28 results on '"Xinying Xue"'
Search Results
2. Does propofol definitely improve postoperative cognitive dysfunction?—a review of propofol-related cognitive impairment
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Pengfei, Liu, Sheng, Zhao, Hui, Qiao, Tianzuo, Li, Weidong, Mi, Zhipeng, Xu, and Xinying, Xue
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Postoperative Complications ,Postoperative Cognitive Complications ,Anesthetics, Inhalation ,Biophysics ,Humans ,Cognitive Dysfunction ,General Medicine ,Propofol ,Biochemistry ,Anesthetics, Intravenous - Abstract
Postoperative cognitive dysfunction (POCD) is a common brain function-related complication after surgery. In addition to old age being an independent risk factor, anesthetics are also important predisposing factors. Among them, propofol is the most commonly used intravenous anesthetic in clinical practice. It has a rapid onset, short half-life, and high recovery quality. Many studies report that propofol can attenuate surgery-induced cognitive impairment, however, some other studies reveal that propofol also induces cognitive dysfunction. Therefore, this review summarizes the effects of propofol on the cognition, and discusses possible related mechanisms, which aims to provide some evidence for the follow-up studies. more...
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- 2022
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3. Gene, virulence and related regulatory mechanisms in Cryptococcus gattii
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Yemei Huang, Xuelei Zang, Chen Yang, Hengyu Deng, Xidong Ma, Mei Xie, Meng Zhou, Jialin Song, and Xinying Xue
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Biophysics ,General Medicine ,Biochemistry - Published
- 2022
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4. Spin regulation on (Co,Ni)Se2/C@FeOOH hollow nanocage accelerates water oxidation
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Yu Gu, Xiaolei Wang, Muhammad Humayun, Linfeng Li, Huachuan Sun, Xuefei Xu, Xinying Xue, Aziz Habibi-Yangjeh, Kristiaan Temst, and Chundong Wang
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General Medicine - Published
- 2022
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5. Atorvastatin attenuates surgery-induced BBB disruption and cognitive impairment partly by suppressing NF-κB pathway and NLRP3 inflammasome activation in aged mice
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Pengfei Liu, Lei Guan, Tianzuo Li, Yanting Hu, Hui Qiao, Quansheng Gao, Teng Gao, Weixuan Sheng, Xinying Xue, and Jingwen Jiang
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Aging ,medicine.medical_specialty ,Inflammasomes ,Atorvastatin ,Biophysics ,Morris water navigation task ,Blood–brain barrier ,Occludin ,Biochemistry ,Neuroprotection ,Mice ,03 medical and health sciences ,0302 clinical medicine ,NLR Family, Pyrin Domain-Containing 3 Protein ,Animals ,Medicine ,Cognitive Dysfunction ,Neuroinflammation ,030304 developmental biology ,0303 health sciences ,business.industry ,NF-kappa B ,Inflammasome ,General Medicine ,Surgery ,medicine.anatomical_structure ,Blood-Brain Barrier ,Apoptosis ,Surgical Procedures, Operative ,business ,030217 neurology & neurosurgery ,Signal Transduction ,medicine.drug - Abstract
In clinic, perioperative neurocognitive disorder is becoming a common complication of surgery in old patients. Neuroinflammation and blood-brain barrier (BBB) disruption are important contributors for cognitive impairment. Atorvastatin, as a strong HMG-CoA reductase inhibitor, has been widely used in clinic. However, it remains unclear whether atorvastatin could prevent anesthesia and surgery-induced BBB disruption and cognitive injury by its anti-inflammatory property. In this study, aged C57BL/6J mice were used to address this question. Initially, the mice were subject to atorvastatin treatment for 7 days (10 mg/kg). After a simple laparotomy under 1.5% isoflurane anesthesia, Morris water maze was performed to assess spatial learning and memory. Western blot analysis, immunohistochemistry, and enzyme-linked immunosorbent assay were used to examine the inflammatory response, BBB integrity, and cell apoptosis. Terminal-deoxynucleotidyl transferase mediated nick end labeling assay was used to assess cell apoptosis. The fluorescein sodium and transmission electron microscopy were used to detect the permeability and structure of BBB. The results showed that anesthesia and surgery significantly injured hippocampal-dependent learning and memory, which was ameliorated by atorvastatin. Atorvastatin could also reverse the surgery-induced increase of systemic and hippocampal cytokines, including IL-1β, TNF-α, and IL-6, accompanied by inhibiting the nuclear factor kappa-B (NF-κB) pathway and Nucleotide-Binding Oligomerization Domain, or Leucine Rich Repeat and Pyrin Domain Containing 3 (NLRP3) inflammasome activation, as well as hippocampal neuronal apoptosis. In addition, surgery triggered an increase of BBB permeability, paralleled by a decrease of the ZO-1, occludin, and Claudin 5 proteins in the hippocampus. However, atorvastatin treatment could protect the BBB integrity from the impact of surgery, by up-regulating the expressions of ZO-1, occludin, and Claudin 5. These findings suggest that atorvastatin exhibits neuroprotective effects on cognition in aged mice undergoing surgery. more...
