8 results on '"Wrenger, Sabine"'
Search Results
2. Human PBMCs Form Lipid Droplets in Response to Spike Proteins.
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Sivaraman, Kokilavani, Pino, Paco, Raussin, Guillaume, Anchisi, Stephanie, Metayer, Charles, Dagany, Nicolas, Held, Julia, Wrenger, Sabine, Welte, Tobias, Wurm, Maria J., Wurm, Florian M., Olejnicka, Beata, and Janciauskiene, Sabina
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MONONUCLEAR leukocytes ,ENDOTOXINS ,STAINS & staining (Microscopy) ,LIPIDS ,LIPID metabolism ,GENE expression - Abstract
Intracellular lipid droplets (LDs) can accumulate in response to inflammation, metabolic stresses, and other physiological/pathological processes. Herein, we investigated whether spike proteins of SARS-CoV-2 induce LDs in human peripheral blood mononuclear cells (PBMCs) and in pulmonary microvascular endothelial cells (HPMECs). PBMCs or HPMECs were incubated alone or with endotoxin-free recombinant variants of trimeric spike glycoproteins (Alpha, Beta, Delta, and Omicron, 12 µg/mL). Afterward, cells were stained with Oil Red O for LDs, cytokine release was determined through ELISA, and the gene expression was analyzed through real-time PCR using TaqMan assays. Our data show that spikes induce LDs in PBMCs but not in HPMECs. In line with this, in PBMCs, spike proteins lower the expression of genes involving lipid metabolism and LD formation, such as SREBF1, HMGCS1, LDLR, and CD36. On the other hand, PBMCs exposed to spikes for 6 or 18 h did not increase in IL-1β, IL-6, IL-8, MCP-1, and TNFα release or expression as compared to non-treated controls. Thus, spike-induced LD formation in PBMCs seems to not be related to cell inflammatory activation. Further detailed studies are warranted to investigate in which specific immune cells spikes induce LDs, and what are the pathophysiological mechanisms and consequences of this induction in vivo. [ABSTRACT FROM AUTHOR]
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- 2023
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3. Mice inflammatory responses to inhaled aerosolized LPS: effects of various forms of human alpha1-antitrypsin.
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Sivaraman, Kokilavani, Wrenger, Sabine, Liu, Bin, Schaudien, Dirk, Hesse, Christina, Gomez-Mariano, Gema, Perez-Luz, Sara, Sewald, Katherina, DeLuca, David, Wurm, Maria J, Pino, Paco, Welte, Tobias, Martinez-Delgado, Beatriz, and Janciauskiene, Sabina
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PULMONARY alveolar proteinosis ,GRANULOCYTE-macrophage colony-stimulating factor ,PEPTIDES ,NITRIC-oxide synthases ,INFLAMMATION ,GENE expression - Abstract
Rodent models of lipopolysaccharide (LPS)–induced pulmonary inflammation are used for anti-inflammatory drug testing. We aimed to characterize mice responses to aerosolized LPS alone or with intraperitoneal (i.p.) delivery of alpha1-antitrypsin (AAT). Balb/c mice were exposed to clean air or aerosolized LPS (0.21 mg/mL) for 10 min per day, for 3 d. One hour after each challenge, animals were treated i.p. with saline or with (4 mg/kg body weight) one of the AAT preparations: native (AAT), oxidized (oxAAT), recombinant (recAAT), or peptide of AAT (C-36). Experiments were terminated 6 h after the last dose of AATs. Transcriptome data of mice lungs exposed to clean air versus LPS revealed 656 differentially expressed genes and 155 significant gene ontology terms, including neutrophil migration and toll-like receptor signaling pathways. Concordantly, mice inhaling LPS showed higher bronchoalveolar lavage fluid neutrophil counts and levels of myeloperoxidase, inducible nitric oxide synthase, IL-1β, TNFα, KC, IL-6, and granulocyte-macrophage colony-stimulating factor (GM-CSF). Plasma inflammatory markers did not increase. After i.p. application of AATs, about 1% to 2% of proteins reached the lungs but, except for GM-CSF, none of the proteins significantly influenced inflammatory markers. All AATs and C-36 significantly inhibited LPS-induced GM-CSF release. Surprisingly, only oxAAT decreased the expression of several LPS-induced inflammatory genes, such as Cxcl3 , Cd14 , Il1b , Nfkb1 , and Nfkb2 , in lung tissues. According to lung transcriptome data, oxAAT mostly affected genes related to transcriptional regulation while native AAT or recAAT affected genes of inflammatory pathways. Hence, we present a feasible mice model of local lung inflammation induced via aerosolized LPS that can be useful for systemic drug testing. [ABSTRACT FROM AUTHOR]
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- 2023
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4. Loss of Serpina1 in Mice Leads to Altered Gene Expression in Inflammatory and Metabolic Pathways.
