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Your search keyword '"Zhou, Ruoji"' showing total 3 results
Start Over Author "Zhou, Ruoji" Topic gene editing
3 results on '"Zhou, Ruoji"'

1. Generation of a heterozygous p53 R249S mutant human embryonic stem cell line by TALEN-mediated genome editing.

Author

Huo Z, Tu J, Xu A, Li Y, Wang D, Liu M, Zhou R, Zhu D, Lin Y, Gingold JA, Yen CJ, Xiao H, and Zhao R

Subjects
Animals, Base Sequence, Cell Line, Female, Heterozygote, Humans, Mice, Cell Culture Techniques methods, Gene Editing, Human Embryonic Stem Cells metabolism, Mutation genetics, Transcription Activator-Like Effector Nucleases metabolism, Tumor Suppressor Protein p53 genetics
Abstract

As one of the most essential genome guardians, p53 and its mutants have been suggested associated with many types of cancers. Many p53 mutants function induce unique phenotypes, including carcinogenesis, metastasis, and drug resistance. The p53(R249S) mutation is the most prevalent and specific mutation associated with liver cancer development. Here, we demonstrate the generation of a heterozygous p53(R249S) mutation in the H9 human embryonic stem cell line using TALEN-mediated genome editing. The generated cell line maintains a normal karyotype, a pluripotent state and the in vivo capacity to develop a teratoma containing all three germ layer tissues., (Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.)

Published
2019
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2. Establishment of a human embryonic stem cell line with homozygous TP53 R248W mutant by TALEN mediated gene editing.

Author

Xu A, Zhou R, Tu J, Huo Z, Zhu D, Wang D, Gingold JA, Mata H, Rao PH, Liu M, Mohamed AMT, Kong CSL, Jewell BE, Xia W, Zhao R, Hung MC, and Lee DF

Subjects
Cell Line, Gene Expression Regulation, Human Embryonic Stem Cells cytology, Humans, Male, Tumor Suppressor Protein p53 metabolism, Gene Editing, Homozygote, Human Embryonic Stem Cells metabolism, Mutation, Transcription Activator-Like Effector Nucleases, Tumor Suppressor Protein p53 genetics
Abstract

Genetic mutations in TP53 contribute to multiple human cancers. Here we report the generation of a H1-p53(R248W/R248W) human embryonic stem cell line harboring a homozygous TP53 R248W mutation created by TALEN-mediated precise gene editing. The H1-p53(R248W/R248W) cell line maintains a normal karyotype, robust pluripotency gene expression, and the potential to differentiate to the three germ layers., (Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.)

Published
2018
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3. A homozygous p53 R282W mutant human embryonic stem cell line generated using TALEN-mediated precise gene editing.

Author

Zhou R, Xu A, Wang D, Zhu D, Mata H, Huo Z, Tu J, Liu M, Mohamed AMT, Jewell BE, Gingold J, Xia W, Rao PH, Hung MC, Zhao R, and Lee DF

Subjects
Homozygote, Humans, Male, Mutation genetics, Transcription Activator-Like Effector Nucleases genetics, Gene Editing methods, Human Embryonic Stem Cells metabolism, Transcription Activator-Like Effector Nucleases metabolism, Tumor Suppressor Protein p53 genetics
Abstract

The tumor suppressor gene TP53 is the most frequently mutated gene in human cancers. Many hot-spot mutations of TP53 confer novel functions not found in wild-type p53 and contribute to tumor development and progression. We report on the generation of a H1 human embryonic stem cell line carrying a homozygous TP53 R282W mutation using TALEN-mediated genome editing. The generated cell line demonstrates normal karyotype, maintains a pluripotent state, and is capable of generating a teratoma in vivo containing tissues from all three germ layers., (Copyright © 2018 The Author(s). Published by Elsevier B.V. All rights reserved.)

Published
2018
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