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- 2021
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6. Significance of differential expression profiles of ABC transporters in azole susceptibility between Cryptococcus gattii VGI and VGII strains
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Xinying Xue, Xuelei Zang, Meng Xiao, Lifeng Wang, Hua Wu, Xidong Ma, Ningxin Wu, Hengyu Deng, Meng Zhou, Lei Pan, Dingxia Shen, and Jianxin Wang
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Azoles ,Antifungal Agents ,Infectious Diseases ,Cryptococcus neoformans ,Animals ,ATP-Binding Cassette Transporters ,Cryptococcus gattii ,Cryptococcosis ,Microbial Sensitivity Tests ,General Medicine ,Multilocus Sequence Typing - Abstract
Azoles were used as the primary antifungal agents to treat the Cryptococcus gattii infection. Evidence showed that subtypes of C. gattii respond differently to azoles, but the mechanism is largely elusive. In this study, we aimed to find the mechanisms of differences in azole drug susceptibility in different subtypes of C. gattii. Eight clinical strains of C. gattii were collected for molecular typing, multilocus sequence typing (MLST) analysis, and antifungal susceptibility testing. Based on drug susceptibility differences, the RNA sequencing data were analyzed to find candidate azole drug susceptibility genes, and qPCR validation was performed. Five VGI subtypes and three VGII subtypes were identified among the eight strains of C. gattii. The clinical isolates showed high genetic diversity, and seven sequence types (STs) were identified. The geometric mean (GM) of minimum inhibitory concentration (MIC) for fluconazole, voriconazole, and itraconazole of VGI subtype was significantly lower than that of VGII subtype, and genes related to transporter activities were differentially expressed between VGI and VGII strains. The results of the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the DEGs (differential expressed genes) were found to be enriched in multiple ABC transporters. We further performed qPCR to quantify the expression level of seven ABC transporters. We found that ABC transporters ATM1, MDR1, PDR5, PDR5-3, and PXA2 were expressed significantly higher in VGII strains than in VGI strains. Our work revealed four novel ABC transporters, ATM1, PDR5, PDR5-3, and PXA2, promising candidate targets regulating azole susceptibility in C. gattii strains. Lay Summary Azoles were used as the primary antifungal agents for treating Cryptococuss gattii infection. Since subtypes of C. gattii respond differently to azoles. We analyzed mRNA expression profiles of different subtypes and identified four ABC transporters that could be potential genes regulating azole sensitivity. more...
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- 2022
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7. Reverse engineering a predictive signature characterized by proliferation, DNA damage, and immune escape from stage I lung adenocarcinoma recurrence
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Yang Ge, Manyu Li, Jun Peng, Nathaniel Weygant, Liyang Zhang, Tianbo Gao, Parthasarathy Chandrakesan, C. Benedikt Westphalen, Kenneth J. Vega, Xinying Xue, Jiannan Yao, Dongfeng Qu, and Guangyu An
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Male ,Oncology ,medicine.medical_specialty ,Lung Neoplasms ,FOLFIRINOX ,Biophysics ,Adenocarcinoma of Lung ,Biochemistry ,Disease-Free Survival ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Antineoplastic Combined Chemotherapy Protocols ,medicine ,Humans ,Gene Regulatory Networks ,Stage (cooking) ,Aged ,Neoplasm Staging ,030304 developmental biology ,Cisplatin ,0303 health sciences ,Proportional hazards model ,business.industry ,Cancer ,General Medicine ,Middle Aged ,medicine.disease ,Gemcitabine ,Survival Rate ,030220 oncology & carcinogenesis ,Biomarker (medicine) ,Adenocarcinoma ,Female ,Tumor Escape ,Neoplasm Recurrence, Local ,business ,DNA Damage ,medicine.drug - Abstract
Identifying early-stage cancer patients at risk for progression is a major goal of biomarker research. This report describes a novel 19-gene signature (19-GCS) that predicts stage I lung adenocarcinoma (LAC) recurrence and response to therapy and performs comparably in pancreatic adenocarcinoma (PAC), which shares LAC molecular traits. Kaplan-Meier, Cox regression, and cross-validation analyses were used to build the signature from training, test, and validation sets comprising 831 stage I LAC transcriptomes from multiple independent data sets. A statistical analysis was performed using the R language. Pathway and gene set enrichment were used to identify underlying mechanisms. 19-GCS strongly predicts overall survival and recurrence-free survival in stage I LAC (P=0.002 and P more...
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- 2020
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8. MIR205HG facilitates carcinogenesis of lung squamous cell carcinoma in vitro revealed by long noncoding RNA profiling
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Xinying Xue, Liang'an Chen, Yunjing Li, Zhen Wu, Fei Xu, Yongfu Ma, Yan Chang, Yu Dai, Wei Zhao, Chunsun Li, and Yang Liu
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0301 basic medicine ,Cell growth ,Biophysics ,Cell migration ,General Medicine ,Biology ,medicine.disease ,medicine.disease_cause ,Biochemistry ,Long non-coding RNA ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Downregulation and upregulation ,Cell culture ,Apoptosis ,030220 oncology & carcinogenesis ,medicine ,Cancer research ,Lung cancer ,Carcinogenesis - Abstract
As a subtype of non-small-cell lung cancer, lung squamous cell carcinoma (LUSC) accounts for one-fifth of all lung cancers. Unfortunately, no specific targetable aberration has yet been identified. Hence, it is of huge urgency and potential to identify aberrantly regulated genes in LUSC. Here, five pairs of LUSC samples and their corresponding adjacent tissues were subject to whole transcriptome sequencing. Our results showed that CTD-2562J17.6 and FENDRR were significantly downregulated while MIR205HG, LNC_000378, RP11-116G8.5, RP3-523K23.2, and RP5-968D22.1 were significantly upregulated in all five LUSC samples. Importantly, MIR205HG was upregulated in LUSC clinical samples as well as in LUSC cell lines. Interestingly, our results demonstrated that the expression level of MIR205HG is positively correlated with the malignancy. In addition, MIR205HG is required for LUSC cell growth and cell migration. Most importantly, our results showed that MIR205HG prohibits LUSC apoptosis via regulating Bcl-2 and Bax. Taken together, our data shed lights on the lncRNA regulatory nexus that controls the carcinogenesis of LUSC and provided potential novel diagnostic markers and therapeutic targets for LUSC. more...
