8 results on '"Guo, Liyuan"'
Search Results
2. A Systematic Analysis Revealed the Potential Gene Regulatory Processes of ATRA-Triggered Neuroblastoma Differentiation and Identified a Novel RA Response Sequence in the NTRK2 Gene.
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Guo, Liyuan, Lin, Wei, Zhang, Yidan, and Wang, Jing
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CELL differentiation , *CELL lines , *GENE expression , *NEUROBLASTOMA , *NEURONS , *POLYMERASE chain reaction , *PROMOTERS (Genetics) , *TRANSFERASES , *TRETINOIN , *WESTERN immunoblotting , *BIOINFORMATICS , *GENOMICS , *REVERSE transcriptase polymerase chain reaction , *PRECIPITIN tests , *SEQUENCE analysis - Abstract
Retinoic acid- (RA-) triggered neuroblastoma cell lines are widely used cell modules of neuronal differentiation in neurodegenerative disease studies, but the gene regulatory mechanism underlying differentiation is unclear now. In this study, system biological analysis was performed on public microarray data from three neuroblastoma cell lines (SK-N-SH, SH-SY5Y-A, and SH-SY5Y-E) to explore the potential molecular processes of all-trans retinoic acid- (ATRA-) triggered differentiation. RT-qPCR, functional genomics analysis, western blotting, chromatin immunoprecipitation (ChIP), and homologous sequence analysis were further performed to validate the gene regulation processes and identify the RA response element in a specific gene. The potential disturbed biological pathways (111 functional GO terms in 14 interactive functional groups) and gene regulatory network (10 regulators and 71 regulated genes) in neuroblastoma differentiation were obtained. 15 of the 71 regulated genes are neuronal projection-related. Among them, NTRK2 is the only one that was dramatically upregulated in the RT-qPCR test that we performed on ATRA-treated SH-SY5Y-A cells. We further found that the overexpression of the NTRK2 gene can trigger differentiation-like changes in SH-SY5Y-A cells. Functional genomic analysis and western blotting assay suggested that, in neuroblastoma cells, ATRA may directly regulate the NTRK2 gene by activating the RA receptor (RAR) that binds in its promoter region. A novel RA response DNA element in the NTRK2 gene was then identified by bioinformatics analysis and chromatin immunoprecipitation (ChIP) assay. The novel element is sequence conservation and position variation among different species. Our study systematically provided the potential regulatory information of ATRA-triggered neuroblastoma differentiation, and in the NTRK2 gene, we identified a novel RA response DNA element, which may contribute to the differentiation in a human-specific manner. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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3. Common variants near IKZF1 are associated with primary Sjögren's syndrome in Han Chinese.
- Author
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Qu, Susu, Du, Yang, Chang, Suhua, Guo, Liyuan, Fang, Kechi, Li, Yongzhe, Zhang, Fengchun, Zhang, Kunlin, and Wang, Jing
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AUTOIMMUNE diseases ,DISEASE susceptibility ,IKAROS transcription factors ,SINGLE nucleotide polymorphisms ,DEACETYLASES - Abstract
Primary Sjögren's syndrome (pSS) is a systematic autoimmune disease with evidence of genetic predisposition. The IKZF1 (IKAROS family zinc finger 1 (Ikaros)) gene is located at 7p12.2, encodes a transcription factor related to chromatin remodeling, regulates lymphocyte differentiation, and has been reported to be associated with some autoimmune diseases. However, there have been no reports of an association between IKZF1 and pSS. To investigate the possibility of an association between the IKZF1 locus and pSS, we selected two single nucleotide polymorphisms (SNPs) in the IKZF1 locus, rs4917129 and rs4917014, based on a detailed analysis of genome-wide association study (GWAS) data and performed genotyping in 665 Han Chinese pSS patients and 863 healthy controls. The results of an association test showed significant association signals (rs4917129: P-value = 5.5e-4, OR (odds ratio) = 0.72, 95% CI (confidence interval) = 0.60–0.87; rs4917014: P-value = 1.2e-3, OR = 0.76, 95% CI = 0.64–0.89). A meta-analysis that combined the above results with data from previous GWAS, further confirmed these associations (rs4917129: P
meta = 4.24e-8, ORmeta = 0.70, 95% CI = 0.61–0.79; rs4917014: Pmeta = 6.0e-8, ORmeta = 0.72, 95% CI = 0.64–0.81). A bioinformatics analysis indicated that both SNPs were located in a putative enhancer area in immune-related cell lines and tissues. A protein-protein interaction analysis found that IKZF1, together with GTF2I (an SS susceptibility gene newly identified through GWAS), could interact with histone deacetylase family proteins. In summary, this is the first study to report an association between IKZF1 and SS in Han Chinese. [ABSTRACT FROM AUTHOR]- Published
- 2017
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4. Genetic and epigenetic X-chromosome variations in a parthenogenetic human embryonic stem cell line.
