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Introduction: The incidence of colorectal cancer is steadily increasing, and the detection of related molecular targets is critical for its diagnosis and treatment. Long noncoding RNA (lncRNA) can play a regulatory role before and after genome transcription, and epigenetic regulation is involved in the process of tumorigenesis and tumor development. Method: In this study, qRT-PCR was performed to evaluate the expression of AK093407 in colon cancer and colon para-carcinoma tissues and HCT-15 and HCT-116 cells. SiRNA was transfected into HCT-15 and HCT-116 cells to knock down lncRNA-AK093407. Then, MTT assay was used to test cell proliferation, and flow cytometry was used to test apoptosis and cell cycle. The protein expression of caspase-3, caspase-8, caspase-9, bax, bcl-2, cyclin-A1, cyclin-B1, cyclin-D1, cyclin- E1, p21, p27, and p-Stat3 was determined by Western blot. Results: The results showed that the expression of AK093407 in human colon cancer tissue was higher than in para-carcinoma tissue. The amount of AK093407 in HCT-15 and HCT-116 cells was higher than that in normal colorectal epithelial NM460 cells. When AK093407 was silenced, the proliferation of HCT-15 and HCT-116 cells decreased, the apoptosis rate increased, the cell cycle was arrested in the G1/S phase, the expression of caspase-3, caspase-8, caspase-9, bax, cyclin-A1, cyclin- B1, p21, p27 increased, and the expression of bcl-2, cyclin-D1, cyclin-E1, p-Stat3 decreased. Conclusion: These results showed that knockdown of AK093407 could inhibit colon cancer cell proliferation, induce apoptosis and cell cycle arrest, influence the expression of vital factors in mitochondrial apoptosis pathway and cell cycle regulatory pathway, and may negatively regulate JAK/STAT3 through down-regulating p-Stat3.

To evaluate the reliability and validity of the Chinese version of the eight-item Morisky Medication Adherence Scale (MMAS-8) in Chinese patients with systemic lupus erythematosus (SLE).The survey was conducted with a consecutive sampling of 158 Chinese SLE patients attending public hospitals from January to March 2021. We used the translated Chinese version of the MMAS-8 to collect related data. Reliability, item, and factor analyses were used to test the reliability and validity of the MMAS-8 scale in the selected patients. The internal consistency reliability was evaluated using Cronbach's α coefficient. Test-retest reliability was assessed using intraclass correlation coefficients (ICCs) in a subset of 30 participants. Construct validity was evaluated using confirmatory factor analysis and correlations between the Self-efficacy for Appropriate Medication Use Scale (SEAMS) and related measures.The internal consistency reliability of the Chinese version of the MMAS-8 was high (Cronbach's α = 0.817), and the test-retest reliability was excellent (intraclass correlation = 0.947; P 0.001). There were significant differences in the F test and t test between the two extreme groups before and after the ranking of 27% of the questionnaire scores (P 0.001). The Kaiser-Meyer-Olkin (KMO) value of construct validity was 0.860. The spherical test value of Bartlettgers was 417.8822. Factor analysis yielded three components that accounted for 69.375% of the total variance. Exploratory factor analysis identified three dimensions of the Chinese version of the MMAS-8. In terms of criterion validity, the correlation of the MMAS-8 score in SEAMS indicated that the convergent validity was good (r = 0.926; P 0.001).This study shows that the Chinese version of the Medication Adherence Scale-8 is a reliable and valid tool for assessing medication adherence in Chinese SLE patients. Key Points • Many factors affect medication adherence in SLE patients. • Many questionnaires measure medication adherence levels. • There is a lack of reliable validation of medication adherence questionnaires specifically for SLE patients.

Purpose To determine the effect of low-intensity ultrasound on cancer cell proliferation in vitro and tumor growth in vivo. Methods In vitro, several cancer cell lines were exposed to low-intensity ultrasound at 0.11 W/cm2 for 2 min. Of the cell lines screened, melanoma C32 is one of the cell lines that showed sensitivity to growth inhibition by ultrasound and was therefore used in succeeding experiments. In vivo, under the same ultrasound conditions used in vitro, C32 tumors in mice were exposed to ultrasound daily for 2 weeks, and the tumor volumes were monitored weekly using sonography. Results In vitro, C32 cell growth was inhibited, attaining 43.2% inhibition on the 3rd day. In vivo, tumor growth was significantly inhibited, with the treated tumors exhibiting 2.7-fold slowed tumor growth vs. untreated tumors at week 2. Such inhibition was not associated with increased cell death. Several genes related to the cell cycle and proliferation were among those significantly regulated. Conclusion These findings highlight the potential of low-intensity ultrasound to inhibit tumor growth in a noninvasive, safe, and easy-to-administer way. In addition, this may suggest that the mechanical stress induced by ultrasound on C32 cells may have affected the intrinsic biomolecular mechanism related to the cell growth of this particular cell line. Further research is needed to identify which of the regulated genes played key roles in growth inhibition.