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- 2020
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9. Characteristics of the Computed Tomography Imaging Findings in 72 Patients with Airway-Invasive Pulmonary Aspergillosis
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Junping Pan, Ligong Chang, Yu-Chen Chen, Jing Wu, Qian Zhang, Xinying Xue, Xin Lin, and Tao Zhang
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Adult ,Male ,medicine.medical_specialty ,China ,Computed tomography ,Immunocompromised Host ,Young Adult ,Main Bronchus ,Clinical Research ,Multidetector Computed Tomography ,medicine ,Humans ,Aged ,Retrospective Studies ,Aged, 80 and over ,Invasive Pulmonary Aspergillosis ,Bronchus ,Bronchiectasis ,Lung ,medicine.diagnostic_test ,business.industry ,Retrospective cohort study ,General Medicine ,Invasive pulmonary aspergillosis ,respiratory system ,Middle Aged ,medicine.disease ,Prognosis ,Classification ,respiratory tract diseases ,medicine.anatomical_structure ,Female ,Radiology ,business ,Airway ,Tomography, X-Ray Computed ,Follow-Up Studies - Abstract
BACKGROUND This multiple-center retrospective study aimed to investigate computed tomography (CT) imaging findings in 72 patients with airway-invasive pulmonary aspergillosis. MATERIAL AND METHODS Seventy-two patients with airway-invasive pulmonary aspergillosis confirmed by pathology results were divided into 3 types according to image characteristics. Type I involved the trachea or the main bronchus. Type II involved the lobular and segmental bronchi, which manifested early as bronchial wall thickening, and later development was divided into types IIa and IIb. Type IIa manifested as bronchiectasis, and type IIb manifested as consolidation around the bronchus. Type III involved the bronchioles and pulmonary parenchyma, with tree-in-bud sign and acinar nodules around. CT signs of the various types and their differentiation were investigated. RESULTS The main clinical manifestations of the 72 patients with airway-invasive pulmonary aspergillosis were shortness of breath (55/72, 76.4%), cough (40/72, 55.6%), expectoration (35/72, 48.6%), dyspnea (8/72, 11.1%), weight loss (2/72, 2.8%), and fever (30/72, 41.7%). CT typing identified 3 types: 2 patients (2.8%) had type I, presenting as thickening of trachea or main bronchial walls; 3 patients (4.2%) had early type II, manifesting as thickening of lobular or segmental bronchial walls; 27 patients (37.5%) developed type IIa, manifesting as bronchiectasis; 22 patients (30.6%) had type IIb, manifesting as consolidation around the bronchus; and 18 patients (25.0%) had type III, presenting as nodules and patchy shadows with small cavities in the periphery of the lung. CONCLUSIONS Airway pulmonary aspergillosis has characteristic imaging findings, which can help early clinical diagnosis through classification according to CT imaging characteristics. more...
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- 2021
10. Comparison of clinical features of pulmonary cryptococcosis with and without central nervous system involvement in China
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Xuelei Zang, Chongchong Wu, Jie Gao, Tianjiao Jiang, Lei Pan, Lifeng Wang, Dingxia Shen, Dongliang Lin, Xidong Ma, Hui Deng, and Xinying Xue
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0301 basic medicine ,Medicine (General) ,Pathology ,medicine.medical_specialty ,030106 microbiology ,Central nervous system ,Cryptococcus ,cavity ,Biochemistry ,030218 nuclear medicine & medical imaging ,03 medical and health sciences ,R5-920 ,0302 clinical medicine ,medicine ,Pulmonary cryptococcosis ,risk factors ,In patient ,fever ,biology ,business.industry ,Biochemistry (medical) ,Cell Biology ,General Medicine ,central nervous system ,biology.organism_classification ,medicine.anatomical_structure ,business ,Retrospective Clinical Research Report - Abstract
Objective This study aimed to compare the clinical features of pulmonary cryptococcosis (PC) in patients with and without central nervous system (CNS) involvement. Methods We retrospectively reviewed demographics, presenting symptoms, radiographic features, and laboratory findings of patients diagnosed with PC in 28 hospitals from 2010 to 2019. Risk factors for CNS involvement were analyzed using logistic regression models. Result A total of 440 patients were included, and 36 (8.2%) had CNS involvement. Significant differences in fever, headache, and chills occurred between the two groups (overall and with/without CNS involvement) for fever (17.8% [78/440]; 52.8% vs. 14.6% of patients, respectively), headache (4.5% [20/440]; 55.6% vs. 0% of patients, respectively), and chills (4.3% [19/440]; 13.9% vs. 3.5% of patients, respectively). The common imaging manifestation was nodules (66.4%). Multivariate analysis showed that cavitation (adjusted odds ratio [AOR] = 3.552), fever (AOR = 4.182), and headache were risk factors for CNS involvement. Routine blood tests showed no differences between the groups, whereas in cerebrospinal fluid the white blood cell count increased significantly and glucose decreased significantly. Conclusion In patients with PC, the risk of CNS involvement increases in patients with headache, fever, and cavitation; these unique clinical features may be helpful in the diagnosis. more...
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- 2021
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11. Pulmonary mucosa-associated lymphoid tissue lymphoma: CT findings and pathological basis
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Pei Nie, Xuelei Zang, Xidong Ma, Shao-hong Zhao, Shuo Zhao, Chongchong Wu, Jie Gao, Xinying Xue, Wanli Bi, Shifeng Yang, Yan Deng, Hui Deng, Xinxin Yu, Jun Han, Ximing Wang, and Idorenyin Polycarp Asuquo more...
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Male ,medicine.medical_specialty ,Lung Neoplasms ,Mucosa-Associated Lymphoid Tissue Lymphoma ,Primary pulmonary lymphoma ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Humans ,Halo sign ,Pathological ,Retrospective Studies ,Lung ,business.industry ,General Medicine ,Lymphoma, B-Cell, Marginal Zone ,Middle Aged ,Marginal zone ,medicine.disease ,Prognosis ,Lymphoma ,Lymphatic system ,medicine.anatomical_structure ,Oncology ,030220 oncology & carcinogenesis ,030211 gastroenterology & hepatology ,Surgery ,Female ,Radiology ,medicine.symptom ,business ,Tomography, X-Ray Computed ,Follow-Up Studies - Abstract
Background Pulmonary mucosa-associated lymphoid tissue lymphoma (MALToma) is the most frequent subset of primary pulmonary lymphoma. This study aimed to identify radiologic characteristics of pulmonary MALToma based on computed tomography (CT) observations and pathologic features, and further investigate its prognosis. Methods Sixty-six patients (55.4 ± 10.9 years; 51.5% male) diagnosed as pulmonary MALToma by pathology were retrospectively enrolled. According to distributions and features of lesions shown on CT, patients were divided into three patterns, including single nodular/mass, multiple nodular/mass, and pneumonia-like consolidative. Results Variety of the location and extent of the lymphomatous infiltration accounted for different characteristics demonstrated at CT. The pneumonia-like consolidative pattern was the most frequent pattern observed in 42 patients (63.6%), followed by single nodular/mass (21.2%) and multiple nodular/mass (15.2%). CT features included air bronchogram (72.7%), well-marginated halo sign (53.0%), coarse spiculate with different lengths (72.7%), angiogram sign (77.1% of 35 patients), peribronchovascular thickening (48.5%), irregular cavitation (16.7%) and pulmonary cyst (7.6%). The estimated 5-year cumulative overall survival rate of pulmonary MALToma was 100.0%. Conclusions Pulmonary MALToma demonstrates several characteristics at CT. Identification of the significant pulmonary abnormalities of this indolent disease entity might be helpful for early diagnosis and optimal treatment. more...
