1. Helicobacter pylori promotes angiogenesis depending on Wnt/beta-catenin-mediated vascular endothelial growth factor via the cyclooxygenase-2 pathway in gastric cancer
- Author
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Ni Chai, Xuan Liu, Ningning Liu, Haili Jiang, Qi Li, Ning Zhou, and Qiong Wu
- Subjects
0301 basic medicine ,Vascular Endothelial Growth Factor A ,Cancer Research ,Beta-catenin ,Angiogenesis ,medicine.medical_treatment ,Gene Expression ,Helicobacter Infections ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Stomach Neoplasms ,Cell Line, Tumor ,medicine ,Genetics ,Humans ,Wnt Signaling Pathway ,beta Catenin ,biology ,Helicobacter pylori ,Neovascularization, Pathologic ,business.industry ,Growth factor ,digestive, oral, and skin physiology ,Wnt signaling pathway ,Wnt/beta-catenin ,Cancer ,biology.organism_classification ,medicine.disease ,digestive system diseases ,Up-Regulation ,Vascular endothelial growth factor ,Gene Expression Regulation, Neoplastic ,Vascular endothelial growth factor A ,030104 developmental biology ,chemistry ,Oncology ,Cyclooxygenase 2 ,030220 oncology & carcinogenesis ,Immunology ,biology.protein ,Cancer research ,business ,Gastric cancer ,Research Article - Abstract
Background Helicobacter pylori is an important pathogenic factor in gastric carcinogenesis. Angiogenesis (i.e., the growth of new blood vessels) is closely associated with the incidence and development of gastric cancer. Our previous study found that COX-2 stimulates gastric cancer cells to induce expression of the angiogenic growth factor VEGF through an unknown mechanism. Therefore, the aim of this study was to clarify the role of angiogenesis in H. pylori-induced gastric cancer development. Methods To clarify the relationship between H. pylori infection and angiogenesis, we first investigated H. pylori colonization, COX-2, VEGF, beta-catenin expression, and microvessel density (MVD) in gastric cancer tissues from 106 patients. In addition, COX-2, phospho-beta-catenin, and beta-catenin expression were measured by western blotting, and VEGF expression was measured by ELISA in H. pylori-infected SGC7901 and MKN45 human gastric cancer cells. Results H. pylori colonization occurred in 36.8 % of gastric carcinoma samples. Furthermore, COX-2, beta-catenin, and VEGF expression, and MVD were significantly higher in H. pylori-positive gastric cancer tissues than in H. pylori-negative gastric cancer tissues (P
- Published
- 2016