The first copper-catalyzed regiodivergent cyanoboration of internal allenes with B

Song Wang,1,* Rui-Chen Gao,2,* Rui Li,3,* Guo-Cui Wu1 1Lianhua Community Health Service Center, The Second Affiliated Hospital, Anhui Medical University, Hefei, Anhui, People’s Republic of China; 2School of Nursing, Anhui Medical University, Hefei, Anhui, People’s Republic of China; 3Department of Hospital Infection Management, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, People’s Republic of China*These authors contributed equally to this workCorrespondence: Guo-Cui WuSchool of Nursing, Anhui Medical University, 15 Feicui Road, Hefei, 230601, Anhui, People’s Republic of ChinaEmail gcwu82@126.comObjective: To investigate the mental illness and correlated factors of primary medical staff during the COVID-19 outbreak in Hefei city, China.Methods: A total of 180 primary medical staff were randomly selected from seven community hospitals in Hefei Economic and Technological Development Zone as a study group. One hundred and eighty-two health people were recruited as the control group. The self-rating Anxiety Scale (SAS), self-rating Depression Scale (SDS) and Psychological questionnaire of public health emergencies were distributed to them for evaluation.Results: The score of SAS, SDS in study group was higher than that in control group [(35.57± 10.39) vs (31.31± 7.98); (44.16± 8.46) vs (41.47± 9.47)] (t=4.371, P< 0.001; t=2.849, P=0.005). The fear subscale and total score in the psychological questionnaire of sudden public health events were negatively correlated with age (r=− 0.216, P=0.004; r=− 0.154, P=0.039). Marriage was negatively correlated with depression subscales in psychological questionnaires of SAS, SDS and sudden public health events (r=− 0.184, P=0.013; r=− 0.298, P< 0.001; r=− 0.161, P=0.031; r=− 0.147, P=0.049). Education level was positively correlated with the total score of a psychological questionnaire for sudden public health events (r=0.151, P=0.043); Logistic regression analysis showed that marital status was a protective factor of psychological abnormality.Conclusion: It is necessary to pay attention to the psychological status of primary medical staff, especially the young unmarried medical staff.Keywords: novel coronavirus pneumonia, doctor, nurse, anxiety, depressed

To obtain a reliable estimation on the sleep quality in patients with systemic lupus erythematosus (SLE) and identify the main sleep problems, a meta-analysis was performed.Up to March 21, 2020, PubMed, EMBASE, and Cochrane Library were searched; quality evaluation were conducted with Newcastle-Ottawa Scale; statistical analyses were performed by stata14.0 software; results were expressed by weighted mean difference or standardized mean difference (WMD/SMD) and 95% confidence interval (CI).Eighteen case-control studies were included in meta-analysis, 1086 SLE patients and 2866 controls were collected. The score of sleep quality in the case group was higher than that in the control group (SMD = 1.03, 95% CI: 0.80-1.27), and so was the Pittsburgh Sleep Quality Index (PSQI) (WMD = 3.45, 95% CI: 2.49-4.42). The first three complaints of sleep problems in PSQI were daytime dysfunction (WMD = 0.64, 95% CI: 0.36-0.92), subjective sleep quality (WMD = 0.62, 95% CI: 0.40-0.84), and habitual sleep efficiency (WMD = 0.54, 95% CI: 0.37-0.72). Subgroup analyses showed that the score of sleep quality in SLE patients were higher than controls among different regions, races, and disease duration. The sleep quality score of SLE patients with fibromyalgia (FM) was higher than that in general control, but no significant difference as compared with SLE patients without FM.Our meta-analysis indicates that the sleep quality of SLE patients is worse than that of the general population; thus, more attention should be paid to the sleep status among this disease. Key Points •The sleep quality of SLE patients is worse than that of the general population. •Region, race, and disease duration are correlated with sleep quality in SLE patients.

Given the high global prevalence and mortality associated with gastric cancer, and its known causal link with Helicobacter pylori infection, it is important to have a biomarker to identify malignant transformation at early stages. Previously, we, and others, have reported that H. pylori-induced epigenetic changes could mediate carcinogenic transformation of the gastric cells. Also, CXCL1 secreted by gastric cancer cells was reported as a key diagnostic and prognostic biomarker for the pathogenic progression of gastric cancer. In this study, for the first time, we aimed to investigate the role of H. pylori-induced DNA methylation-based epigenetic regulation of CXCL1. In silico analysis of publicly available datasets and in vitro experiments were performed. Our results showed that CXCL1 is highly expressed in both gastric cancer tissues and gastric cancer cells infected with H. pylori. Further, we showed and confirmed that H. pylori-mediated overexpression of CXCL1 is due to hypomethylation of its promoter region. Since epigenetic events such as DNA methylation happen early in the sequence; H. pylori-induced CXCL1 hypomethylation could likely be detected at an early stage of gastric cancer development. Epigenetic modifications, such as CXCL1 hypomethylation, are reversible and could potentially be a therapeutic target using demethylation drugs.