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Meghadri, Sri Harsha, Martinez-Delgado, Beatriz, Ostermann, Lena, Gomez-Mariano, Gema, Perez-Luz, Sara, Tumpara, Srinu, Wrenger, Sabine, DeLuca, David S., Maus, Ulrich A., Welte, Tobias, and Janciauskiene, Sabina
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CHOLESTEROL metabolism ,GENE expression ,CHRONIC obstructive pulmonary disease ,KNOCKOUT mice ,RNA sequencing ,MICE - Abstract
The SERPINA1 gene encodes alpha1-antitrypsin (AAT), an acute phase glycoprotein and serine protease inhibitor that is mainly (80–90%) produced in the liver. Point mutations in the SERPINA1 gene can lead to the misfolding, intracellular accumulation, and deficiency of circulating AAT protein, increasing the risk of developing chronic liver diseases or chronic obstructive pulmonary disease. Currently, siRNA technology can knock down the SERPINA1 gene and limit defective AAT production. How this latter affects other liver genes is unknown. Livers were taken from age- and sex-matched C57BL/6 wild-type (WT) and Serpina1 knockout mice (KO) aged from 8 to 14 weeks, all lacking the five serpin A1a-e paralogues. Total RNA was isolated and RNA sequencing, and transcriptome analysis was performed. The knockout of the Serpina1 gene in mice changed inflammatory, lipid metabolism, and cholesterol metabolism-related gene expression in the liver. Independent single-cell sequencing data of WT mice verified the involvement of Serpina1 in cholesterol metabolism. Our results from mice livers suggested that designing therapeutic strategies for the knockout of the SERPINA1 gene in humans must account for potential perturbations of key metabolic pathways and consequent mitigation of side effects. [ABSTRACT FROM AUTHOR]
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- 2022
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5. Acute-Phase Protein α1-Antitrypsin--A Novel Regulator of Angiopoietin-like Protein 4 Transcription and Secretion.
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Frenzel, Eileen, Wrenger, Sabine, Immenschuh, Stephan, Koczulla, Rembert, Mahadeva, Ravi, Deeg, H. Joachim, Dinarello, Charles A., Welte, Tobias, Marcondes, A. Mario Q., and Janciauskiene, Sabina
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ALPHA 1-antitrypsin , *ANGIOPOIETIN-like proteins , *ENDOTHELIAL cells , *GENETIC transcription , *GENE expression - Abstract
The angiopoietin-like protein 4 (angptl4, also known as peroxisome proliferator-activated receptor [PPAR]γ-induced angiopoietin-related protein) is a multifunctional protein associated with acute-phase response. The mechanisms accounting for the increase in angptl4 expression are largely unknown. This study shows that human a1-antitrypsin (A1AT) upregulates expression and release of angplt4 in human blood adherent mononuclear cells and in primary human lung microvascular endothelial cells in a concentration- and time-dependent manner. Mononuclear cells treated for 1 h with A1AT (from 0.1 to 4 mg/ml) increased mRNA of angptl4 from 2- to 174-fold, respectively, relative to controls. In endothelial cells, the maximal effect on angptl4 expression was achieved at 8 h with 2 mg/ml A1AT (11-fold induction versus controls). In 10 emphysema patients receiving A1AT therapy (Prolastin), plasma angptl4 levels were higher relative to patients without therapy (nanograms per milliliter, mean [95% confidence interval] 127.1 [99.5-154.6] versus 76.8 [54.8-98.8], respectively, p = 0.045) and correlated with A1AT levels. The effect of A1AT on angptl4 expression was significantly diminished in cells pretreated with a specific inhibitor of ERK1/2 activation (UO126), irreversible and selective PPARγ antagonist (GW9662), or genistein, a ligand for PPARγ. GW9662 did not alter the ability of A1AT to induce ERK1/2 phosphorylation, suggesting that PPARγ is a critical mediator in the A1AT-driven angptl4 expression. In contrast, the forced accumulation of HIF-1a, an upregulator of angptl4 expression, enhanced the effect of A1AT. Thus, acute-phase protein A1AT is a physiological regulator of angptl4, another acute-phase protein. [ABSTRACT FROM AUTHOR]
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- 2014
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6. Krüppel-like zinc finger proteins in end-stage COPD lungs with and without severe alpha1-antitrypsin deficiency.