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- 2020
12. Cryptococcosis caused by cryptococcus gattii: 2 case reports and literature review
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Idorenyin Polycarp Asuquo, Chong Qin, Mingming Meng, Xinying Xue, Chongchong Wu, Longting Zhao, Xuelei Zang, Lei Pan, Yao Meng, Xidong Ma, Hui Deng, and Jie Gao
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Adult ,Male ,Antifungal Agents ,Adolescent ,Genotype ,Treatment outcome ,Observational Study ,epidemiologically ,Flucytosine ,Spinal Puncture ,Treatment Refusal ,03 medical and health sciences ,Immunocompromised Host ,Young Adult ,0302 clinical medicine ,Asian People ,Central Nervous System Fungal Infections ,Recurrence ,Amphotericin B ,parasitic diseases ,medicine ,Prevalence ,Humans ,infections ,030212 general & internal medicine ,Cryptococcus gattii ,Aged ,immunocompetence ,biology ,Geography ,business.industry ,General Medicine ,Cryptococcosis ,Middle Aged ,bacterial infections and mycoses ,medicine.disease ,biology.organism_classification ,Treatment Outcome ,030220 oncology & carcinogenesis ,Immunology ,Administration, Intravenous ,Drug Therapy, Combination ,Female ,business ,Research Article - Abstract
Cryptococcosis caused by Cryptococcus gattii, is a life threatening fungal infection with recently increasing prevalence. C. gattii is a species complex comprising multiple independent species. However, many biological characteristics and clinical features of cryptococcosis due to C. gattii are relatively less well defined. In this paper, we identify two cases of C. gattii infection, and laboratory findings of genotype VGI and VGII in two groups of apparently immunocompetent Chinese individuals respectively. Upon detailed review of all 35 cases of C. gattii infections, it was observed that C. gattii can cause debilitating illness in both immunocompetent and immunocompromised individuals. Cryptococcosis due to C. gattii is a serious systemic fungal infection, with pulmonary central nervous system tropism. Epidemiologically, C. gattii infection is not only restricted in tropical and subtropical regions, but also in other geographical settings. more...
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- 2020
13. Comparison of radiologic characteristics and pathological presentations of primary pulmonary lymphoma in 22 patients
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Ningxin Wu, Lei Pan, Yanchao Wang, Xuan Wang, Jun Han, Fantao Zhang, Chongchong Wu, Rongjian Lu, Dahai Zhao, Jie Gao, Zhaoyu Wang, and Xinying Xue
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radiologic characteristics ,Pathology ,medicine.medical_specialty ,Medicine (General) ,Lung Neoplasms ,pathological presentations ,Computed tomography ,Primary pulmonary lymphoma ,Biochemistry ,030218 nuclear medicine & medical imaging ,03 medical and health sciences ,0302 clinical medicine ,R5-920 ,hemic and lymphatic diseases ,Positron Emission Tomography Computed Tomography ,Medicine ,Humans ,B-cell lymphoma ,Pathological ,Retrospective Studies ,medicine.diagnostic_test ,business.industry ,Mechanism (biology) ,Biochemistry (medical) ,computed tomography ,Cell Biology ,General Medicine ,Lymphoma, B-Cell, Marginal Zone ,medicine.disease ,mucosa-associated lymphoid tissue ,030220 oncology & carcinogenesis ,business ,Tomography, X-Ray Computed ,Mucosa-associated lymphoid tissue ,Retrospective Clinical Research Report - Abstract
Objective This study was performed to compare the radiologic characteristics and pathological presentations of primary pulmonary lymphoma (PPL), explore the possible mechanism underlying its development, summarize its radiologic characteristics, and improve the accuracy of its diagnosis. Methods The medical records of 22 patients pathologically diagnosed with PPL were retrospectively analyzed. Results Chest computed tomography (CT) demonstrated single or multiple nodules and masses in the lungs, patchy opacities or consolidation along the bronchovascular bundle, and no significantly enlarged mediastinal or hilar lymph nodes. All 22 cases of PPL were classified as non-Hodgkin’s lymphoma (NHL) by transbronchial biopsy, CT-guided needle biopsy, and postoperative pathology. Most (16 cases) were marginal-zone B-cell lymphomas of mucosa-associated lymphoid tissue (MALT). Twelve patients had air bronchograms within the lesion, and 13 showed ill-defined lesions with ground-glass brush-like changes. Conclusion PPL is a rare lung tumor, and most are classified as MALT lymphoma, a subtype of NHL. Chest CT can help to diagnose this disease. Positron emission tomography (PET)/CT is of great clinical value for evaluation of the lesion and patient’s general condition. The possibility of PPL should be considered in patients with characteristic CT and PET/CT findings and mild clinical symptoms, and early treatment should be administered. more...