Background Metabolic dysfunction-associated fatty liver disease (MAFLD) is a common global chronic liver disease. Jiuzhuan Huangjing Pills (JHP) have been used for the treatment of human disease for over a thousand years, but their efficacy and underlying mechanism(s) of action against MAFLD are unknown. We investigated the alleviating effects of JHP on high-fat diet (HFD)-induced MAFLD. Methods In vitro and in vivo methods were used to evaluate the effects of JHP on MAFLD. L02 adipocyte models were induced by fat emulsion and adipocytes were treated with JHP for 24 h. MAFLD rat models were induced by HFD-feeding and were intragastrically administered JHP for 12 weeks. Changes in fat accumulation, L02 cell damage, body weight, food intake, histological parameters, organ indexes, biochemical parameters, and mitochondrial indicators including ultrastructure, oxidative stress, energy metabolism, and fatty acid metabolism were investigated. Results JHP attenuated the increase in levels of total cholesterol, triglyceride, low density lipoprotein cholesterol, alanine transaminase, and aspartate transaminase levels, and significantly increased high density lipoprotein cholesterol. JHP up-regulated levels of glutathione (GSH) and superoxide dismutase (SOD), and down-regulated malondialdehyde (MDA). JHP afforded protection to the mitochondrial ultrastructure, and inhibited the HFD-induced increase in MDA and the reduction of SOD, GSH, ATP synthase, and complex I and II, in liver mitochondria. JHP regulated the expression of β-oxidation genes, including acyl-CoA dehydrogenase, cyl-CoA dehydrogenase long chain, carnitine palmitoyltransferase 1A, carnitine palmitoyltransferase 1B, peroxisomal proliferator-activated receptor-gamma coactivator-1α and peroxide proliferator activated receptor α. Conclusion JHP alleviates HFD-induced MAFLD through the protection of mitochondrial function.

Previous studies concerning the circulating interleukin-17 (IL-17) in systemic lupus erythematosus (SLE) were contradictory.To further precisely investigate circulating IL-17 in SLE and evaluate its influential factors by meta-analysis.EMBASE, PubMed and Cochrane Library were comprehensively searched to obtain studies on circulating IL-17 in SLE patients by November 22, 2018. The results were illustrated by pooled standard mean difference (SMD) with corresponding 95% confidence interval (CI) using random-effects model as there was significant heterogeneity, which was estimated using CochranOverall, 1872 articles were reviewed and 20 studies involving 1067 subjects with SLE and 721 healthy controls (HCs) were enrolled in the final analysis according to inclusion criteria. Compared with HCs, circulating IL-17 levels in SLE patients were elevated (SMD: 1.183, 95% CI: 0.763-1.603;SLE patients have higher circulating IL-17 levels, which is influenced by ethnic, age and disease duration, literature quality and measurements.

BACKGROUND To assess the non-adherence rate among pulmonary tuberculosis (TB) patients in Anhui Province, eastern China and to explore the influential factors, so as to identify targets for intervention. MATERIAL AND METHODS A total of 339 TB patients were recruited from TB dispensaries in 8 counties of Anhui Province, eastern China using a stratified sampling method. All study subjects were surveyed using a structured questionnaire. Differences between groups involving categorical data were analyzed using the chi-square test. RESULTS Overall, of the 339 patients, 33.63% missed medication. Divorced and widowed patients were more likely to miss medication compared with those who were married or unmarried (P0.01). Regarding the knowledge related to topics such as transmission route, preventive measures, and suspicious symptoms, the awareness rate in the group with good medication compliance was higher than in the group with poor compliance (P0.05). We found that compliance was not significantly associated with seeking medical treatment in professional institutions, the national free TB treatment policy, or discrimination (P0.05). The rate of non-compliance under supervision (26.10%) was lower than that without supervision (64.18%) (P0.001). CONCLUSIONS The anti-TB treatment non-adherence rate in TB patients is relatively high in Anhui Province, eastern China, and is associated with marital status, annual income, TB knowledge, and medical staff visits.

Background Due to varying degrees of difficulty in obtaining different mesenchymal stem cells (MSCs), the distinct pain levels and treatment costs, and for providing concrete evidence for future clinical practice, a thorough comparison of all relevant MSCs remained critical. Hence, this study aimed to achieve this objective to compare the efficacy of MSCs obtained from different sources in clinical outcomes and cartilage repair of knee osteoarthritis (KOA). Methods The EmBase, PubMed and Cochrane Library databases were searched for eligible studies. Randomized controlled trials (RCTs) that compared MSCs from different sources with placebo or each other in KOA patients. Conventional meta-analysis and frequentist network meta-analysis (NMA) were conducted. The primary clinical outcome was pain relief. The frequentist NMA was conducted using Stata with the "network" command. Results Eight studies (seven trials) involving 203 KOA patients were included in this meta-analysis. The MSCs were considered superior over placebo for pain relief and improved function in KOA, but showed no statistically significant differences for cartilage regeneration. Among all the MSCs, the adipose tissue-derived MSCs (AD-MSCs) most effectively relieved pain. Conclusion These findings suggested that MSCs are effective in the treating of KOA. AD-MSCs might be the most effective for relieving pain, and Umbilical cord-derived mesenchymal stem cells (UC-MSCs) might be the most effective for improving function. However, the current evidence does not support the use of MSCs for improving cartilage repair in KOA patients.

