6 results on '"Marina Popova"'
Search Results
2. CO Sense and Release Flavonols: Progress toward the Development of an Analyte Replacement PhotoCORM for Use in Living Cells
- Author
-
Tatiana Soboleva, Abby D. Benninghoff, Marina Popova, Lisa M. Berreau, and Elsevier Ltd
- Subjects
chemistry.chemical_classification ,Analyte ,PhotoCORM ,General Chemical Engineering ,Co detection ,General Chemistry ,Fluorescence ,flavonols ,Article ,In vitro ,carbon monoxide ,lcsh:Chemistry ,chemistry.chemical_compound ,Chemistry ,Flavonols ,chemistry ,lcsh:QD1-999 ,analyte replacement ,Biophysics ,Molecule ,Co release ,Carbon monoxide - Abstract
Carbon monoxide (CO) is a signaling molecule in humans. Prior research suggests that therapeutic levels of CO can have beneficial effects in treating a variety of physiological and pathological conditions. To facilitate understanding of the role of CO in biology, molecules that enable fluorescence detection of CO in living systems have emerged as an important class of chemical tools. A key unmet challenge in this field is the development of fluorescent analyte replacement probes that replenish the CO that is consumed during detection. Herein, we report the first examples of CO sense and release molecules that involve combining a common CO-sensing motif with a light-triggered CO-releasing flavonol scaffold. A notable advantage of the flavonol-based CO sense and release motif is that it is trackable via fluorescence in both its pre- and postsensing (pre-CO release) forms. In vitro studies revealed that the PdCl2 and Ru(II)-containing CORM-2 used in the CO sensing step can result in metal coordination to the flavonol, which minimizes the subsequent CO release reactivity. However, CO detection followed by CO release is demonstrated in living cells, indicating that a cellular environment mitigates the flavonol/metal interactions. more...
- Published
- 2020
Catalog
3. Visible-Light-Activated Quinolone Carbon-Monoxide-Releasing Molecule: Prodrug and Albumin-Assisted Delivery Enables Anticancer and Potent Anti-Inflammatory Effects
- Author
-
Tatiana Soboleva, Lisa M. Berreau, Suliman Ayad, Marina Popova, and Abby D. Benninghoff
- Subjects
Light ,Anti-Inflammatory Agents ,Serum albumin ,Antineoplastic Agents ,Plasma protein binding ,Quinolones ,010402 general chemistry ,01 natural sciences ,Biochemistry ,Article ,Catalysis ,Mice ,chemistry.chemical_compound ,Colloid and Surface Chemistry ,Human Umbilical Vein Endothelial Cells ,Fluorescence microscope ,Animals ,Humans ,Prodrugs ,Cytotoxicity ,A549 cell ,Carbon Monoxide ,biology ,010405 organic chemistry ,Serum Albumin, Bovine ,General Chemistry ,Prodrug ,Combinatorial chemistry ,0104 chemical sciences ,RAW 264.7 Cells ,chemistry ,A549 Cells ,Cancer cell ,biology.protein ,Cattle ,Protein Binding ,Carbon monoxide - Abstract
The delivery of controlled amounts of carbon monoxide (CO) to biological targets is of significant current interest. Very few CO-releasing compounds are currently known that can be rigorously controlled in terms of the location and amount of CO released. To address this deficiency, we report herein a new metal-free, visible light-induced CO-releasing molecule (photoCORM) and its prodrug oxidized form, which offer new approaches to controlled, localized CO-delivery. The new photoCORM, based on a 3-hydroxybenzo[g]quinolone framework, releases one equivalent of CO upon visible light illumination under a variety of biologically-relevant conditions. This non-toxic compound can be tracked prior to CO release using fluorescence microscopy and produces a non-toxic byproduct following CO release. An oxidized prodrug form of the photoCORM is reduced by cellular thiols, providing an approach toward activation in the reducing environment of cancer cells. Strong non-covalent affinity of the non-metal photoCORM to albumin enables use of an albumin:photoCORM complex for targeted CO delivery to cancer cells. This approach produced cytotoxicity IC(50) values among the lowest reported to date for CO delivery to cancer cells by a photoCORM. This albumin:photoCORM complex is also the first CO delivery system to produce significant anti-inflammatory effects when introduced at nanomolar photoCORM concentration. more...
