1. [Expression characteristics of the Daxx gene in the mouse testis during spermatogenesis].
- Author
-
Zhang Z, Deng Q, Wu Y, Huang XB, Yao L, Jiang ZM, and Gui YT
- Subjects
- Animals, Carrier Proteins metabolism, Cell Nucleus genetics, Cell Nucleus metabolism, Co-Repressor Proteins, Intracellular Signaling Peptides and Proteins metabolism, Male, Mice, Mice, Knockout, Molecular Chaperones, Nuclear Proteins metabolism, Receptors, Androgen genetics, Sertoli Cells, Carrier Proteins genetics, Gene Expression, Intracellular Signaling Peptides and Proteins genetics, Nuclear Proteins genetics, Spermatogenesis genetics, Testis metabolism
- Abstract
Objective: To investigate the expression characteristic of the Daxx gene in the mouse testis and its role in spermatogenesis., Methods: Real-time PCR, Western blot and immunofluorescence were used in examining the expression characteristics of DAXX in the testis tissue from wild-type, Sertoli cell-specific androgen receptor knockout (SCARKO) and androgen receptor knockout (ARKO) mice at different postnatal weeks ., Results: The Daxx gene was highly expressed in the testis tissue and mainly in the nuclei of the wild-type mice at 4 postnatal weeks. Compared with the wild-type, the ARKO mice showed a markedly decreased expression of DAXX (0.299±0.026), which displayed a polar distribution in the spermatogenic cells (0.853±0.058) and exhibited no significant difference in the SCARKO mice (1.000±0.015)., Conclusions: The Daxx gene expression is the highest in the middle-stage development of the mouse testis, significantly decreased in ARKO mice as compared with the wild-type, and its location influenced by specific AR knockout in Sertoli cells. DAXX may be involved in the regulation of spermatogenesis in mice.
- Published
- 2017