This study aimed to investigate the seroreactivity of Coronavirus disease 2019 (COVID-19) vaccination and its adverse events among systemic lupus erythematosus (SLE) patients, rheumatoid arthritis (RA) patients, and healthy controls (HCs).A total of 60 SLE patients, 70 RA patients and 35 HCs, who received a complete inactivated COVID-19 vaccine (Vero cells) regimen, were recruited in the current study. Serum IgG and IgM antibodies against Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) were determined by using chemiluminescent microparticle immunoassay (CMIA).There were no significant differences regarding the seroprevalences of IgG and IgM antibodies against SARS-CoV-2, and the self-reported vaccination-related adverse events among SLE patients, RA patients and HCs. The inactivated COVID-19 vaccines appeared to be well-tolerated and moderately immunogenic. In addition, case-only analysis indicated that in SLE patients, the disease manifestation of rash and anti-SSA autoantibody were associated with seroprevalence of IgG antibody against SARS-CoV-2, whereas the uses of ciclosporin and leflunomide had influence on the seroprevalence of IgM antibody against SARS-CoV-2. In RA patients, rheumatoid factor (RF) appeared to be associated with the seroprevalence of IgG antibody against SARS-CoV-2.Our study reveals that the seroprevalences of IgG and IgM antibodies against SARS-CoV-2 and vaccination-related adverse effects are similar among SLE, RA and HCs, suggesting that COVID-19 vaccine is safe and effective for SLE and RA patients to prevent from the pandemic of COVID-19.

Diabetic kidney disease (DKD) is the major cause of end-stage renal disease (ESRD). In the past few decades, there has been a large amount of evidence to highlight the pivotal role of oxidative stress in the development and progression of DKD. However, the detailed molecular mechanisms are not fully elucidated. A new sight has been established that the mitochondrial acetyltransferase GCN5L1 participates in cellular redox homeostasis maintenance in DKD. Firstly, we found that the expression of GCN5L1 is significantly elevated both in human and mouse kidney tissues with DKD and in hyperglycemic renal tubular epithelial cells (TECs), while deletion of GCN5L1 could effectively ameliorate oxidative stress-induced renal injury in DKD. Furthermore, deletion of GCN5L1 could reduce MnSOD acetylation on lysine 68 and activate its activity, thereby scavenging excessive ROS and relieving oxidative stress-induced renal inflammation and fibrosis. In general, GCN5L1-mediated acetylation of MnSOD exacerbated oxidative stress-induced renal injury, suggesting that GCN5L1 might be a potential intervention target in DKD.

Cadmium (Cd) is a ubiquitous environmental pollutant, which mainly input to the aquatic environment through discharge of industrial and agricultural waste, can be a threat to human and animal health. Selenium (Se) possesses a beneficial role in protecting animals and ameliorating the toxic effects of Cd. However, the comparative antagonistic effects of different Se sources such as inorganic, organic Se and nano-form Se on Cd toxicity are still under-investigated. Hence, the purpose of this study was to evaluate the comparative of Se sources antagonism on Cd-induced nephrotoxicity via oxidative stress and selenoproteome transcription. In the present study, Cd-diet disturbed in the system balance of 5 trace elements (Zinc (Zn), copper (Cu), Iron (Fe), Se, Cd) and impaired renal function. Se sources, including nano- Se (NS), Se- yeast (SY), sodium selenite (SS) and mixed selenium (MS) significantly recovered the balance of 4 trace elements (Zn, Cu, Cd, Se) and renal impaired indexes (blood urea nitrogen (BUN) and creatinine (CREA)). Histological appearance of Cd-treated kidney indicated renal tubular epithelial vacuoles, particle degeneration and enlarged capsular space. Ultrastructure observation results illustrated that Cd-induced mitochondrial cristae reduction, membrane disappearance, and nuclear deformation. Treatment with Se sources, NS appeared a better impact on improving kidney tissues against the pathological alterations resulting from Cd administration. Meanwhile, NS reflected a significant impact on relieving Cd-induced kidney oxidative damage, and significantly restored the antioxidant defense system of the body. Our findings also showed NS ameliorated the Cd-induced downtrends expression of selenoproteome and selenoprotein synthesis related transcription factors. Overall, NS was the most effective Se source in avoiding of Cd cumulative toxicity, improving antioxidant capacity and regulating of selenoproteome transcriptome and selenoprotein synthesis related transcription factors expression, which contributes to ameliorate Cd-induced nephrotoxicity in chickens. These results demonstrated diet supplement with NS may prove to be an effective approach for alleviating Cd toxicity and minimizing Cd -induced health risk.

Background. Recently, increasing studies have revealed that leptin is involved in the development of rheumatoid arthritis (RA). This study is aimed at exploring the association of leptin gene single nucleotide polymorphisms (SNPs) with susceptibility to RA in a Chinese population. Methods. We recruited 600 RA patients and 600 healthy controls from a Chinese population and analyzed their three leptin SNPs (rs10244329, rs2071045, and rs2167270) using the improved Multiplex Ligase Detection Reaction (iMLDR) assays. The associations of these SNPs with clinical manifestations of RA were also analyzed. Enzyme-linked immunosorbent assay (ELISA) was performed for plasma leptin determination. Results. No significant difference in either allele or genotype frequencies of these three SNPs between RA patients and healthy controls was observed (all P > 0.05 ). Association between the genotype effects of dominant, recessive models was also not found (all P > 0.05 ). No significant difference in plasma leptin levels was detected between RA patients and controls ( P > 0.05 ). Conclusion. Leptin gene (rs10244329, rs2071045, and rs2167270) polymorphisms are not associated with RA genetic susceptibility and its clinical features in the Chinese population.