- Author
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Liu, Weiqiang, Yin, Yifei, Jiang, Yonghua, Kou, Chaohui, Luo, Yumei, Huang, Shengchang, Zheng, Yuhong, Li, Shaoying, Li, Qing, Guo, Liyuan, Gao, Shaorong, and Sun, Xiaofang
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X chromosome ,BIOLOGICAL variation ,EMBRYONIC stem cells ,GENE expression ,KARYOTYPES ,GENOMICS ,FERTILIZATION in vitro - Abstract
Purpose: To assess the genetic and epigenetic status of parthenogenetic human embryonic stem cells (phESCs). Methods: Cytogenetics, X chromosome inactivation (XCI) and gene expression patterns were analyzed in one phESC line (FY-phES-018) that was derived from our laboratory. Results: FY-phES-018 cells displayed the classical characteristics of normal hESCs. These cells had a 46, XX karyotype, and no inactive X chromosomes were observed before passage 20. After being cultured long term in vitro, some cells lost one X, and the proportion of cells with only one X gradually increased. At passage 35, almost all the cells displayed a 45, XO karyotype. Interestingly, at passage 45, the recovery of the X-chromosome was observed, and XCI became detectable; the mosaic ratio of 46, XX to 45, XO was 67:33. After passage 60, most cells displayed the 46, XX karyotype again with a mosaic ratio of 97:3. Some aberrant genomic imprinting was also observed in these cells. Conclusions: The phESCs line FY-phES-018 is both genetically and epigenetically unstable; therefore, further research is needed before using these cells. [ABSTRACT FROM AUTHOR]
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- 2011
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5. Involvement of STAT5a signaling in morphine-induced up-regulation of the cyclin D1
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Guo, Liyuan, Li, Hui, Liu, Han, Li, Chaoying, Li, Mengsen, Jiang, Wei, He, Peng, Wang, Shanshan, McNutt, Michael A., and Li, Gang
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CELLULAR signal transduction , *CYCLINS , *OPIOID receptors , *CYTOKINES , *GENE expression , *IMMUNOREGULATION - Abstract
Abstract: Opioid receptors and cytokine receptor have been verified to have a functional link and interaction. However, the pathway by which opioid receptor in lymphocytes is linked to cytokine signaling is not well defined. Using confocal microscopy and Western blotting this study showed that morphine treatment was able to activate cytoplasmic STAT5a in CEM x174 cells, which then translocated into the nucleus and bound to elements of the cyclin D1 promoter. As a consequence the expression of the cyclin D1 was apparently up-regulated. The data from EMSA–superEMSA and ChIP-qPCR further confirmed that morphine was capable of promoting the binding of STAT5a to its elements (proximal and distal), and this was abolished by the antagonist naloxone. As shown by transient transfection assay, activity of the cyclin D1 promoter was significantly reduced by 82% (distal) and 65% (proximal) after two STAT5a elements were mutated in comparison with wild type STAT5a elements. Moreover, knockdown of STAT5a was associated with a concurrent silencing of morphine-induced expression of cyclin D1, demonstrating involvement of STAT5a in morphine-triggered signaling in the regulation of cyclin D1 expression. The finding provides evidence which demonstrates that there is cross-talk between the mu opioid receptor and cytokine signaling in lymphocytes. Thus, we conclude that morphine may modulate cyclin D1 gene expression via signal transducers and activators of transcription (STATs) signaling, which will be beneficial for further understanding of the pharmacological effect of morphine on immune regulation. [Copyright &y& Elsevier]
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- 2009
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6. BEST: a web server for brain expression Spatio-temporal pattern analysis.