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- 2019
14. Exosomal miRNA profiling before and after surgery revealed potential diagnostic and prognostic markers for lung adenocarcinoma
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Xidong Ma, Chong Qin, Jun Han, Chen Wang, Idorenyin Polycarp Asuquo, Zhiqiang Xue, Hui Deng, Rui Guo, Xuelei Zang, Jianxin Wang, Haijiao Wang, Gao Quansheng, Jiaxin Wen, Sanhong Liu, and Xinying Xue
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Adult ,Male ,medicine.medical_specialty ,Lung Neoplasms ,Biophysics ,Adenocarcinoma of Lung ,Kaplan-Meier Estimate ,Adenocarcinoma ,Exosomes ,Biochemistry ,Exosome ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,Carcinoma, Non-Small-Cell Lung ,microRNA ,medicine ,Biomarkers, Tumor ,Humans ,Survival analysis ,030304 developmental biology ,Aged ,0303 health sciences ,Lung ,Exosome Multienzyme Ribonuclease Complex ,business.industry ,Gene Expression Profiling ,General Medicine ,Middle Aged ,medicine.disease ,Prognosis ,Microvesicles ,Surgery ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,medicine.anatomical_structure ,ROC Curve ,030220 oncology & carcinogenesis ,Cancer cell ,Female ,business - Abstract
Exosome is a crucial manner for cancer cell to cell communication and circulating exosomes sever as promising diagnostic and prognostic markers for various types of diseases. A predominant type of cargo of exosome is small RNAs, especially miRNAs. Here, we profiled plasma exosomal miRNAs of six lung adenocarcinoma patients before and after surgery, as well as six healthy individuals as normal control. Our profiling revealed 38 upregulated and 37 downregulated exosomal miRNAs in the plasma of lung adenocarcinoma patients. Additionally, we found that most upregulated miRNAs were increased in the lung adenocarcinoma samples of TCGA database. We further evaluated the correlation between the upregulated exosomal miRNAs and overall survival with Kaplan-Meier survival analysis using online databases. Our results suggested that exosomal miR-151a-5p, miR-10b-5p, miR-192-5p, miR-106b-3p, and miR-484 are potential prognostic markers for lung adenocarcinoma. Importantly, we validated candidate miRNAs in lung adenocarcinoma patients before and after surgery as well as in healthy controls and found that miR-484 was significantly increased in the plasma of lung adenocarcinoma patients and strikingly decreased post-surgery. Hence, we provided novel information on lung adenocarcinoma-derived exosomal miRNA and potential non-invasive diagnostic and prognostic markers for lung adenocarcinoma. more...
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- 2019
15. Exosomal piRNA profiling revealed unique circulating piRNA signatures of cholangiocarcinoma and gallbladder carcinoma
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Rong Liu, Xinjin Gu, Sanhong Liu, Chong Qing, Hui Deng, Chen Wang, and Xinying Xue
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Adult ,Male ,Small RNA ,information science ,Biophysics ,Piwi-interacting RNA ,Exosomes ,Biochemistry ,Extracellular vesicles ,Exosome ,Cholangiocarcinoma ,03 medical and health sciences ,0302 clinical medicine ,parasitic diseases ,Carcinoma ,medicine ,Biomarkers, Tumor ,Humans ,cardiovascular diseases ,RNA, Neoplasm ,030304 developmental biology ,Aged ,Aged, 80 and over ,0303 health sciences ,business.industry ,Gallbladder ,fungi ,General Medicine ,Middle Aged ,medicine.disease ,Microvesicles ,medicine.anatomical_structure ,Bile Duct Neoplasms ,Biliary tract ,030220 oncology & carcinogenesis ,cardiovascular system ,Cancer research ,Female ,Gallbladder Neoplasms ,business ,Cell-Free Nucleic Acids - Abstract
Cholangiocarcinoma (CCA) and gallbladder carcinoma (GBC) are biliary tract cancers with poor five-year survival and high recurrence rates. Both CCA and GBC patients suffer from lack of circulating diagnostic biomarkers at the early stage. Extracellular vesicles, especially exosomes, have been emerged as promising diagnostic sources for cancers due to easy and quick accessibility. Hence, identification of exosomal biomarkers provides a novel strategy for CCA and GBC diagnosis. Here, five CCA patients and four GBC patients were enrolled for exosomal small RNA sequencing. Our data showed that exosomal piwi-interacting RNA (piRNA) populations were altered in the plasma of CCA and GBC patients. In comparison to healthy individuals, 694 and 323 piRNAs were upregulated in CCA and GBC, respectively, while 36 and 191 piRNAs were downregulated. Interestingly, sequencing results predicted that piR-2660989, piR-10506469, piR-20548188, piR-10822895, piR-hsa-23209, and piR-18044111 were upregulated in both CCA and GBC plasma. Importantly, we further included blood samples from 50 health individuals, 40 CCA patients, and 25 GBC patients and found that piR-10506469 were significantly increased in the exosomes of plasma from both CCA and GBC patients. Moreover, we analyzed the expression levels of differentially expressed exosomal piRNAs in the plasma of CCA and GBC patient before and after surgeries and found that piR-10506469 and piR-20548188 were significantly decreased in patients underwent surgeries. Taken together, our data revealed that exosomal piRNAs those are differentially expressed in CCA and GBC plasma may serve as potential biomarkers for the diagnosis of CCA and GBC. more...
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- 2019
16. Bone marrow stromal cells induced activation of nuclear factor κB signaling protects non-Hodgkin’s B lymphoma cells from apoptosis
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Xiaorong Ma, Mingming Meng, Zuojun Liu, Li-Xin Sun, Tuo Su, Wei Han, Sheng Zhao, Jiakai Li, Xinmin Ding, Hong Jiang, Yali Xu, Jin Qian, Xiao-Bin Li, Xinying Xue, and Lei Pan
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0301 basic medicine ,Pathology ,medicine.medical_specialty ,Lymphoma, B-Cell ,Stromal cell ,CD30 ,Primary Cell Culture ,Apoptosis ,03 medical and health sciences ,hemic and lymphatic diseases ,B-Cell Activating Factor ,Tumor Cells, Cultured ,Tumor Microenvironment ,medicine ,Humans ,RNA, Small Interfering ,Cells, Cultured ,B cell ,Tumor microenvironment ,Chemistry ,RANK Ligand ,Mesenchymal stem cell ,NF-kappa B ,Mesenchymal Stem Cells ,General Medicine ,Coculture Techniques ,Neoplasm Proteins ,Gene Expression Regulation, Neoplastic ,030104 developmental biology ,medicine.anatomical_structure ,Cell culture ,Cancer research ,RNA Interference ,Bone marrow ,Signal Transduction - Abstract
The microenvironment encompassing a variety of non-malignant cells in close proximity with malignant tumor cells has been well known to significantly affect the behavior of tumor cells. In this study, we therefore studied the mechanism of bone marrow stromal cells in protection of lymphoma cells from spontaneous apoptosis. We demonstrated that adhesion of the freshly isolated lymphoma B cells to bone marrow stromal cells or freshly isolated lymphoma stromal cells inhibited B cell spontaneous apoptosis in culture. This inhibition of apoptosis correlated with decreased cleavage of caspase-3/8 and increased activation of canonical and non-canonical NF-κB signaling pathway. In addition to BAFF signaling which has been reported as a functional determinant for B lymphoma cell survival in the bone marrow environment, we demonstrated RANKL from BMSCs works synergistically with BAFF to activate NF-κB signaling pathway and thus protects lymphoma B cells from spontaneous apoptosis. more...