Gonadal development usually involves alternative splicing of sex-related genes. Vasa, a highly conserved ATP-dependent RNA helicase present mainly in germ cells, has an important function in gonadal development. As an important sex-related gene, recent evidence indicates that different splice variants of vasa exist in many species. In this study, there was identification of two types of vasa splice variants in the Chinese mitten crab Eriocheir sinensis, termed Esvasa-l and Esvasa-s, respectively. Furthermore, splice variants of Esvasa-s were sub-divided into Esvasa-s1, Esvasa-s2, Esvasa-s3, Esvasa-s4, and Esvasa-s5, based on differing numbers of TGG repeats. Results from genomic structure analyses indicated that these forms are alternatively spliced transcripts from a single vasa gene. Results from tissue distribution assessments indicate the vasa splice variants were exclusively expressed in the gonads of male and female adult crabs. In situ hybridization results indicate Esvasa mRNA was mainly present in the cytoplasm of previtellogenic oocytes. As oocyte size increased, relative abundance of Esvasa mRNA decreased and became distributed near the cellular membrane. The Esvasa mRNA was not detectable in mature oocytes. In testis, Esvasa mRNA was detected in spermatids and spermatozoa, but not in spermatogonia and spermatocytes. Notably, results from qPCR analysis of Esvasa-l and Esvasa-s indicate there are different relative proportions during gametogenesis, implying that splice variants of the Esvasa gene may have different biological functions during crab gonadal development.

This paper analyzed the advantages and disadvantages as well as the applicability of pressurized water supply system. The following aspects were also summarized: 1) How to elect the overlying water-supply system 2) high-rise building pressurized water supply green energy saving considerations. 3) Reduction of the pipe leakage of cutting-edge technology, which focuses on the high-sensitivity, low-cost water quality monitoring technology. 4) Secondary pressurized water management, operation and scheduling as well as the establishment of relevant platforms, including wisdom water system construction, digital water supply system and secondary water supply management information system in figure.

BACKGROUND Hyperthermia (HT) has been used widely for cancer therapy, and the development of modern devices has made it more efficient. Shikonin (SHK) is a natural naphthoquinone derivative from a Chinese herb. Although the anticancer properties of SHK are evident, the underlying molecular mechanisms are not fully understood. OBJECTIVE In this study, the effects of combining low doses of SHK with mild HT were investigated in the U937 cell line. METHODS The cells were subjected to HT at 44°C for 10 min with or without SHK pretreatment, and parameters reflecting apoptosis, ROS generation and intracellular calcium elevation were evaluated by using DNA fragmentation, flow cytometry, and western blot analyses. RESULTS SHK 0.5 µM significantly enhanced HT-induced apoptosis as indicated by DNA fragmentation and caspase-3 activation with increased generation of ROS and elevation of intracellular calcium. The combined treatment also synergistically activated proapoptotic proteins and inactivated anti-apoptotic proteins. Furthermore, the phosphorylation of JNK and PKC- δ and the dephosphorylation of ERK and AKT were the upstream effects that may have compounded the induction of apoptosis. The modulatory effects of HT and SHK were abrogated with the employment of NAC and JNK-IN-8 by inactivating the MAPK pathway and cleavage of caspase-3. Intracellular calcium was also elevated and was found to be responsible for the induction of cell death evident by the DNA fragmentation with or without the employment of BAPTA-AM. CONCLUSION Conclusively, this study provides persuasive evidence that SHK in combination with HT is a propitious therapeutic way for augmentation of apoptosis and hence suggest a novel strategy for treating cancers.

Based on conserved sites and homology modeling analysis, the residue Phe581 in the Klebsiella variicola SHN-1 pullulanase was selected as the potential thermostability-related site and its role on thermostability and activity was investigated by site-saturated mutagenesis. Compared with the wild-type pullulanase, the optimum temperature of the mutants including F581L, F581Q, F581R, F581T, F581V, and F581Y was increased from 53 to 56 °C, and correspondingly the half lives of these mutants at 55 °C were increased by 4.20, 3.70, 1.90, 7.16, 3.01, and 1.75 min, respectively. By modeling the structure of the pullulanase, formation of more hydrogen bonds by single-site substitution was supposed to be responsible for the improvement of thermostability. Of these mutants, furthermore, F581L and F581V exhibited higher values of V max and k cat/K m, compared with the wild-type enzyme. Therefore, the residue Phe581 was identified as an important site relevant to the activity and thermostability of the pullulanase of K. variicola, and by mutation at this single site, the mutated enzymes with enhanced thermostability and catalytic efficiency were achieved consequently.

The reliability design method is considered based on the EMU application in our country. It is verified that the vehicle reliability obeys the exponential distribution according to the statistical testing principles and practical data statistics of some high-speed EMU, in addition, the reliability after a period of time of operation, or to say, the operational time which meet demand of the certain reliability requirement, is predicted. The reliability assessment for the EMU operating for a period of time is proceed on the basis of the suggestion table of failure rate levels obtained through quantities of statistics. Apart from this,suggestions about reliability design is proposed based on the given condition of failure rate levels. The method can be used as one of reliability assessment indicator of EMU in the future.

