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Your search keyword '"Hideo Inagaki"' showing total 2 results
Start Over Author "Hideo Inagaki" Topic gene expression
2 results on '"Hideo Inagaki"'

1. Difference in the ligand affinity among redundant plant hormone receptors of rice OsCOI1a/1b/2-OsJAZs.

Author

Taichi Okumura, Tsumugi Kitajima, Takuya Kaji, Haruyuki Urano, Kotaro Matsumoto, Hideo Inagaki, Koji Miyamoto, Kazunori Okada, and Minoru Ueda

Subjects
HORMONE receptors, RICE, PROTEIN-protein interactions, ABIOTIC stress, GENE expression, JASMONATE, PLANT hormones
Abstract

(3R, 7S)-jasmonoyl-L-isoleucine (JA-Ile) is a lipid-derived plant hormone that regulates plant responses, including biotic/abiotic stress adaptation. In the plant cells, JA-Ile is perceived by COI1-JAZ co-receptor by causing protein-protein interaction between COI1 and JAZ proteins to trigger gene expressions. In this study, we focused on Oryza sativa, a model monocot and an important crop, with 45 possible OsCOI-OsJAZ co-receptor pairs composed of three OsCOI homologs (OsCOI1a, OsCOI1b, and OsCOI2) and 15 OsJAZ homologs. We performed fluorescein anisotropy and pull-down assays to examine the affinity between JA-Ile and OsCOI1a/1b/2-OsJAZ1-15 co-receptor pairs. The results revealed a remarkable difference in the modes of ligand perception by OsCOI1a/1b and OsCOI2. Recently, the unique function of OsCOI2 in some of the JA-responses were revealed. Our current results will lead to the possible development of OsCOI2-selective synthetic ligand. [ABSTRACT FROM AUTHOR]

Published
2023
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2. [V-1, a catecholamine biosynthesis regulatory protein, enhances GTP cyclohydrolase I gene transcription in a cAMP-responsive element dependent manner]

Author

Tohru, Yamakuni, Takahiro, Suzuki, Hideo, Inagaki, Yasushi, Ohizumi, Toshiharu, Nagatsu, and Hiroshi, Ichinose

Subjects
Mice, Transcription, Genetic, Animals, Gene Expression, Intercellular Signaling Peptides and Proteins, Mice, Transgenic, Nerve Tissue Proteins, Cyclic AMP Response Element-Binding Protein, GTP Cyclohydrolase, Biopterin, Cell Line
Abstract

V-1 is a 12 kDa protein containing 2.5 copies of the ankyrin repeat, which has been demonstrated to be required for protein-protein interactions. Recently we have for the first time reported that stable overexpression of V-1 enhances mRNA expression of catecholamine synthesizing enzymes in PC12D cells, and as a result, catecholamine production is upregulated. GTP cyclohydrolase I (GCH) is the enzyme in the first and rate-limiting step for the biosynthesis of tetrahydrobiopterin (BH4) which is an essential cofactor for tyrosine hydroxylase. In the present study, to examine further the function of V-1 in control of the BH4 biosynthesis, we assayed BH4 content and GCH enzyme activity in V-1-overexpressing PC12D cell clones. It was shown that both BH4 content and GCH enzyme activity were increased in V-1-verexpressing PC12D cell clones. It was also revealed that V-1-overexpression caused augmentation of both the GCH protein and mRNA expression and the cAMP-responsive element (CRE) dependent transcription. Furthermore, promoter analysis showed an increased activity in the construct with 150 bp of promoter region of the human GCH gene in the V-1-overexpressing clones. These results suggest that V-1 promotes GCH gene expression via a CRE-dependent transcription to positively control the BH4 biosynthesis in catecholaminergic cells.

Published
2002

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