1. Hsa-miR-1269a up-regulation fosters the malignant progression of esophageal squamous cell carcinoma via targeting FAM46C.
- Author
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Ma, Yuefeng, Xing, Xin, Cheng, Chuantao, Kong, Ranran, Sun, Liangzhang, Zhao, Feng, Zhang, Danjie, and Li, Jianzhong
- Subjects
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SQUAMOUS cell carcinoma , *ESOPHAGEAL cancer , *GENE expression , *ALIMENTARY canal - Abstract
Esophageal squamous cell carcinoma (ESCC) is a malignancy of the alimentary tract resulting in death worldwide. The role and underlying mechanism of hsa-miR-1269a in the progression of ESCC remain unclear. In this study, hsa-miR-1269a was screened by differential expression analysis in TCGA, and its target gene FAM46C was predicted. qRT-PCR was conducted to assay the expression of hsa-miR-1269a and FAM46C in ESCC cells. The results showed that hsa-miR-1269a was upregulated in ESCC tissues and cell lines. Hsa-miR-1269a overexpression stimulated the proliferation, migration, and invasion capacities of ESCC cells, and FAM46C overexpression inhibited these phenotypes. Dual-luciferase assay verified that hsa-miR-1269a could target FAM46C. Next, qRT-PCR and western blot demonstrated that hsa-miR-1269a overexpression downregulated FAM46C. Rescue experiments revealed that hsa-miR-1269a accelerated the malignant progression of ESCC through FAM46C down-regulation. These results indicate that the interaction between hsa-miR-1269a and FAM46C plays a regulatory role in driving the malignant progression of ESCC cells, thereby providing a novel molecular mechanism for understanding ESCC. • MiR-1269a was up-regulated while FAM46C was down-regulated in esophageal cancer. • MiR-1269a facilitated cell proliferation, migration, invasion, and restrained cell apoptosis of esophageal cancer. • MiR-1269a suppressed FAM46C in esophageal cancer. • MiR-1269a exacerbated progression of esophageal cancer via targeting FAM46C. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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