This paper presents a novel single-point rolling bearing fault diagnosis mechanism through vibration signal analysis. It is highlighted that the rolling bearing operational state can be well estimated by the first small set of Intrinsic Mode Function (IMF) components of the original vibration measurements through Empirical Mode Decomposition (EMD). These IMF components can be further translated into envelope spectrum by using Hilbert Transform. As a result, the difference of fault characteristic frequencies (DFCF) is derived to properly characterize different fault patterns for fault diagnosis. The suggested method is implemented and evaluated in a rolling bearing test bed for a range of failure scenarios (e.g. inner and outer raceway fault, rolling elements fault) with extensive vibration measurements. The result demonstrates that the proposed solution is effective for characterizing and detecting arrange of rolling bearing faults.quality).

The mesoporous polycarbonate-silica nanocomposite materials were synthesized through the modified sol-gel approach under acidic condition. The specific surface area, pore diameter and pore volume of polycarbonate-silica could be controlled by changing the acidity of the synthesis system. The polycarbonate-silica possess an irregular block morphology according to the scanning electron microscopy observations. With decreasing the pH value of the synthesis system, the specific surface area and pore diameter of polycarbonate-silica were raised but the pore volume was reduced. The maximum specific surface area of polycarbonate-silica was 701.71m2/g which presented by the results of Nitrogen adsorptiondesorption isotherms.

It is significant to use different techniques to improve the overall efficiency of mine water treatment, because the utilization rate of treated mine water is low and the treating cost is high. According to the characteristics of water quality of coal mine water, the influence of the magnetic field on mine water treatment was studied in this paper in order to provide a reference for mine water treatment. It was found that magnetic field has effect on surface tension and electrical conductivity of mine water. When the magnetic field strength is less than 0.3T, the surface tension of the mine water decreases with the increase of magnetic field intensity, while the magnetic field strength is more than 0.3T, the surface tension of the mine water increases with the increase of magnetic field intensity. In the range of 0.0T ~ 0.5T, the mine water conductivity increases with the increase of magnetic field intensity and, as high as 14.8 ms/cm, then along with the increase of magnetic field intensity, the conductivity remains same. The influence of magnetic field intensity, amount of coagulant and magnetization time on coagulation were studied in this paper, and their effect were also investigated by Orthogonal test. The results show that when the magnetic field intensity is 0.35 T, magnetization time is 4 min and the amount of coagulant is 3 ml/L, the coagulation effect is the best. Under the condition, the mine water turbidity fell to 0.85 NTU from 200 NTU.

Deliberate imitation which is the reproduction of another speakers voice and speech behavior can pose a threat to the security of the voice authentication system. Therefore effective characteristic parameters are the key to the anti-deliberate imitation. The study chose speech database of anti-deliberate imitation and investigated some common feature parameters separating capacity and descriptive power against voice deliberate imitation. The study compared the ranking of subjective evaluation and feature parameters Euclidean distance of imitators. The comparison results indicate that Mel frequency cepstrum coefficient (MFCC) combined with its differential cepstrum parameters (WMFCC) have the best performance for anti-deliberate imitation. And the results were validated by the experiment based on VQ speaker verification system.

Based on the platform of trolley equipment, this paper used a liquid containing gas as a sensor to establish a new testing method in order to simulate the mechanism of contre-coup injury during head decelerating impact. After pouring the liquid containing gas into the transparent skull model, the head model was sealed and fixed to the trolley. Then the trolley was trigged to impact the counterguard as the speed of 50km/h, the impacting course was recorded by a high-speed camera at the same time. Finally, the movement of the gas in the liquid during the impact was analyzed using the sequence pictures analyzing software as to ensure the area of negative pressure which appeared during the impact. The results showed that in the head decelerating impact the negative pressure can appear at the contre-coup site. This denoted that in the traffic accidents brain tissue of driver or passengers may suffer from tension injury at the contre-coup point. This paper provides a new equipment and method to research the biomechanical mechanism of contre-coup injury in traffic accidents which is helpful to understand the distribution of negative pressure in brain tissue during head decelerating impact.

Shikonin (SHK), a natural naphthoquinone derived from the Chinese medical herb Lithospermum erythrorhizon, induces both apoptosis and necroptosis in several cancer cell lines. However, the detailed molecular mechanisms involved in the initiation of cell death are still unclear. In the present study, caspase-dependent apoptosis was induced by SHK treatment at 1μM after 6h in U937 cells, with increase in DNA fragmentation, generation of intracellular reactive oxygen species (ROS), fraction of cells with low mitochondrial membrane potential (MMP), and in the expression of BH3 only proteins Noxa and tBid. Interestingly, caspase-independent cell death was also detected with SHK treatment at 10μM, observed as increase in SYTOX® Green staining and release of lactate dehydrogenase (LDH). Necrostatin-1 (Nec-1) completely inhibited the SHK-induced leakage of LDH and SYTOX® Green staining. Cell permeable exogenous glutathione (GSH) completely inhibited 1μM SHK-induced apoptosis and converted 10μM SHK-induced necroptosis to apoptosis. Gene expression profiling revealed that 353 genes were found to be significantly regulated by 1μM and 85 genes by 10μM of SHK treatment, respectively. Among these genes, the transcription factor 3 (ATF3) and DNA-damage-inducible transcript 3 (DDIT3) were highly expressed at 1μM of SHK treatment, while tumor necrosis factor (TNF) expression mainly increased at 10μM treatment. These findings provide novel information for the molecular mechanism of SHK-induced apoptosis and necroptosis.