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Koczulla, A-Rembert, Jonigk, Danny, Wolf, Thomas, Herr, Christian, Noeske, Sarah, Klepetko, Walter, Vogelmeier, Claus, von Neuhoff, Nils, Rische, Johanna, Wrenger, Sabine, Golpon, Heiko, Voswinckel, Robert, Luisetti, Maurizio, Ferrarotti, Ilaria, Welte, Tobias, and Janciauskiene, Sabina
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OBSTRUCTIVE lung diseases ,ZINC-finger proteins ,GENE expression ,GENETIC regulation ,LIVER diseases - Abstract
Background: Chronic obstructive pulmonary disease (COPD) is influenced by environmental and genetic factors. An important fraction of COPD cases harbor a major genetic determinant, inherited ZZ (Glu342Lys) α1-antitrypsin deficiency (AATD). A study was undertaken to investigate gene expression patterns in end-stage COPD lungs from patients with and without AATD. Methods: Explanted lungs of end-stage ZZ AATD-related (treated and non-treated with AAT augmentation therapy) and "normal" MM COPD, and liver biopsies from patients suffering from liver cirrhosis with and without ZZ AATD were used for gene expression analysis by Affymetrix microarrays or RT-PCR. Results: A total of 162 genes were found to be differentially expressed (p-value ≤ 0.05 and |FC| ≥ 2) between MM and ZZ COPD patients. Of those, 134 gene sets were up-regulated and 28 were down-regulated in ZZ relative to MM lung tissue. A subgroup of genes, zinc finger protein 165, snail homolog 1 (Drosophila) (SNAI1), and Kruppel-like transcription factors (KLFs) 4 (gut), 9 and 10, perfectly segregated ZZ and MM COPD patients. The higher expression of KLF 9 and KLF10 has been verified in the replication cohort with AATD-related end-stage lung emphysema and liver cirrhosis. Furthermore, higher expression of KLF9, SNAI1 and DEFA1 was found in ZZ COPD lungs without augmentation therapy relative to MM COPD or ZZ COPD with augmentation therapy. Conclusions: These results reveal the involvement of transcriptional regulators of the zinc-finger family in COPD pathogenesis and provide deeper insight into the pathophysiological mechanisms of COPD with and without AATD. [ABSTRACT FROM AUTHOR]
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- 2012
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7. Ex vivo study on the human blood neutrophil circadian features and effects of alpha1-antitrypsin and lipopolysaccharide.
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Held, Julia, Sivaraman, Kokilavani, Wrenger, Sabine, Si, Wenzhang, Welte, Tobias, Immenschuh, Stephan, and Janciauskiene, Sabina
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MYELOID cells , *GENE expression , *MOLECULAR clock , *PHAGOCYTOSIS , *NEUTROPHILS , *CIRCADIAN rhythms - Abstract
Neutrophils perform various functions in a circadian-dependent manner; therefore, we investigated here whether the effect of alpha1-antitrypsin (AAT), used as augmentation therapy, is dependent on the neutrophil circadian clock. AAT is a vital regulator of neutrophil functions, and its qualitative and/or quantitative defects have significant implications for the development of respiratory diseases. Whole blood from 12 healthy women age years, mean (SD) 29.92 (5.48) was collected twice daily, 8 h apart, and incubated for 30 min at 37 °C alone or with additions of 2 mg/ml AAT (Respreeza) and/or 5 μg/ml lipopolysaccharide (LPS) from Escherichia coli. Neutrophils were then isolated to examine gene expression, migration and phagocytosis. The expression of CD14 , CD16 , CXCR2 and SELL (encoding CD62L) genes was significantly higher while CDKN1A lower in the afternoon than in the morning neutrophils from untreated blood. Neutrophils isolated in the afternoon had higher migratory and phagocytic activity. Morning neutrophils isolated from AAT-pretreated blood showed higher expression of CXCR2 and SELL than those from untreated morning blood. Pretreatment of blood with AAT enhanced migratory properties of morning but not afternoon neutrophils. Of all genes analysed, only CXCL8 expression was strongly upregulated in morning and afternoon neutrophils isolated from LPS-pretreated blood, whereas CXCR2 expression was downregulated in afternoon neutrophils. The addition of AAT did not reverse the effects of LPS. The circadian clock of myeloid cells may affect the effectiveness of various therapies, including AAT therapy used to treat patients with AAT deficiency, and needs further investigation. [Display omitted] [ABSTRACT FROM AUTHOR]
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- 2024
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8. Polymerization of misfolded Z alpha-1 antitrypsin protein lowers CX3CR1 expression in human PBMCs.
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Tumpara, Srinu, Ballmaier, Matthias, Wrenger, Sabine, König, Mandy, Lehmann, Matthias, Lichtinghagen, Ralf, Martinez-Delgado, Beatriz, Korenbaum, Elena, DeLuca, David, Jedicke, Nils, Welte, Tobias, Fromme, Malin, Strnad, Pavel, Stolk, Jan, and Janciauskiene, Sabina
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TRYPSIN , *MONONUCLEAR leukocytes , *PROTEIN folding , *TRYPSIN inhibitors , *GENE expression , *CHEMOKINE receptors - Abstract
Expression levels of CX3CR1 (C-X3-C motif chemokine receptor 1) on immune cells have significant importance in maintaining tissue homeostasis under physiological and pathological conditions. The factors implicated in the regulation of CX3CR1 and its specific ligand CX3CL1 (fractalkine) expression remain largely unknown. Recent studies provide evidence that host's misfolded proteins occurring in the forms of polymers or amyloid fibrils can regulate CX3CR1 expression. Herein, a novel example demonstrates that polymers of human ZZ alpha-1 antitrypsin (Z-AAT) protein, resulting from its conformational misfolding due to the Z (Glu342Lys) mutation in SERPINA1 gene, strongly lower CX3CR1 mRNA expression in human peripheral blood mononuclear cells (PBMCs). This parallels with increase of intracellular levels of CX3CR1 and Z-AAT proteins. Presented data indicate the involvement of the CX3CR1 pathway in the Z-AAT-related disorders and further support the role of misfolded proteins in CX3CR1 regulation. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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