1. Establishment and characteristics of white ear lobe chicken embryo fibroblast line and expression of six fluorescent proteins in the cells.
- Author
-
Wu H, Guan W, Li H, and Ma Y
- Subjects
- Animals, Cell Compartmentation physiology, Cell Culture Techniques, Cell Nucleus metabolism, Cell Nucleus ultrastructure, Cell Shape physiology, Cell Survival physiology, Cells, Cultured, Chick Embryo, Cytoplasm metabolism, Cytoplasm ultrastructure, Diploidy, Energy Metabolism physiology, Equipment Contamination, Fibroblasts cytology, L-Lactate Dehydrogenase metabolism, Malate Dehydrogenase metabolism, Time Factors, Cell Proliferation, Chromosomes genetics, Fibroblasts metabolism, Gene Expression genetics, Luminescent Proteins genetics
- Abstract
A white ear lobe chicken embryo (WELCE) fibroblast cell bank, containing 322 tubes of frozen cells, was successfully established from primary explants of 57 embryo samples. The cells were morphologically consistent with fibroblasts, and the growth curve was sigmoidal with a population doubling time (PDT) of 48 h. Karyotyping and G-banding indicated a total chromosome number of 2n=78; the rate of diploidy in the cell bank was 97.62%. The cells were also free from bacterial, fungal, viral and mycoplasma contamination. Analysis of lactate dehydrogenase (LDH) and malate dehydrogenase (MDH) isoenzymes ruled out cross-contamination between cells. In order to study exogenous gene expression, six fluorescent proteins were transfected into the WELCE cells. The transfection efficiency of these genes was between 10.1 and 41.9%. The corresponding fluorescence was distributed throughout the cytoplasm and nucleus 24h after transfection. The results indicate that the quality of the cell line meet the quality requirements of the ATCC (American Type Culture Collection).
- Published
- 2008
- Full Text
- View/download PDF