1. Gene expression and localization of high-mobility group box chromosomal protein-1 (HMGB-1)in human osteoarthritic cartilage.
- Author
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Terada C, Yoshida A, Nasu Y, Mori S, Tomono Y, Tanaka M, Takahashi HK, Nishibori M, Ozaki T, and Nishida K
- Subjects
- Aged, Aged, 80 and over, Animals, Cartilage, Articular cytology, Cartilage, Articular pathology, Chondrocytes cytology, Chondrocytes drug effects, Chondrocytes physiology, Humans, Interleukin-1beta metabolism, Interleukin-1beta pharmacology, Middle Aged, Osteoarthritis pathology, Receptor for Advanced Glycation End Products, Receptors, Immunologic genetics, Receptors, Immunologic metabolism, Tumor Necrosis Factor-alpha metabolism, Tumor Necrosis Factor-alpha pharmacology, Cartilage, Articular physiology, Gene Expression, HMGB1 Protein genetics, HMGB1 Protein metabolism, Osteoarthritis genetics, Osteoarthritis metabolism
- Abstract
We investigated the expression and localization of high-mobility group box chromosomal protein-1 (HMGB-1) in human osteoarthritic (OA) cartilage in relation to the histopathological grade of cartilage destruction, and examined the role of HMGB-1 in the regulation of proinflammatory cytokine expression in chondrocytes. An immunohistochemical study demonstrated that total HMGB-1-positive cell ratios increase as the Osteoarthritis Research Society International (OARSI) histological grade increased. The population of cytoplasmic HMGB-1-positive chondrocytes was especially increased in the deep layers of higher-grade cartilage. The ratios and localization of receptors for advanced glycation end products (RAGE) expression by chondrocytes in Grade 2, 3, and 4 were significantly higher than those in Grade 1. In vitro stimulation with IL-1β, but not TNFα, significantly upregulated the expression of HMGB-1 mRNA by human OA chondrocytes. Both IL-1β and TNFα promoted the translocation of HMGB-1 from nuclei to cytoplasm. IL-1β and TNFα secretions were stimulated at higher levels of HMGB-1. The results of our study suggest the involvement of HMGB-1 in the pathogenesis of cartilage destruction in OA.
- Published
- 2011
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