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- 2016
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17. Comparative study of primary pulmonary cryptococcosis with multiple nodules or masses by CT and pathology
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Limin Feng, Bo Wei, Yuguang Wang, Jie Gao, Shao-hong Zhao, Dongxu Wang, Chongchong Wu, Xidong Ma, and Xinying Xue
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0301 basic medicine ,Cancer Research ,Pathology ,medicine.medical_specialty ,Tuberculosis ,030106 microbiology ,H&E stain ,Disease ,multi-slice spiral computed tomography ,03 medical and health sciences ,0302 clinical medicine ,Immunology and Microbiology (miscellaneous) ,medicine ,Lung cancer ,Pathological ,Cryptococcus neoformans ,biology ,business.industry ,Cancer ,Articles ,pulmonary cryptococcosis ,General Medicine ,biology.organism_classification ,medicine.disease ,Staining ,lung cancer ,tuberculosis ,030220 oncology & carcinogenesis ,pathological ,business - Abstract
The manifestations of pulmonary cryptococcosis with multiple nodules or masses on computed tomography (CT) are diverse and difficult to differentiate from those of lung cancer and pulmonary tuberculosis. The present study compared the multislice spiral CT signs with pathological results and used the pathological results to explain the CT signs with the aim of improving the accuracy of the diagnosis of this disease. A retrospective analysis of 20 patients with primary pulmonary cryptococcosis with multiple nodules or masses was performed. Based on the CT signs, eight patients had been misdiagnosed with lung cancer accompanied by intrapulmonary metastasis andthree patients had been misdiagnosed with tuberculosis. The major CT manifestations were a cluster of nodules or masses located within 2 cm below the pleura and distributed along the bronchi. A total of nine patients had primary lesions with diameters of 1.1–2.0 cm and 12 patients had satellite lesions with diameters of 0.1–1.0 cm. Regarding treatment, 5 patients underwent surgical monotherapy, 12 patients underwent antifungal monotherapy and three patients received surgery in combination with antifungal therapy. HE staining indicated that Cryptococcus neoformans was engulfed by macrophages, which were surrounded by massive infiltrating lymphocytes and a large amount of fibrous tissue, which formed multinucleated macrophages or granulomas. Periodic acid-Schiff staining was positive and acid fast staining was negative. In conclusion, comparison of CT signs with the pathological manifestation of pulmonary cryptococcosis with multiple nodules or masses indicated that the pathological results may explain certain imaging signs. Combination of CT and pathological examination may provide a deeper understanding of this disease and improve the accuracy of its diagnosis. more...
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- 2018
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18. Clinical features and radiological characteristics of pulmonary cryptococcosis
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Jie Zhang, Jia Li, Hui Deng, Xinying Xue, Dongxu Wang, and Lei Pan
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Male ,Medicine (General) ,Antifungal Agents ,Clinical Research Reports ,Biopsy ,Computed tomography ,Biochemistry ,Ground-glass opacity ,miliary nodules ,0302 clinical medicine ,Medicine ,030212 general & internal medicine ,Child ,Pneumonectomy ,Lung ,multiple nodules/masses ,medicine.diagnostic_test ,General Medicine ,Cryptococcosis ,Middle Aged ,Radiological weapon ,Child, Preschool ,Female ,Radiology ,medicine.symptom ,Antifungal ,Adult ,enlarged mediastinal lymph nodes ,medicine.medical_specialty ,Adolescent ,medicine.drug_class ,single nodule/mass ,03 medical and health sciences ,Young Adult ,R5-920 ,Humans ,Pulmonary cryptococcosis ,Retrospective Studies ,Lung Diseases, Fungal ,business.industry ,Biochemistry (medical) ,Infant, Newborn ,Infant ,chest computed tomography ,Retrospective cohort study ,Nodule (medicine) ,Cell Biology ,medicine.disease ,030228 respiratory system ,business ,Tomography, X-Ray Computed - Abstract
Objective Diagnosis of pulmonary cryptococcosis is difficult. In this study, we examined the clinical and radiological features that increase the diagnostic accuracy for pulmonary cryptococcosis. Methods This retrospective study included clinical data from 68 patients with pulmonary cryptococcosis from 2012 to 2016 in 3 tertiary hospitals. Results Among the 68 patients, 39 (57.35%) had no complications, 39 (57.35%) had clinical symptoms, 6 (8.82%) had a history of occupational exposure, 27 (39.71%) had a single nodule/mass (the most common type of pulmonary cryptococcosis) on chest computed tomography images, 21 (30.88%) had multiple nodules/masses, 16 (23.53%) had ground glass opacity with or without nodules, 2 (2.94%) had miliary nodules, and 2 (2.94%) had enlarged mediastinal lymph nodes. Fifty-three (77.94%) patients had lesions with irregular margins, 33 (48.53%) had spiculated lesions, 32 (47.06%) had air bronchograms, 9 (13.24%) had cavities, and 4 (5.88%) had calcifications. Twenty-four patients underwent surgery, 35 received antifungal treatment, and 9 received both treatments. Conclusion The clinical features and computed tomography signs found in this study are not specific for a diagnosis of pulmonary cryptococcosis. Therefore, an increased awareness of pulmonary cryptococcosis is needed among clinicians. more...