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Guo, Liyuan, Lin, Wei, Zhang, Yidan, Li, Wenhan, and Wang, Jing
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INTERNET servers , *GENE expression , *MOLECULAR biologists , *GENE regulatory networks , *GENETIC correlations , *SYSTEM identification - Abstract
Background: Dysregulated gene expression patterns have been reported in several mental disorders. Limited by the difficulty of obtaining samples, psychiatric molecular mechanism research still relies heavily on clues from genetics studies. By using reference data from brain expression studies, multiple types of comprehensive gene expression pattern analysis have been performed on psychiatric genetic results. These systems-level spatial-temporal expression pattern analyses provided evidence on specific brain regions, developmental stages and molecular pathways that are possibly involved in psychiatric pathophysiology. At present, there is no online tool for such systematic analysis, which hinders the applications of analysis by non-informatics researchers such as experimental biologists and clinical molecular biologists. Results: We developed the BEST web server to support Brain Expression Spatio-Temporal pattern analysis. There are three highlighted features of BEST: 1) visualization: it generates user-friendly visual results that are easy to interpret, including heatmaps, Venn diagrams, gene co-expression networks and cluster-based Manhattan gene plots; these results illustrate the complex spatio-temporal expression patterns, including expression quantification and correlation between genes; 2) integration: it provides comprehensive human brain spatio-temporal expression patterns by integrating data from currently available databases; 3) multi-dimensionality: it analyses input genes as both a whole set and several subsets (clusters) which are enriched according to co-expression patterns, and it also presents the correlation between genetic and expression data. Conclusions: To the best of our knowledge, BEST is the first data tool to support comprehensive human brain spatial-temporal expression pattern analysis. It helps to bridge disease-related genetic studies and mechanism studies, provides clues for key gene and molecular system identification, and supports the analysis of disease sensitive brain region and age stages. BEST is freely available at http://best.psych.ac.cn. [ABSTRACT FROM AUTHOR]
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- 2019
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7. Mechanisms involved in phosphatidylinositol 3-kinase pathway mediated up-regulation of the mu opioid receptor in lymphocytes
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Liu, Han, Li, Hui, Guo, Liyuan, Li, Mengsen, Li, Chaoying, Wang, Shanshan, Jiang, Wei, Liu, Xinhua, McNutt, Michael A., and Li, Gang
- Abstract
Abstract: Despite the substantial progress made in understanding initiation expression of the MOR gene in lymphocytes, the signal pathway associated with MOR gene transcription remains to be better defined. As the phosphatidylinositol 3-kinase (PI3K)/AKT pathway can mediate diverse biological responses and is crucial for optimal immune responses and lymphocyte development, this study was undertaken to delineate the role of PI3K/AKT signaling in expression of the MOR gene in CEM ×174 cells. The data show that morphine treatment enhanced the level of phosphorylated, rather than un-phosphorylated, PI3K and AKT, which were synchronously recruited to membrane. The levels of PTEN and p53 which are negative regulators of these signal molecules were reduced, and as a result, the interaction between PTEN and p53 was completely interrupted. With morphine treatment, the levels of both cytoplasmic and nuclear E2F1 which is the downstream effecter of AKT were elevated and the interaction of E2F1with YY1, rather than Sp1, was also increased. Subsequently, E2F1 triggered the transcription of the MOR gene through its enhanced ability to bind the element in promoter region of the MOR gene. All responses to morphine were abolished by naloxone, which is an antagonist of MOR, or by LY294002, an inhibitor of PI3K, implying specific involvement of PI3K/AKT. These results strongly suggest that the PI3K/AKT pathway plays a critical role in the transfer of signal from morphine stimuli to the machinery by which MOR gene transcription is initiated. [Copyright &y& Elsevier]
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- 2010
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8. A combined analysis of genome-wide expression profiling of bipolar disorder in human prefrontal cortex.
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Wang, Jinglu, Qu, Susu, Wang, Weixiao, Guo, Liyuan, Zhang, Kunlin, Chang, Suhua, and Wang, Jing
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GENETICS of bipolar disorder , *HUMAN genome , *PREFRONTAL cortex , *GENE expression profiling , *HUMAN genetic variation , *PROTEIN-protein interactions , *PHYSIOLOGY - Abstract
Numbers of gene expression profiling studies of bipolar disorder have been published. Besides different array chips and tissues, variety of the data processes in different cohorts aggravated the inconsistency of results of these genome-wide gene expression profiling studies. By searching the gene expression databases, we obtained six data sets for prefrontal cortex (PFC) of bipolar disorder with raw data and combinable platforms. We used standardized pre-processing and quality control procedures to analyze each data set separately and then combined them into a large gene expression matrix with 101 bipolar disorder subjects and 106 controls. A standard linear mixed-effects model was used to calculate the differentially expressed genes (DEGs). Multiple levels of sensitivity analyses and cross validation with genetic data were conducted. Functional and network analyses were carried out on basis of the DEGs. In the result, we identified 198 unique differentially expressed genes in the PFC of bipolar disorder and control. Among them, 115 DEGs were robust to at least three leave-one-out tests or different pre-processing methods; 51 DEGs were validated with genetic association signals. Pathway enrichment analysis showed these DEGs were related with regulation of neurological system, cell death and apoptosis, and several basic binding processes. Protein-protein interaction network further identified one key hub gene. We have contributed the most comprehensive integrated analysis of bipolar disorder expression profiling studies in PFC to date. The DEGs, especially those with multiple validations, may denote a common signature of bipolar disorder and contribute to the pathogenesis of disease. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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