To avoid the beginners have suffered personal injury which operate the printing machines. This paper designs a new kind of portable chain safety protection device .It is a hand-held wireless secure remote control device which can make the printing machine stopping quickly within 200 meters when accidents happen. It not only can ensure the good quality of printing products, but also eliminate or reduce the influence on the personal injury and device safety. This type of device can also be applied to safety protection of different types of printing machine and other mechanical equipment. It has a vital and practical significance.

Studies the effects of potassium ferrate combined with powdered activated carbon (PAC) to deal with the trace organics in water. It found that powder activated carbon (PAC) can adsorb the suspended solids produced by potassium ferrate removing pollutants generated in water, reduce turbidity and color result in potassium ferrate oxidation process, and remove a small amount of organics in the same time.

This paper mainly introduces the important significance on the optimization of water supply pipe network . And in terms of the present situation of and problems existing in china’s urban water suppling,puts forward a series of concrete measures for the network optimization .

This Experiment study on the simultaneous degradation effect of Cu(II) and Cr(Ⅵ) in the micro-polluted water by potassium ferrate. And the influential elements on the removal effects and probed into the degradation mechanism was analyzed. The results indicated that the removal rates of Cu(II),Cr(Ⅵ) by synergetic effect of potassium ferrate oxidation and coagulation were 76.2%、62.2%,respectively,when the optimizing conditions were as follows: oxidation pH was 7,oxidation time was 20min,flocculation pH was 9,flocculation time was 30min and dosage of potassium ferrate was 40mg/L.

Through the experiment about the potassium ferrate joint polymeric ferric sulfate removing phosphorus , add a small amount of potassium ferrate can significantly improve the phosphorus removal rate, study the optimum process conditions of the experiment about removing phosphorus, in order to make phosphorus contented in water treated reach A standard of the national urban sewage discharge standards.

Adiponectin plays an important role in the development of immune-mediated diseases. Currently published data regarding the relationship between serum/plasma levels of adiponectin and immune-mediated diseases are inconsistent. We therefore conducted this meta-analysis to explore the association of serum/plasma adiponectin levels with immune-mediated diseases in humans. Systematic literature search was conducted to identify all relevant studies. The study quality was assessed by the Newcastle-Ottawa scale. Pooled standard mean difference (SMD) with 95% confidence interval (CI) was calculated by random-effect model analysis. A total of 47 studies were included in our meta-analysis, including 27 studies of type 1 diabetes mellitus (T1DM), 9 studies of rheumatoid arthritis (RA), 7 studies of systemic lupus erythematosus (SLE), and 4 studies of ankylosing spondylitis (AS). The results revealed significant differences in serum/plasma levels of adiponectin between immune-mediated diseases and normal controls (SMD = 1.262, 95% CI 0.766-1.758, p 0.001). In the subgroup analysis stratified by disease type, the serum/plasma levels of adiponectin in T1DM, RA and SLE patients were higher than those in normal control, but not in AS patients. Moreover, in the subgroup analysis stratified by gender, in both men and women group, the serum/plasma levels of adiponectin in patients with immune-mediated diseases were higher than that in the control group. Furthermore, subgroup analyses also showed that immune-mediated diseases from Asian population, Caucasian population, mean age 40 years, and BMI ≥24 kg/m

A new method to realize 3D measurement and modeling based on Active Control System was put forward in the paper. The Active Control System included two parts: one was for the calibration of digital camera, and the other was for the six-freedom control of digital camera. This method enlarged the intersection angle and improves the intersection precision by multi-baseline photography; enlarges the angle of view by panning photography, and resolves the difficulty of matching for images with large intersection angle. The experiments proved that this method can acquire the high accuracy and improves the production efficiency.

Oil spills are almost constantly present in recent years, which cause ecological disaster of marine environment. It is important to detect the area of oil spill to take rescue measurement in time. The paper took the EnviSat Asar image as example to detect the boundary of oil spills. Firstly, the paper removed speckle noise by Histogram Equalization. Then, image segmentation was carried out by open-close mixed operation in mathematical morphology. At last, the region of oil spill was exacted independently.

To meet the demands of direct-drive petroleum equipment, developed a low-speed and high-torque permanent magnet synchronous motor. By analyzing and identifying the suitable key parameters such as permanent magnet structure parameters, air gap length, type and size of Stator Slot and so on, designed permanent magnet synchronous motor with the torque 10800 Nm, the rated speed 30 r/min and high efficiency and power factor. Obtained the flux density distribution situation and characteristic curves under no-load working condition through simulation and analysis. Manufactured the low-speed and high-torque permanent magnet synchronous motor and applied it to pumping unit. Test results show that this motor has many advantages such as wide range of speed regulation, small torque ripple, smooth operation and other characteristics. The Direct-drive Pumping Unit used this permanent magnet synchronous motor can save 20% energy than the beam pumping unit, so its social and economic benefits are significant.