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- 2018
19. IgG4-related disease of pulmonary artery causing pulmonary hypertension
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Peiliang Gao, Xinying Xue, Xi-Qi Xu, Yali Xu, Zhi-Cheng Jing, Xiaoyan Yao, Deng Hui, Jin Qian, Yunlong Yue, Xin Jiang, Yong Liu, Sheng Zhao, Xiaorong Ma, Lei Pan, and Yong Wang
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Adult ,Male ,medicine.medical_specialty ,Cardiac Catheterization ,Multifunction cardiogram ,Computed Tomography Angiography ,medicine.medical_treatment ,Hypertension, Pulmonary ,Observational Study ,positron emission tomography/computed tomography ,030204 cardiovascular system & hematology ,Pulmonary Artery ,Autoimmune Diseases ,Diagnosis, Differential ,03 medical and health sciences ,0302 clinical medicine ,medicine.artery ,Positron Emission Tomography Computed Tomography ,parasitic diseases ,pulmonary hypertension ,Pulmonary angiography ,Medicine ,Humans ,Thrombus ,IgG4-related disease ,Cyclophosphamide ,Glucocorticoids ,Computed tomography angiography ,Cardiac catheterization ,Retrospective Studies ,030203 arthritis & rheumatology ,medicine.diagnostic_test ,business.industry ,fungi ,General Medicine ,Middle Aged ,medicine.disease ,Pulmonary hypertension ,Magnetic Resonance Imaging ,Immunoglobulin G ,Pulmonary artery ,Radiology ,business ,Rituximab ,Research Article - Abstract
IgG4-related disease (IgG4-RD) is recognized as an immune-mediated condition with pathology features of lymphoplasmacytic infiltrate, storiform fibrosis, and obliterative phlebitis, accompanied with or without elevated serum IgG4 concentrations. However, few of pulmonary artery IgG4-RD causing pulmonary hypertension (PH) was reported. The medical records of 3 patients with pulmonary artery IgG4-RD inducing PH were analyzed retrospectively. Imaging findings demonstrated that the lesions of 3 patients located in pulmonary artery, which were initially diagnosed as pulmonary thrombus or malignant tumor. Computed tomography pulmonary angiography (CTPA), ultrasonic cardiogram, and positron emission tomography/computed tomography (PET/CT) didn’t support the diagnosis of pulmonary thrombus or malignant tumor. Right heart catheterization (RHC) showed definite PH. Biopsy by right heart catheterization in 2 patients or pneumonectomy in 1 patient confirmed the diagnosis as IgG4-RD. Treated with glucocorticoids and cyclophosphamide or rituximab, 2 patients’ IgG4 concentrations declined sharply and the lesions shrunk gradually. Another patient treated with glucocorticoids died of heart failure. IgG4-RD involved pulmonary artery causing PH was rare. A high index of awareness of this disease is required for early diagnosis and treatment. PET/CT might be a valuable approach to distinguish pulmonary artery IgG4-RD from pulmonary thrombus and malignant tumor. more...
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- 2018
20. Comparison of pyogenic liver abscesses caused by hypermucoviscous Klebsiella pneumoniae and non-Klebsiella pneumoniae pathogens in Beijing: A retrospective analysis
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Kaifei Wang, Dingxia Shen, Jing Wang, Xinying Xue, and Yan Yan
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Adult ,Male ,China ,medicine.medical_specialty ,Pathology ,Adolescent ,Klebsiella pneumoniae ,Biochemistry ,Gastroenterology ,Serology ,Diabetes Complications ,Hospitals, Urban ,Risk Factors ,Internal medicine ,Diabetes mellitus ,Diabetes Mellitus ,medicine ,Retrospective analysis ,Humans ,Aged ,Retrospective Studies ,Ultrasonography ,Aged, 80 and over ,biology ,business.industry ,Incidence (epidemiology) ,Biochemistry (medical) ,Cell Biology ,General Medicine ,Middle Aged ,medicine.disease ,biology.organism_classification ,Bacterial Typing Techniques ,Klebsiella Infections ,Radiography ,Liver ,Liver Abscess, Pyogenic ,Cohort ,Female ,business ,Abdominal surgery ,Liver abscess - Abstract
Objective To perform a retrospective comparison of the clinical and radiological features of Klebsiella pneumoniae (KP)-associated and non-KP-associated pyogenic liver abscesses (PLA) in Chinese patients. Methods Patients with confirmed diagnoses of bacterial liver abscess at three Beijing hospitals were enrolled. Clinical isolates from liver abscesses were used to determine serology and expression of hypermucoviscosity genes. Basic clinical, ultrasonographic (US) and computed tomography (CT) data were recorded and compared between patients with KP- and non-KP-associated PLA. Results A total of 101 (77.10%) and 30 (22.90%) cases were due to KP and non-KP pathogens, respectively. Compared with the non-KP cohort, the KP cohort demonstrated a significantly higher incidence of underlying diabetes mellitus, and more gas-forming abscesses, as demonstrated by US and CT examinations. Prior abdominal surgery or chemoradiation therapy was significantly associated with non-KP cases. The non-KP group had a higher chance of a clear edge, compared with the KP group, on pre-contrast CT images. Conclusion KP and non-KP-associated PLA have distinctive risk factors and unique US and CT features, in Chinese patients. more...