- Published
- 2018
- Full Text
- View/download PDF
4. Properties of a flavonol-based photoCORM in aqueous buffered solutions: influence of metal ions, surfactants and proteins on visible light-induced CO release
- Author
-
Lisa M. Berreau, Tatiana Soboleva, Atta M. Arif, and Marina Popova
- Subjects
Tris ,Aqueous solution ,biology ,010405 organic chemistry ,General Chemical Engineering ,Metal ions in aqueous solution ,Inorganic chemistry ,Serum albumin ,General Chemistry ,Morin ,010402 general chemistry ,Human serum albumin ,01 natural sciences ,Binding constant ,0104 chemical sciences ,chemistry.chemical_compound ,chemistry ,biology.protein ,medicine ,Bovine serum albumin ,medicine.drug - Abstract
The properties of the extended flavonol 3-hydroxy-2-phenyl-benzo[g]chromen-4-one (2a) in DMSO : aqueous buffer solutions at pH = 7.4, including in the presence of metal ions, surfactants and serum albumin proteins, have been examined. Absorption and emission spectral studies of 2a in 1 : 1 DMSO : PBS buffer (pH = 7.4) indicate that a mixture of neutral and anionic forms of the flavonol are present. Notably, in 1 : 1 DMSO : TRIS buffer (pH = 7.4) only the neutral form of the flavonol is present. These results indicate that the nature of the buffer influences the acid/base equilibrium properties of 2a. Introduction of a Zn(II) complex of 2a− to a 1 : 1 DMSO : aqueous buffer (TRIS or PBS, pH = 7.4) solution produces absorption and emission spectral features consistent with the presence of a mixture of neutral 2a along with Zn(II)-coordinated or free 2a−. The nature of the anionic species present depends on the buffer composition. PBS buffered solutions (pH = 7.4) containing the surfactants CTAB or SDS enable 2a to be solubilized at a much lower percentage of DMSO (3.3–4.0%). Solutions containing the cationic surfactant CTAB include a mixture of 2a and 2a− whereas only the neutral flavonol is present in SDS-containing buffered solution. Compound 2a is also solubilized in TRIS buffer solutions at low cocentrations of DMSO (3.3%, pH = 7.4) in the presence of serum albumin proteins. Stern–Volmer analysis of the quenching of the inherent protein fluorescence indicates static binding of 2a to the proteins. The binding constant for this interaction is lower than that found for naturally-occurring flavonols (quercetin or morin) or 3-hydroxyflavone. Compound 2a binds to Site I of bovine and human serum albumin proteins as indicated by competition studies with warfarin and ibuprofen, as well as by docking investigations. The quantum yield for CO release from 2a (λirr = 419 nm) under aqueous conditions ranges from 0.0006(3) when the compound is bound to bovine serum albumin to 0.017(1) when present as a zinc complex in a 1 : 1 DMSO : H2O solution. Overall, the results of these studies demonstrate that 2a is a predictable visible light-induced CO release compound under a variety of aqueous conditions, including in the presence of proteins. more...
- Published
- 2017
- Full Text
- View/download PDF
5. Preparation of graphene oxide/dendrimer hybrid carriers for delivery of doxorubicin
- Author
-
Marina Popova, Toyoko Imae, Hong Boon Lee, Ampornphan Siriviriyanun, Lik Voon Kiew, Chung Yeng Looi, Won Fen Wong, and Lip Yong Chung
- Subjects
biology ,Chemistry ,General Chemical Engineering ,General Chemistry ,biology.organism_classification ,Industrial and Manufacturing Engineering ,In vitro ,HeLa ,Biochemistry ,Dendrimer ,Biophysics ,medicine ,Environmental Chemistry ,Doxorubicin ,Viability assay ,Nanocarriers ,Hybrid material ,Drug carrier ,medicine.drug - Abstract
Hybrid carriers were successfully synthesized by chemically binding OH-terminated fourth generation poly(amido amine) dendrimer and folic acid on graphene oxide (GO) with a size of 100 nm, and their drug carrier ability was compared with hybrids of 1500 nm GO. The small hybrid carriers possessed a 1.3 times lower loading capacity (1.43 g/g(GO)) for doxorubicin (DOX) than the large hybrid carriers. However, the amount (86%) of DOX released at pH 5.5 from the small hybrids after 72 h was 1.14 times larger than from the large hybrids. This difference could be due to the extent of π–π interaction between DOX and the graphitic domain on GO. In vitro MTT assays revealed that the small hybrids led to higher cell viability than 63% at 0.75 μg/ml in HeLa human cervical cancer cells. An investigation of the intracellular uptake of hybrid carriers revealed the efficient internalization of small hybrids in the HeLa cells (97% at 62.5 mg/ml). Taken together, these results indicate that the small hybrids consisting of 100 nm GO carrier can successfully load and deliver the anticancer drug DOX into HeLa cells. In the carriers, dendrimer had a role of stable suspension of the hybrids in water, and folic acid acted as an effective anchoring of the hybrids on HeLa cells. The present investigation highlights the potential use of these hybrid materials as nanocarriers with high loading and releasing efficiency for the delivery of anticancer drugs to tumor cells. more...
- Published
- 2015
- Full Text
- View/download PDF
6. Electrodeposited nickel-sulfide films as competent hydrogen evolution catalysts in neutral water
- Author
-
Sheraz Gul, Yujie Sun, Marina Popova, Junko Yano, Nan Jiang, Lia Bogoev, and Royal Society of Chemistry
- Subjects
Tafel equation ,Nickel sulfide ,Absorption spectroscopy ,Renewable Energy, Sustainability and the Environment ,Chemistry ,Electro-deposited nickel ,Hydrogen evolution ,Neutral water ,Sulfide films ,Inorganic chemistry ,General Chemistry ,Catalysis ,chemistry.chemical_compound ,X-ray photoelectron spectroscopy ,General Materials Science ,Bulk electrolysis ,Faraday efficiency ,Deposition (law) - Abstract
The development of low-cost, efficient, and robust electrocatalysts of the hydrogen evolution reaction (HER) is a crucial step toward the conversion and storage of sustainable and carbon-neutral energy resources, such as solar energy. Not only the HER catalysts need to be composed of inexpensive elements, they are also desirable to be prepared at low energy cost. In this work, we report that nickel-sulfide (Ni-S) films prepared by facile potentiodynamic deposition are active HER catalysts in aqueous media. Notably, the Ni-S films showed catalytic activity in water with a wide range of pH values (0 to 14), as well as in natural water. In pH 7 phosphate buffer, a current density of 60 mA cm-2 could be achieved with a Tafel slope of 77 mV dec-1 and a Faradaic efficiency of 100%. A long-term bulk electrolysis of the Ni-S film exhibited steady current over 100 h with no deactivation, demonstrating its superior stability in neutral water. Further, an initial activation process was observed, which is likely due to the increase in the effective surface area of the Ni-S film under electrocatalytic conditions. A suite of characterization techniques, including X-ray photoelectron spectroscopy and X-ray absorption spectroscopy, were conducted to probe the composition and structure of the Ni-S film, revealing that its major component is Ni3S2 which was preserved under electrocatalytic conditions. more...
- Published
- 2014
- Full Text
- View/download PDF
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.