1. The expression of intronic miRNAs, miR-483 and miR-483*, and their host gene, Igf2, in murine osteoarthritis cartilage.
- Author
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Qi, Yuebin, Ma, Ning, Yan, Feng, Yu, Zhange, Wu, Guodong, Qiao, Yu, Han, Dong, Xiang, Ying, Li, Fuyuan, Wang, Wenbo, and Gao, Xu
- Subjects
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MICRORNA , *GENE expression , *INTRONS , *OSTEOARTHRITIS diagnosis , *CYTOKINES , *LABORATORY mice - Abstract
Abstract: MicroRNAs (miRNAs) have been shown to be important regulators in the pathogenesis of osteoarthritis (OA). The objective of this study was to determine the expression levels of miR-483, miR-483*, their host gene (Igf2) and other cytokines in a murine model of OA. The expression of miR-483 was significantly up-regulated in old mouse and in all of the operation groups, particularly the group assessed 1 week after surgery. The expression of miR-483* was significantly increased in the old mouse group and the group assessed 5 weeks after surgery. The expression of miR-483 was negatively correlated with the expression of (mRNA) Bmp7 and TgfB and positively correlated with Mmp13 by Pearson correlation analysis, while miR-483* was positively correlated with Il1B. Surprisingly, there was no correlation between the expression of either miR-483 or miR-483* and Igf2. This study shows that the expression of miR-483 and miR-483* is up-regulated in murine OA. These data suggest that miR-483 and miR-483* may play critical roles in early and later pathogenesis of OA, respectively, without the involvement of their host gene Igf2. [Copyright &y& Elsevier]
- Published
- 2013
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