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3 results on '"Refik Bozbuga"'

1. Molecular investigation of proteinase inhibitor (PI) gene in tomato plants induced by Meloidogyne species

Author

Refik Bozbuga

Subjects
Gene expression, Proteinase inhibitors I gene, nematode, Tomato, Meloidogyne incognita, Meloidogyne javanica, Biotechnology, TP248.13-248.65, Genetics, QH426-470
Abstract

Abstract Background The plant parasitic nematode genus Meloidogyne parasitize almost all flowering crops. Plants respond with a variety of morphological and molecular mechanisms to reduce the effects of pathogens. Proteinase inhibitors (PI), a special group of plant proteins which are small proteins, involve in protective role in the plants attacked by microorganisms. Still, the plant response using PI against nematodes has not been well understood. Therefore, this study was aimed to determine the expression of proteinase inhibitor I (PI-I) gene subsequent the infection of M. incognita, M. javanica, and M. chitwoodi in tomato plants post nematode infections. Molecular methods were used to determine the PI gene expressions at different days post nematode infections in host tissues. Results Results revealed that the population of M. incognita species reached the highest level of nematode population followed by M. javanica and M. chitwoodi, respectively. All Meloidogyne species induced expression of PI-I gene reached at the utmost level at 3 days post infection (dpi) in host tissues. Relative gene expression level was sharply dropped at 7 dpi, 14 dpi, and 21 dpi in M. incognita induced gene expression in host tissues. Similar results were observed in host tissues after infection of M. javanica and M. chitwoodi. Conclusions The commonalities of plant response across a diverse Meloidogyne species interaction and the expression of PI gene may be related to plant defense system. Increased level of PI gene expressions in early infection days in host tissues induced by parasitic nematodes may share resemblances to the mechanisms of resistance on biotrophic interactions.

Published
2021
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2. Effect of Submerging Solanum lycopersicum Roots in Salicylic Acid (SA) Solution for Different Durations on Nematode Infection and Expressions of SlPR5 Gene

Author

Refik Bozbuga

Subjects
Agricultural, Engineering, Mühendislik, Ziraat, fungi, food and beverages, General Medicine, Biology, medicine.disease, biology.organism_classification, Gene expression,Meloidogyne incognita,PR5,Salicylic acid,Solanum lycopersicum, Microbiology, chemistry.chemical_compound, Nematode infection, chemistry, Gene expression, medicine, Meloidogyne incognita, Solanum, Gene, Salicylic acid
Abstract

Salicylic acid (SA) stimulates the mechanism of the plant defence and involves in a role in plant pathogen interactions. Plant parasitic nematodes are important biotic stresses causing negative effect on plant growth and development. Treatment of plant roots with SA may increase the plant defence mechanisms against biotic stresses. However, the treated effect of SA on plant defence mechanisms against a root-knot nematode, Meloidogyne incognita, has not been fully understood in terms of plant pathogen interactions. Therefore, this study was aimed to determine the most effective SA exposure time on increasing the plant defence and decreasing the nematode parasitism in Solanum lycopersicum. In addition, effects of SA treatment on the expression Pathogenesis Related Gene 5 (PR5) was evaluated. For this aim, tomato seedlings were exposed within 1000µM SA concentration with distinctive time durations. The expression of PR5 gene was accomplished using RT-PCR at 1, 3, 7, 14, 21 days post infection (dpi) for each sample. Root galling index, nematode number and reproduction rate were evaluated. Results revealed that nematode reproduction rate was decreased at in longer durations after SA treatment on roots. The highest nematode reproduction rate was determined in nematode+water (non-SA treatment) application compare to SA treatments. The highest increased level of expression of SlPR5 gene was determined in early (1 dpi) SA treatment + nematode infection. To conclude, SA treatment may increase the plant defence mechanisms and PR5 gene may involve in nematode-plant parasitism.

Published
2020
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3. Expressions of Pathogenesis related 1 (PR1) Gene in Solanum lycopersicum and Influence of Salicylic Acid Exposures on Host-Meloidogyne incognita Interactions

Author

Refik Bozbuga

Subjects
Biophysics, Defence mechanisms, Biology, Biochemistry, Microbiology, 03 medical and health sciences, chemistry.chemical_compound, Anti-Infective Agents, Solanum lycopersicum, Gene Expression Regulation, Plant, Gene expression, Meloidogyne incognita, medicine, Animals, Tylenchoidea, Pathogen, Gene, Plant Diseases, 030304 developmental biology, 0303 health sciences, fungi, 030302 biochemistry & molecular biology, food and beverages, General Chemistry, General Medicine, biology.organism_classification, medicine.disease, Nematode infection, chemistry, Host-Pathogen Interactions, Solanum, Salicylic Acid, Salicylic acid, Signal Transduction
Abstract

Salicylic acid (SA) induces the mechanism of the plant defence and plays a role in plant -pathogen interactions. Following the nematode infection, effects of SA treatment on plant weights and the expression of pathogen related gene have not been fully understood. The present study was aimed to determine the effects of SA treatment on the expression Pathogenesis related 1 gene (PR1 gene) and alteration on plant parameters in tomatoes (Solanum lycopersicum). Plant seedlings were dripped within the SA solution. The expression of PR1 gene achieved using RT-PCR at 1, 3, 7, 14, 21-days post infection (dpi) with Meloidogyne incognita. Upregulation of PR1 gene was determined in early (1 dpi) and late (21 dpi). SA treatment and nematode infection altered plant parameters. SA treatment increased the plant defence mechanisms in tomato against Meloidogyne incognita.

Published
2020
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