to challenging environmental conditions. This review provides an overview of gene-editing techniques used in plants, focusing on the cultivated tomatoes. Several dozen genes that have been successfully edited with the CRISPR/Cas system were selected for inclusion to illustrate the possibilities of this technology in improving fruit yield and quality, tolerance to pathogens, or responses to drought and soil salinity, among other factors. Examples are also given of how the domestication of wild species can be accelerated using CRISPR/Cas to generate new crops that are better adapted to the new climatic situation or suited to use in indoor agriculture.

Published
2024
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2. Gene targeting in rats using transcription activator-like effector nucleases.

Author

Ménoret, Séverine, Tesson, Laurent, Rémy, Séverine, Usal, Claire, Thépenier, Virginie, Thinard, Reynald, Ouisse, Laure-Hélène, De Cian, Anne, Giovannangeli, Carine, Concordet, Jean-Paul, and Anegon, Ignacio

Subjects
*GENE targeting, *LABORATORY rats, *GENETIC transcription, *GENETIC regulation, *NUCLEASE genetics, *DNA-binding proteins
Abstract

The rat is a model of choice to understanding gene function and modeling human diseases. Since recent years, successful engineering technologies using gene-specific nucleases have been developed to gene edit the genome of different species, including the rat. This development has become important for the creation of new rat animals models of human diseases, analyze the role of genes and express recombinant proteins. Transcription activator-like (TALE) nucleases are designed nucleases consist of a DNA binding domain fused to a nuclease domain capable of cleaving the targeted DNA. We describe a detailed protocol for generating knockout rats via microinjection of TALE nucleases into fertilized eggs. This technology is an efficient, cost- and time-effective method for creating new rat models. [ABSTRACT FROM AUTHOR]

Published
2014
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3. Genome Editing in Rats Using TALE Nucleases

Author

Chloé Savignard, Laurent Tesson, Jean-Paul Concordet, Carine Giovannangeli, Séverine Ménoret, Claire Usal, Anne De Cian, Ignacio Anegon, Séverine Remy, Reynald Thinard, Le Bihan, Sylvie, Centre de Recherche en Transplantation et Immunologie (U1064 Inserm - CRTI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), Institut de transplantation urologie-néphrologie (ITUN), Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes), Structure et Instabilité des Génomes (STRING), Muséum national d'Histoire naturelle (MNHN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), and Region Pays de la Loire, Biogenouest, IBiSA, and TEFOR (Investissements d’Avenir, French government).

Subjects
0301 basic medicine, Homology-directed repair, Knock-in, Knockout rat, Knockout, Hprt, Computational biology, Gene editing, Biology, Animals, Genetically Modified, Targeted integration, Homology directed repair, Gene Knockout Techniques, 03 medical and health sciences, TALEN, Genome editing, Gene knockin, Animals, Humans, RNA, Messenger, Homologous Recombination, Gene, Transcription activator-like effector nuclease, Genome, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, Gene targeting, Genomics, TALE nucleases, Endonucleases, Rosa26, Rats, 3. Good health, 030104 developmental biology, Nonhomologous end joining, Genetic engineering, Trans-Activators, Rat model, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract

International audience; The rat is an important animal model to understand gene function and model human diseases. Since recent years, the development of gene-specific nucleases has become important for generating new rat models of human diseases, to analyze the role of genes and to generate human antibodies. Transcription activator-like (TALE) nucleases efficiently create gene-specific knockout rats and lead to the possibility of gene targeting by homology-directed recombination (HDR) and generating knock-in rats. We describe a detailed protocol for generating knockout and knock-in rats via microinjection of TALE nucleases into fertilized eggs. This technology is an efficient, cost- and time-effective method for creating new rat models.

Published
2016
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4. Gene targeting in rats using transcription activator-like effector nucleases

Author

Ignacio Anegon, Séverine Ménoret, Jean-Paul Concordet, Laurent Tesson, Séverine Remy, Laure-Hélène Ouisse, Anne De Cian, Reynald Thinard, Claire Usal, Virginie Thepenier, Carine Giovannangeli, Centre de Recherche en Transplantation et Immunologie (U1064 Inserm - CRTI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), Institut de transplantation urologie-néphrologie (ITUN), Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes), Régulation et dynamique des génomes, Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Region Pays de la Loire., Biogenouest., IBiSA., TEFOR (Investissements d’Avenir, French government)., and Le Bihan, Sylvie

Subjects
Knockout rat, DNA End-Joining Repair, Microinjections, Knockout, Biology, Gene editing, General Biochemistry, Genetics and Molecular Biology, Genome engineering, Rats, Sprague-Dawley, chemistry.chemical_compound, Gene Knockout Techniques, Genome editing, TAL effector, Animals, Protein–DNA interaction, Homologous Recombination, Molecular Biology, Genetics, Transcription activator-like effector nuclease, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, Deoxyribonucleases, Gene targeting, TALE nucleases, Embryo Transfer, Embryo, Mammalian, Cell biology, Rats, chemistry, Rat model, Mutagenesis, Site-Directed, Female, Non-homologous end joining, DNA, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract

International audience; The rat is a model of choice to understanding gene function and modeling human diseases. Since recent years, successful engineering technologies using gene-specific nucleases have been developed to gene edit the genome of different species, including the rat. This development has become important for the creation of new rat animals models of human diseases, analyze the role of genes and express recom-binant proteins. Transcription activator-like (TALE) nucleases are designed nucleases consist of a DNA binding domain fused to a nuclease domain capable of cleaving the targeted DNA. We describe a detailed protocol for generating knockout rats via microinjection of TALE nucleases into fertilized eggs. This technology is an efficient, cost-and time-effective method for creating new rat models.

Published
2013
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