1. Simian sarcoma virus transformation of normal rat kidney fibroblasts is associated with markedly increased basic fibroblast growth factor expression.
- Author
-
Milner PG
- Subjects
- Amino Acid Sequence, Animals, Blotting, Northern, Cell Transformation, Viral, Cerebral Cortex drug effects, Chromatography, Affinity, DNA Probes, Electrophoresis, Polyacrylamide Gel, Endothelium, Vascular drug effects, Fibroblast Growth Factor 2 isolation & purification, Fibroblast Growth Factor 2 pharmacology, Fibroblasts microbiology, Kidney microbiology, Molecular Sequence Data, Molecular Weight, Neurons drug effects, RNA, Messenger analysis, Rats, Sarcoma Virus, Woolly Monkey metabolism, Fibroblast Growth Factor 2 genetics, Gene Expression, Sarcoma Virus, Woolly Monkey genetics
- Abstract
Transformation of normal rat kidney fibroblasts (NRK) by the simian sarcoma virus (SSV) occurs as a result of expression of p28v-sis, a homologue of platelet-derived growth factor-B chain. Chromatographic separation revealed that the bulk (85%) of the mitogenic activity in SSV-transformed NRK cells was not due to p28v-sis but rather two distinct endothelial cell growth factors that eluted off heparin-Sepharose between 1 and 2 M NaCl. Protein purification and Northern blot analysis revealed that one of these growth factors was the 18 kd form of bFGF, the expression of which was found to increase 15-fold with SSV-transformation of NRK cells. The pure 18 Kd bFGF had no effect on NRK cell growth but was a potent neurotrophic agent for fetal rat cortical neurones and a potent growth factor for fetal bovine heart endothelial cells, suggesting a paracrine but not autocrine role for this protein. The second endothelial cell growth factor activity in SSV-transformed NRK cells was due to an 18 Kd protein which could be distinguished immunologically, biochemically, and mitogenically from bFGF.
- Published
- 1991
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