1. Expression of Rab27B-binding protein Slp1 in pancreatic acinar cells and its involvement in amylase secretion.
- Author
-
Saegusa C, Kanno E, Itohara S, and Fukuda M
- Subjects
- Animals, Membrane Proteins genetics, Mice, Mice, Knockout, Pancreas, Exocrine cytology, Secretory Vesicles metabolism, Vesicular Transport Proteins, rab GTP-Binding Proteins genetics, Amylases metabolism, Gene Expression, Membrane Proteins metabolism, Pancreas, Exocrine physiology, rab GTP-Binding Proteins metabolism
- Abstract
Slp1 is a putative Rab27 effector protein and implicated in intracellular membrane transport; however, the precise tissue distribution and function of Slp1 protein remain largely unknown. In this study we investigated the tissue distribution of Slp1 in mice and found that Slp1 is abundantly expressed in the pancreas, especially in the apical region of pancreatic acinar cells. Slp1 interacted with Rab27B in vivo and both proteins were co-localized on zymogen granules. Morphological analysis of fasted Slp1 knockout mice showed an increased number of zymogen granules in the pancreatic acinar cells, indicating that Slp1 is part of the machinery of amylase secretion by the exocrine pancreas.
- Published
- 2008
- Full Text
- View/download PDF