Search

Your search keyword '"Viral transcripts"' showing total 3 results
Start Over Descriptor "Viral transcripts" Topic gene expression
3 results on '"Viral transcripts"'

1. Exploring JC Polyomavirus Sequences and Human Gene Expression in Brain Tissue of Patients With Progressive Multifocal Leukoencephalopathy.

Author

Honkimaa, Anni, Laine, Pia, Suppula, Joni, Tynninen, Olli, Saarela, Mika, Laakso, Sini M, Hetemäki, Iivo, Liimatainen, Hanna, Auvinen, Petri, and Auvinen, Eeva

Subjects
*PROGRESSIVE multifocal leukoencephalopathy, *NEUROLOGICAL disorders, *GENE expression, *WHITE matter (Nerve tissue), *HUMAN genes, *JOHN Cunningham virus
Abstract

Progressive multifocal leukoencephalopathy (PML) is a rare neurological condition associated with reactivation of dormant JC polyomavirus (JCPyV). In this study, we characterized gene expression and JCPyV rearrangements in PML brain tissue. Infection of white matter astrocytes and oligodendrocytes as well as occasional brain cortex neurons was shown. PML brain harbored exclusively rearranged JCPyV variants. Viral transcripts covered the whole genome on both strands. Strong differential expression of human genes associated with neuroinflammation, blood-brain barrier permeability, and neurodegenerative diseases was shown. Pathway analysis revealed wide immune activation in PML brain. The study provides novel insights into the pathogenesis of PML. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

2. Differential expression of human papillomavirus 16-, 18-, 52-, and 58-derived transcripts in cervical intraepithelial neoplasia.

Author

Baba, Satoshi, Taguchi, Ayumi, Kawata, Akira, Hara, Konan, Eguchi, Satoko, Mori, Mayuyo, Adachi, Katsuyuki, Mori, Seiichiro, Iwata, Takashi, Mitsuhashi, Akira, Maeda, Daichi, Komatsu, Atsushi, Nagamatsu, Takeshi, Oda, Katsutoshi, Kukimoto, Iwao, Osuga, Yutaka, Fujii, Tomoyuki, and Kawana, Kei

Subjects
*PAPILLOMAVIRUSES, *PAPILLOMAVIRUS diseases, *CERVICAL intraepithelial neoplasia, *VIRAL proteins, *GENE expression
Abstract

Background: Human papillomavirus (HPV) infection is a primary cause of cervical cancer. Although epidemiologic study revealed that carcinogenic risk differs according to HPV genotypes, the expression patterns of HPV-derived transcripts and their dependence on HPV genotypes have not yet been fully elucidated. Methods: In this study, 382 patients with abnormal cervical cytology were enrolled to assess the associations between HPV-derived transcripts and cervical intraepithelial neoplasia (CIN) grades and/or HPV genotypes. Specifically, four HPV-derived transcripts, namely, oncogenes E6 and E6*, E1^E4, and viral capsid protein L1 in four major HPV genotypes—HPV 16, 18, 52, and 58—were investigated. Results: The detection rate of E6/E6* increased with CIN progression, whereas there was no significant change in the detection rate of E1^E4 or L1 among CIN grades. In addition, we found that L1 gene expression was HPV type-dependent. Almost all HPV 52-positive specimens, approximately 50% of HPV 58-positive specimens, around 33% of HPV 16-positive specimens, and only one HPV18-positive specimen expressed L1. Conclusions: We demonstrated that HPV-derived transcripts are HPV genotype-dependent. Especially, expression patterns of L1 gene expression might reflect HPV genotype-dependent patterns of carcinogenesis. [ABSTRACT FROM AUTHOR]

Published
2020
Full Text
View/download PDF

3. Differential expression of human papillomavirus 16-, 18-, 52-, and 58-derived transcripts in cervical intraepithelial neoplasia

Author

Atsushi Komatsu, Katsutoshi Oda, Kei Kawana, Konan Hara, Satoshi Baba, Akira Mitsuhashi, Takashi Iwata, Katsuyuki Adachi, Yutaka Osuga, Akira Kawata, Ayumi Taguchi, Seiichiro Mori, Tomoyuki Fujii, Mayuyo Mori, Daichi Maeda, Takeshi Nagamatsu, Satoko Eguchi, and Iwao Kukimoto

Subjects
0301 basic medicine, Adult, Gene Expression Regulation, Viral, Human papillomavirus, Genotype, Uterine Cervical Neoplasms, Cervix Uteri, Biology, Cervical intraepithelial neoplasia, medicine.disease_cause, lcsh:Infectious and parasitic diseases, 03 medical and health sciences, 0302 clinical medicine, Virology, Gene expression, medicine, Humans, lcsh:RC109-216, Differential expression, Papillomaviridae, Cervical cancer, Human papillomavirus 16, Research, virus diseases, Oncogene Proteins, Viral, Middle Aged, medicine.disease, Uterine Cervical Dysplasia, female genital diseases and pregnancy complications, 030104 developmental biology, Infectious Diseases, Capsid, 030220 oncology & carcinogenesis, Capsid Proteins, Female, Viral transcripts, Detection rate, Carcinogenesis
Abstract

金沢大学医薬保健研究域医学系, Background: Human papillomavirus (HPV) infection is a primary cause of cervical cancer. Although epidemiologic study revealed that carcinogenic risk differs according to HPV genotypes, the expression patterns of HPV-derived transcripts and their dependence on HPV genotypes have not yet been fully elucidated. Methods: In this study, 382 patients with abnormal cervical cytology were enrolled to assess the associations between HPV-derived transcripts and cervical intraepithelial neoplasia (CIN) grades and/or HPV genotypes. Specifically, four HPV-derived transcripts, namely, oncogenes E6 and E6*, E1^E4, and viral capsid protein L1 in four major HPV genotypes-HPV 16, 18, 52, and 58-were investigated. Results: The detection rate of E6/E6*increased with CIN progression, whereas there was no significant change in the detection rate of E1^E4 or L1 among CIN grades. In addition, we found that L1 gene expression was HPV type-dependent. Almost all HPV 52-positive specimens, approximately 50% of HPV 58-positive specimens, around 33% of HPV 16-positive specimens, and only one HPV18-positive specimen expressed L1. Conclusions: We demonstrated that HPV-derived transcripts are HPV genotype-dependent. Especially, expression patterns of L1 gene expression might reflect HPV genotype-dependent patterns of carcinogenesis. © 2020 The Author(s).

Published
2020

Catalog

Books, media, physical & digital resources
See catalog results