1. TALEN-Based Knockout System.
- Author
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Yoshida K and Treen N
- Subjects
- Amino Acid Sequence, Animals, Ciona intestinalis growth & development, Ciona intestinalis ultrastructure, DNA, Recombinant administration & dosage, DNA, Recombinant genetics, Enhancer Elements, Genetic genetics, Genes, Reporter, Genetic Vectors genetics, Germ-Line Mutation, Glucosyltransferases deficiency, Glucosyltransferases genetics, In Situ Hybridization methods, Larva, Luminescent Proteins genetics, Microinjections methods, Mutagenesis, Organ Specificity genetics, Ovum, Plasmids genetics, Promoter Regions, Genetic genetics, RNA administration & dosage, RNA genetics, RNA, Messenger administration & dosage, RNA, Messenger genetics, Ciona intestinalis genetics, Gene Knockout Techniques, Transcription Activator-Like Effector Nucleases genetics
- Abstract
Targeted mutagenesis of genes-of-interest is a powerful method of addressing the functions of genes. Genome editing techniques, such as transcriptional activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 systems, have enabled this approach in various organisms because of their ease of use. In the ascidian, Ciona intestinalis, recent studies show that TALEN-based knockout can be applied to establishing both mutant lines and tissue-specific knockout for addressing gene functions. Here, we introduce recent updates to the TALEN toolkit that facilitate detailed functional analysis of genes in ascidians.
- Published
- 2018
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