In order to obtain good static and dynamic characteristics of the 2-DOF micro-positional stage, improved design of developed stage were done. First, the part of displacement coupling for the stage was improved, which making the stage only have linear displacement at driving direction; Second, in order to acquire the highest natural frequency under the condition of given output displacement, micro-positional stage was designed by using the size optimization method. Finally, the static and dynamic characteristics of the stage were analyzed by the finite element software ANSYS. The finite element results show that: the stage completely eliminates the displacement coupling after improving design; under the condition of given 30 μm output displacement, natural frequency reaches 1.86 kHz.

Based on the requirements of that the finger can move in parallel, and the displacement of the finger can be detected, the micro-gripper driven by piezoelectric actuator is designed based on the displacement amplification structure with the flexure hinge. The static analysis, the modal analysis, the harmonic response analysis and the transient response analysis of the micro-gripper are carried out by using the finite element analysis software ANSYS. The results of the finite element analysis show that the finger is fully able to move in parallel, and can detect the displacement of the finger; the maximum displacement of the finger is about 101 μm, the first natural frequency is about 130 Hz; the finger tip displacement under the 1 μm step input is about 20 μm, the fingertip vibration is about ±2 μm.

Satellite avionics was introduced to gain mass reduction, lower cost, modular and standard equipment. Fault detection, isolation and recovery is one of the main way to upgrade the autonomy of satellites. Built-in-test is one of the most prevalent and effective fault detection method, according to the structure and characteristics of satellite avionics, the BIT of satellite avionics was designed and introduced.

Three forms of animals’ adhesion were analyzed: hairy adhesive pads, smooth adhesive pads and claw. Scanning electron microscope (SEM) was used to observe the surface of friction plate with different particle diameters. It’s found that the particle could be seen as conical shape. The self-designed lever-like testing equipment was also used to measure the adhesive force of drosophila melanogaster contacting 6 substrates with different roughness. It was shown that adhesive force decreased first and finally rose with the increase of surface roughness. When the roughness (R value) was about 68.5nm, it showed that the lowest adhesive force was 0.0085mN. Then model of the Van der Waals force was established. In this model, contact form between seta and the substrate was divided into five states, and the theoretical adhesive force at each state was calculated, which successfully simulated with the actual value. However, when surface roughness reached the situation that the gap between adjacent particles was greater than 2μm, the gripping function of the claw made the actual value greater than theoretical one. It was concluded that adhesive force was a compound function of the Van der Waals force generated by hairy adhesive force and the gripping force generated by claw. So it was also speculated that the fibrous fine pads of insects were the basic form adhesion on smooth surface of the substrate.

The configuration of a new swirlmeter is designed. The hollow cylindric fluid axial-symmetrically in cylindric pipe is analyzed, and then the algebra expression of meter coefficient is deduced. An impeller of swirl generator attaching to a specimen of swirlmeter is designed according to the deducing process. Simulations and experiments test the method of measuring flowrate by measuring the rotation frequency of ball is feasible. Comparing the results of simulations and experiments with that of algebra expression derived from deducing in theory the maximal error is 7.8%. At last, the reasons resulting in the errors are discussed which the next researches will continue to minus the errors. The conclusion will be applied in designing the impeller of swirl generator and measuring the flow by the new swirlmeter.

In order to obtain higher accuracy and faster calculation speed of hysteresis model of the piezoelectric micro-positional stage, the traditional PI hysteresis model is improved in this paper. First, based on the fact of that the slopes of the first half of the initial loading curve for the piezoelectric micro-positional stage change much while the slopes of the second half change little, the PI hysteresis model of the stage is established by the non-equiinterval threshold value. Then, the established model is identified by optimizing the minimum L22-norm of the model errors. The results show that the maximum error and average error of the model are 0.71 μm and 0.23 μm in the displacement range of 22.75 μm, respectively.

Considering assembly and disassembly of micro-gripper, connection and isolation of lead, a new micro-gripper based on piezoelectric bimorphs is designed by using finite element analysis. First, a detailed structure of the micro-gripper is designed based on piezoelectric bimorph cantilevered beams. Second, static and dynamic characteristics of the micro-gripper are analyzed by piezoelectric coupling field analysis technique of ANSYS. For the static performance, there is a linear relationship between displacement and driving voltage of the micro-gripper. The maximum displacement of the micro-gripper finger is about 81.9 μm under the maximum driving voltage 200 V. For dynamic performance, the natural frequency and the response time of the gripper is about 45 Hz and 1.1 ms, respectively. The micro-gripper could meet the designed needs.

The paper presents a motor bearing fault diagnosis method based on MSICA (Multi-scale Independent Principal Component Analysis) and LSSVM (Least Squares Support Vector Machine). MSICA is introduced into motor fault diagnosis. First, wavelet decomposition is used, and then ICA models are built by wavelet coefficients in each scale, which detect fault, and finally LSSVM is used to classify fault type. Conclusions are obtained from the analysis of the experimental data provided by Case Western Reserve University’s Bearing Data Website. And it indicates that the proposed method is simple and effective.