8 results on '"Seo, Sang Beom"'
Search Results
2. Deacetylase activity-independent transcriptional activation by HDAC2 during TPA-induced HL-60 cell differentiation.
- Author
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Jung, Hyeonsoo, Kim, Ji-Young, Kim, Kee-Beom, Chae, Yun-Cheol, Hahn, Yoonsoo, Kim, Jung-Woong, and Seo, Sang-Beom
- Subjects
MYELOID leukemia ,DEACETYLASES ,CANCER cell differentiation ,MONOCYTES ,RNA sequencing ,CHROMATIN - Abstract
The human myeloid leukemia cell line HL-60 differentiate into monocytes following treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA). However, the mechanism underlying the differentiation of these cells in response to TPA has not been fully elucidated. In this study, we performed ChIP-seq profiling of RNA Pol II, HDAC2, Acetyl H3 (AcH3), and H3K27me3 and analyzed differential chromatin state changes during TPA-induced differentiation of HL-60 cells. We focused on atypically active genes, which showed enhanced H3 acetylation despite increased HDAC2 recruitment. We found that HDAC2 positively regulates the expression of these genes in a histone deacetylase activity-independent manner. HDAC2 interacted with and recruited paired box 5 (PAX5) to the promoters of the target genes and regulated HL-60 cell differentiation by PAX5-mediated gene activation. Taken together, these data elucidated the specific-chromatin status during HL-60 cell differentiation following TPA exposure and suggested that HDAC2 can activate transcription of certain genes through interactions with PAX5 in a deacetylase activity-independent pathway. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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3. Inhibition of Ku70 acetylation by INHAT subunit SET/TAF-Iβ regulates Ku70-mediated DNA damage response.
- Author
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Kim, Kee-Beom, Kim, Dong-Wook, Park, Jin, Jeon, Young-Joo, Kim, Daehwan, Rhee, Sangmyung, Chae, Jung-Il, and Seo, Sang-Beom
- Subjects
ACETYLATION ,DNA structure ,DNA damage ,CELL death ,GENOMICS ,DNA repair ,GENE expression - Abstract
DNA double-strand breaks (DSBs) can cause either cell death or genomic instability. The Ku heterodimer Ku70/80 is required for the NHEJ (non-homologous end-joining) DNA DSB repair pathway. The INHAT (inhibitor of histone acetyltransferases) complex subunit, SET/TAF-Iβ, can inhibit p300- and PCAF-mediated acetylation of both histone and p53, thereby repressing general transcription and that of p53 target genes. Here, we show that SET/TAF-Iβ interacts with Ku70/80, and that this interaction inhibits CBP- and PCAF-mediated Ku70 acetylation in an INHAT domain-dependent manner. Notably, DNA damage by UV disrupted the interaction between SET/TAF-Iβ and Ku70. Furthermore, we demonstrate that overexpressed SET/TAF-Iβ inhibits recruitment of Ku70/80 to DNA damage sites. We propose that dysregulation of SET/TAF-Iβ expression prevents repair of damaged DNA and also contributes to cellular proliferation. All together, our findings indicate that SET/TAF-Iβ interacts with Ku70/80 in the nucleus and inhibits Ku70 acetylation. Upon DNA damage, SET/TAF-Iβ dissociates from the Ku complex and releases Ku70/Ku80, which are then recruited to DNA DSB sites via the NHEJ DNA repair pathway. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
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4. H3K9 histone methyltransferase G9a-mediated transcriptional activation of p21.
- Author
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Oh, Si-Taek, Kim, Kee-Beom, Chae, Yun-Cheol, Kang, Joo-Young, Hahn, Yoonsoo, and Seo, Sang-Beom
- Subjects
HISTONE methyltransferases ,GENETIC regulation ,GENETIC transcription ,P21 gene ,APOPTOSIS ,ETOPOSIDE ,PROMOTERS (Genetics) ,GENE expression - Abstract
Highlights: [•] G9a upregulates p21 expression independently of its HMTase activity. [•] Positive regulation of p21 by G9a is p53 independent. [•] G9a interacts with PCAF to activate p21 expression. [•] G9a and PCAF are recruited to p21 promoter upon etoposide treatment. [•] G9a inhibits cell survival via p21-mediated apoptosis upon cellular stress. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
5. Peroxiredoxin 6 expression is inversely correlated with nuclear factor-κB activation during Clonorchis sinensis infestation.
