1. Gene expression changes within Müller glial cells in retinitis pigmentosa.
- Author
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Roesch K, Stadler MB, and Cepko CL
- Subjects
- Animals, Cell Death genetics, Gene Expression Profiling, Gene Knockout Techniques, Glial Fibrillary Acidic Protein, Mice, Nerve Tissue Proteins metabolism, Neuroglia pathology, Oligonucleotide Array Sequence Analysis, RNA, Messenger biosynthesis, Retina pathology, Retinal Cone Photoreceptor Cells metabolism, Retinal Cone Photoreceptor Cells pathology, Retinal Degeneration metabolism, Retinal Rod Photoreceptor Cells metabolism, Retinal Rod Photoreceptor Cells pathology, Retinitis Pigmentosa metabolism, Rhodopsin deficiency, Rhodopsin genetics, Single-Cell Analysis, Up-Regulation, Gene Expression, Nerve Tissue Proteins genetics, Neuroglia metabolism, Retina metabolism, Retinal Degeneration genetics, Retinitis Pigmentosa genetics
- Abstract
Purpose: Retinitis pigmentosa (RP) is a progressive retinal degeneration in which the retina loses nearly all of its photoreceptor cells and undergoes major structural changes. Little is known regarding the role the resident glia, the Müller glia, play in the progression of the disease. In this article, we define gene expression changes in Müller glial cells (MGCs) from two different mouse models of RP, the retinal degeneration 1 (rd1) and rhodopsin knockout (Rhod-ko) models. The RNA repertoire of single MGCs was comprehensively profiled, and a comparison was made between MGCs from wild-type (WT) and mutant retinas. Two time points were chosen for analysis, one at the peak of rod photoreceptor death and one during the period of cone photoreceptor death., Methods: Retinas were dissociated, and single MGCs were chosen under a dissecting microscope using a micropipette. Single cell cDNAs were generated and genome-wide profiles were obtained by hybridization to Affymetrix arrays. A comparison was made among all samples to discover the changes in gene expression during the periods of rod and cone photoreceptor death., Results: MGCs respond to retinal degeneration by undergoing gliosis, a process marked by the upregulation of glial fibrillary acidic protein (Gfap). Many additional transcripts were found to change. These can be placed into functional clusters, such as retinal remodeling, stress response, and immune-related response., Conclusions: A high degree of heterogeneity among the individual cells was observed, possibly due to their different spatial proximities to dying cells and/or inherent heterogeneity among MGCs.
- Published
- 2012