1. [The regulating effect of antisense-S-Oligo on TYR gene expression and melanin production of melanocytes].
- Author
-
Zhang YG, Hu QH, Wang XZ, Qi ZL, Lin XX, Fang JL, and Dai CC
- Subjects
- 3' Flanking Region genetics, 5' Flanking Region genetics, Endothelins pharmacology, Melanocytes drug effects, Melanocytes metabolism, Melanocytes radiation effects, Oligodeoxyribonucleotides, Antisense genetics, Tyrosine metabolism, Ultraviolet Rays, Gene Expression, Melanins biosynthesis, Oligodeoxyribonucleotides, Antisense pharmacology, Tyrosine genetics
- Abstract
Objective: Despite the causes for melanin increase, the increased gene expression of TYR is a common pathological process. Based on this viewpoint, antisense-S-Oligo of TYR was designed and synthesized to regulate synthesis of melanin in order to explore the treatment for skin pigmentation., Methods: The cultured melanocytes were divided into 3 groups. The group 1 was treated with endothelin, group 2 treated with ultraviolet ray and group 3 was used as the control. In each group, the 5' antisense-S-Oligo, the 3' antisense-S-Oligo, the mixed antisense-S-Oligo of TYR or Dotap only was added. The melanin content and TYR gene expressions were examined., Results: The 5' antisense-S-Oligo, the 3' antisense-S-Oligo and the mixed antisense-S-Oligo significantly inhibited the increase of melanin content and TYR gene expression, which were caused by endothelin or ultraviolet ray treatment. Of the three treatments, the 3' antisense-S-Oligo showed the strongest effect., Conclusion: Antisense-S-Oligo has significant regulating effects on TYR gene expression and melanin content. The 3' antisense-S-Oligo is more effective than the 5' antisense-S-Oligo.
- Published
- 2003