- Author
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Pak, Jhang Ho, Son, Woo Chan, Seo, Sang-Beom, Hong, Sung-Jong, Sohn, Woon-Mok, Na, Byoung-Kuk, and Kim, Tong-Soo
- Subjects
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LIVER flukes , *CLONORCHIS sinensis , *PEROXIREDOXINS , *GENE expression , *NF-kappa B , *CARCINOGENESIS , *OXIDATIVE stress - Abstract
Clonorchis sinensis is a carcinogenic human liver fluke. Its infection promotes persistent oxidative stress and chronic inflammation environments in the bile duct and surrounding liver tissues owing to direct contact with worms and their excretory–secretory products (ESPs), provoking epithelial hyperplasia, periductal fibrosis, and cholangiocarcinogenesis. We examined the reciprocal regulation of two ESP-induced redox-active proteins, NF-κB and peroxiredoxin 6 (Prdx6), during C. sinensis infection. Prdx6 overexpression suppressed intracellular free-radical generation by inhibiting NADPH oxidase2 and inducible nitric oxide synthase activation in the ESP-treated cholangiocarcinoma cells, substantially attenuating NF-κB-mediated inflammation. NF-κB overexpression decreased Prdx6 transcription levels by binding to two κB sites within the promoter. This transcriptional repression was compensated for by other ESP-induced redox-active transcription factors, including erythroid 2-related factor 2 (Nrf2), hypoxia inducible factor 1α (HIF1α), and CCAAT/enhancer-binding protein β (C/EBPβ). Distribution of immunoreactive Prdx6 and NF-κB was distinct in the early stages of infection in mouse livers but shared concomitant localization in the later stages. The intensity and extent of their immunoreactive staining in infected mouse livers are proportional to lesion severity and infection duration. The constitutive elevations of Prdx6 and NF-κB during C. sinensis infection may be associated with more severe persistent hepatobiliary abnormalities mediated by clonorchiasis. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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6. Histone demethylase KDM3B regulates the transcriptional network of cell-cycle genes in hepatocarcinoma HepG2 cells.
- Author
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An, Mi-Jin, Kim, Dae-Hyun, Kim, Chul-Hong, Kim, Mijin, Rhee, Sangmyung, Seo, Sang-Beom, and Kim, Jung-Woong
- Subjects
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HISTONE demethylases , *LIVER cancer , *GENE regulatory networks , *CELL cycle , *GENE expression - Abstract
Abstract Hepatocellular carcinoma (HCC) is the fifth most common cancer and the third most lethal cancer worldwide. Although gene mutations associated with HCC development have been intensively studied, how epigenetic factors specifically modulate the functional properties of HCC by regulating target gene expression is unclear. Here we demonstrated the overexpression of KDM3B in liver tissue of HCC patients using public RNA-seq data. Ablation of KDM3B by CRISPR/Cas9 retarded the cell cycle and proliferation of hepatocarcinoma HepG2 cells. Approximately 30% of KDM3B knockout cells exhibited mitotic spindle multipolarity as a chromosome instability (CIN) phenotype. RNA-seq analysis of KDM3B knockout revealed significantly down-regulated expression of cell cycle related genes, especially cell proliferation factor CDC123. Furthermore, the expression level of Cyclin D1 was reduced in KDM3B knockout by proteosomal degradation without any change in the expression of CCND1 , which encodes Cyclin D1. The results implicate KDM3B as a crucial epigenetic factor in cell cycle regulation that manipulates chromatin dynamics and transcription in HCC, and identifies a potential gene therapy target for effective treatment of HCC. Highlights • KDM3B overexpressed in human hepatocarcinoma cells. • The ablation of KDM3B reduced proliferation of hepatocarcinoma HepG2 cells. • Protein expression level of Cyclin D1 was down-regulated in KDM3B KO cells through the proteosomal degradation pathway. • Transcript level of the cell proliferation factor CDC123 was decreased in KDM3B KO cells. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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7. Transcriptional induction of minichromosome maintenance protein 7 (Mcm7) in human cholangiocarcinoma cells treated with Clonorchis sinensis excretory–secretory products
