1. Continual Conscious Bioluminescent Imaging in Freely Moving Mice.
- Author
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Diaz JA, Geard A, FitzPatrick LM, Delhove JMKM, Buckley SMK, Waddington SN, McKay TR, and Karda R
- Subjects
- Animals, Biosensing Techniques, Gene Order, Genetic Vectors genetics, Luciferases, Firefly genetics, Mice, Plasmids genetics, Transfection, Transgenes, Gene Expression, Genes, Reporter, Luminescent Measurements methods
- Abstract
In vivo bioluminescent imaging allows the detection of reporter gene expression in rodents in real time. Here we describe a novel technology whereby we can generate somatotransgenic rodents with the use of a viral vector carrying a luciferase transgene. We are able to achieve long term luciferase expression by a single injection of lentiviral or adeno-associated virus vectors to newborn mice. Further, we describe whole body bioluminescence imaging of conscious mice in a noninvasive manner, thus enforcing the 3R's (replacement, reduction, and refinement) of biomedical animal research.
- Published
- 2020
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