1. A Decrease of Histone Deacetylase 6 Expression Caused by Helicobacter Pylori Infection is Associated with Oncogenic Transformation in Gastric Cancer
- Author
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De-Dong Yu, Guoqiang Li, Lang Yang, Yuanming Pan, Shu-Bin Wang, He Yuqi, Qing He, Xin Wang, Jiang Hu, and Yun Wu
- Subjects
0301 basic medicine ,Male ,Pathology ,Physiology ,Atrophic gastritis ,HP infection ,Gene Expression ,Histone Deacetylase 1 ,medicine.disease_cause ,Histone Deacetylase 6 ,lcsh:Physiology ,Cohort Studies ,Mice ,0302 clinical medicine ,Precancerous lesion ,lcsh:QD415-436 ,biology ,lcsh:QP1-981 ,Intestinal metaplasia ,Middle Aged ,Prognosis ,Cell Transformation, Neoplastic ,030220 oncology & carcinogenesis ,Gastritis ,Disease Progression ,Immunohistochemistry ,Female ,medicine.medical_specialty ,Histone Deacetylases ,Helicobacter Infections ,lcsh:Biochemistry ,03 medical and health sciences ,Stomach Neoplasms ,medicine ,Biomarkers, Tumor ,Animals ,Humans ,Aged ,Helicobacter pylori ,business.industry ,Cancer ,HDAC6 ,medicine.disease ,biology.organism_classification ,HDAC4 ,Survival Analysis ,Repressor Proteins ,030104 developmental biology ,Dysplasia ,Gastric Mucosa ,Chronic Disease ,Cancer research ,business ,Carcinogenesis ,Gastric cancer - Abstract
Background: Histone deacetylase 6 (HDAC6) plays a role in the progression of many tumors. However, the relationship between the level of HDAC6 expression and gastric tumorigenesis is still unclear. Here, we illustrate the potential correlation between Helicobacter pylori (HP) infection and the variation of HDAC6 expression in different gastric lesions, as well as the clinical significance of HDAC6 expression in gastric cancer (GC) patients. Materials and Methods: Between 2011 and 2016, 364 patients with different types of gastric lesions were enrolled in Baotou City Central Hospital. Immunostaining of HDAC6 expression and HP infection were performed in the following cohort including 21 normal tissues (Normal); 40 samples with chronic superficial gastritis (CSG); 106 with chronic atrophic gastritis (CAG); 94 with intestinal metaplasia (IM); 64 with dysplasia (DYS) and 39 with gastric cancer (GC). Survival analysis was performed in another 80 GC patients using the Kaplan-Meier method and multivariate Cox regression analyses. The level of HDAC6 expression was determined by Real-time PCR, Western blotting and IHC staining in gastric cell lines and tissues. Furthermore, the correlation between HDAC6 expression and clinicopathological features and prognosis was analyzed in the GC cohort. HP strains were lavaged into Kunming mice to investigate the effects of HP infection on the expression of different HDAC members in this mouse model. Results: Higher levels of HDAC6 expression were detected in normal and premalignant lesions than in the GC tissues (p
- Published
- 2016