- Author
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Kim, Dong-Wook, Kim, Ji-Young, Moon, Ju Hyun, Kim, Kee-Beom, Kim, Tong-Soo, Hong, Sung-Jong, Cheon, Young Pil, Pak, Jhang Ho, and Seo, Sang-Beom
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GENETIC transcription , *CHOLANGIOCARCINOMA , *CANCER cells , *HISTONES , *ACETYLTRANSFERASES , *MOLECULAR biology , *GENE expression - Abstract
Abstract: Clonorchiasis is an infection associated with bile duct malignancy and subsequent development of cholangiocarcinoma. This disease is mainly caused by Clonorchis sinensis worms and their excretory–secretory products (ESP). However, the precise molecular mechanisms of carcinogenesis remain to be determined. Previously, we established differential gene expression profiles from microarrays containing 23,920 human genes of known function in a human cholangiocarcinoma cell line, HuCCT1, treated with ESP. Among the upregulated genes, we focused on minichromosome maintenance protein 7 (Mcm7), which is implicated in various cancer types, and analyzed transcriptional regulation mediated by ESP to further elucidate its role in cholangiocarcinoma development. Global histone acetylation levels were increased in ESP-treated cells, along with histone acetyltransferase (HAT) protein expression. Detailed promoter analysis using reporter and chromatin immunoprecipitation assays revealed that transcriptional activation of Mcm7 is mediated by HAT recruitment to the promoter region upon C. sinensis ESP treatment. These findings contribute to clarification of the intrinsic mechanism underlying the cellular carcinogenesis process stimulated by Mcm7 in C. sinensis-treated host cells. [Copyright &y& Elsevier]
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- 2010
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8. Histone methyltransferase PRDM8 regulates mouse testis steroidogenesis
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Eom, Gwang Hyeon, Kim, Kabsun, Kim, Sung-Mi, Kee, Hae Jin, Kim, Ji-Young, Jin, Hye Mi, Kim, Ju-Ryoung, Kim, Jung Ha, Choe, Nakwon, Kim, Kee-Beom, Lee, Junwon, Kook, Hyun, Kim, Nacksung, and Seo, Sang-Beom
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HISTONES , *METHYLTRANSFERASES , *GENETIC transcription regulation , *LABORATORY mice , *TESTIS , *STEROIDS , *CHROMATIN , *BIOINFORMATICS , *ZINC-finger proteins , *GENE expression - Abstract
Abstract: A family of PRDM proteins are similar to histone methyltransferases (HMTases) with SET domain in that they modulate different cellular processes, including transcriptional regulation, through chromatin modifying activities. By applying a bioinformatic approach, we searched for proteins containing the SET domain and identified a double zinc-finger domain containing PRDM8 with HMTase activity. In vitro HMTase assay and immunoblot analysis revealed that PRDM8 specifically methylates H3K9 of histones which indicates transcriptional repression activity of PRDM8. Direct recruitment of PRDM8 to the promoter mediated transcriptional repression and indicated no involvement of HDAC. Tissue blot analyses identified PRDM8 transcripts from brain and testis in adult mouse. Consistent with these observations, we demonstrate that PRDM8 repressed the expression of steroidogenic markers, p450c17c and LHR, which indicates its regulatory role in mouse testis development. [Copyright &y& Elsevier]
- Published
- 2009
- Full Text
- View/download PDF
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