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- 2013
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21. Exogenous pulmonary surfactant for acute respiratory distress syndrome in adults: A systematic review and meta-analysis
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Jianxin Wang, Lina Zhang, Xinying Xue, and Junping Sun
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Cancer Research ,medicine.medical_specialty ,Pathology ,ARDS ,pulmonary surfactant ,APACHE II ,business.industry ,Subgroup analysis ,Articles ,General Medicine ,Publication bias ,acute respiratory distress syndrome ,Pulmonary compliance ,medicine.disease ,mortality ,law.invention ,meta-analysis ,Systematic review ,Immunology and Microbiology (miscellaneous) ,Randomized controlled trial ,law ,Internal medicine ,Meta-analysis ,medicine ,business - Abstract
Acute respiratory distress syndrome (ARDS) is often characterized by reduced lung compliance, which suggests dysfunction of the endogenous surfactant system. The effectiveness of exogenous surfactants as replacements for the endogenous system in the treatment of ARDS in adults was assessed. Randomized controlled trials from Medline (1950-2011), Embase (1989-2011), the Cochrane Database of Systematic Reviews and the Cochrane Central Register of Controlled Trials (1994-2011) were analyzed. Two reviewers identified trials for inclusion and the results of included trials were quantitatively pooled with a fixed-effects model. Seven trials (2,144 patients) with good methodological quality were included in the analysis. Pulmonary surfactant treatment was not associated with reduced mortality [relative risk (RR), 1.00; 95% confidence interval (CI) 0.89-1.12]. Subgroup analysis revealed no reduced mortality for various surfactant types. Heterogeneity was not significant in the primary outcome analysis (I(2)=0%). There was no evidence of publication bias. Oxygenation, ventilation-free days, duration of ventilation and APACHE II scores did not undergo pooled analysis due to insufficient data. Exogenous surfactant did not reduce mortality in adults with ARDS in our meta-analysis, and we cannot accurately define whether exogenous surfactant has an effect on oxygenation from the included studies. more...
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- 2012
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22. The relationship of Transforming Growth Factor-β and lung cancer
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Lei Pan, Kaifei Wang, Ting Ao, Mingyue Zhang, Xuefeng Zang, Mingming Meng, Xiaoyan Yao, Xinying Xue, Jianxin Wang, Yuxia Liu, and Guigen Teng
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business.industry ,Cancer research ,medicine ,General Medicine ,Lung cancer ,medicine.disease ,business ,Transforming growth factor - Published
- 2016
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23. SchA-p85-FAK complex dictates isoform-specific activation of Akt2 and subsequent PCBP1-mediated post-transcriptional regulation of TGFβ-mediated epithelial to mesenchymal transition in human lung cancer cell line A549
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Guigen Teng, Yali Xu, Jianxin Wang, Yong Wang, Jinghui Zhang, Xinying Xue, Lei Pan, Yuxia Liu, Xuefeng Zang, Kaifei Wang, and Xin Wang
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Epithelial-Mesenchymal Transition ,Lung Neoplasms ,Biology ,Heterogeneous-Nuclear Ribonucleoproteins ,Focal adhesion ,Downregulation and upregulation ,Transforming Growth Factor beta ,Cell Line, Tumor ,Humans ,Protein Isoforms ,Epithelial–mesenchymal transition ,Phosphorylation ,PI3K/AKT/mTOR pathway ,A549 cell ,Signal transducing adaptor protein ,RNA-Binding Proteins ,General Medicine ,Cell biology ,Class Ia Phosphatidylinositol 3-Kinase ,DNA-Binding Proteins ,Cell culture ,Focal Adhesion Protein-Tyrosine Kinases ,embryonic structures ,Cancer research ,Proto-Oncogene Proteins c-akt - Abstract
A post-transcriptional pathway by which TGF-β modulates expression of specific proteins, Disabled-2 (Dab2) and Interleukin-like EMT Inducer (ILEI), inherent to epithelial to mesenchymal transition (EMT) in murine epithelial cells through Akt2-mediated phosphorylation of poly r(C) binding protein (PCBP1), has been previously elucidated. The aims of the current study were to determine if the same mechanism is operative in the non-small cell lung cancer (NSCLC) cell line, A549, and to delineate the underlying mechanism. Steady-state transcript and protein expression levels of Dab2 and ILEI were examined in A549 cells treated with TGF-β for up to 48 h. Induction of translational de-repression in this model was quantified by polysomal fractionation followed by qRT-PCR. The underlying mechanism of isoform-specific activation of Akt2 was elucidated through a combination of co-immunoprecipitation studies. TGF-β induced EMT in A549 cells concomitant with translational upregulation of Dab2 and ILEI proteins through isoform-specific activation of Akt2 followed by phosphorylation of PCBP1 at serine-43. Our experiments further elucidated that the adaptor protein SchA is phosphorylated at tyrosine residues following TGF-β treatment, which initiated a signaling cascade resulting in the sequential recruitment of p85 subunit of PI3K and focal adhesion kinase (FAK). The SchA-FAK-p85 complex subsequently selectively recruited and activated Akt2, not Akt1. Inhibition of the p85 subunit through phosphorylated 1257 peptide completely attenuated EMT in these cells. We have defined the underlying mechanism responsible for isoform-specific recruitment and activation of Akt2, not Akt1, during TGF-β-mediated EMT in A549 cells. Inhibition of the formation of this complex thus represents an important and novel therapeutic target in metastatic lung carcinoma. more...
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- 2013
24. Diagnosis of multiple primary lung cancer: a systematic review
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Lei Pan, Yong Wang, Pei-lan Wang, Mingyue Zhang, Xinying Xue, Kaifei Wang, Jianxin Wang, and Yuxia Liu
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Oncology ,medicine.medical_specialty ,Lung Neoplasms ,business.industry ,Biochemistry (medical) ,Cancer ,Cell Biology ,General Medicine ,Newly diagnosed ,medicine.disease ,Biochemistry ,Metastasis ,ErbB Receptors ,Neoplasms, Multiple Primary ,Proto-Oncogene Proteins p21(ras) ,Internal medicine ,Carcinoma, Non-Small-Cell Lung ,Proto-Oncogene Proteins ,medicine ,ras Proteins ,Humans ,In patient ,Differential diagnosis ,Lung cancer ,business - Abstract
A substantial percentage (8%) of all newly diagnosed cancer cases are in patients with previous tumours, with a similar trend in lung cancer. Cases of multiple primary lung cancer (MPLC) are increasing worldwide, due to improved diagnostic and surveillance mechanisms and the ageing population. Diagnosis of MPLC is complicated by difficulties in distinguishing it from lung cancer metastasis. Clinicopathological assessment, diagnosis and management have evolved, but remain severely limited by the lack of robust and dependable molecular markers for the differential diagnosis of metastasis and MPLC. This systematic review evaluates diagnostic criteria for MPLC, and the subsequent management and success rates. The incorporation of molecular biology techniques into the diagnostic process for MPLC is also discussed. more...
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- 2013
25. Pulmonary arterial hypertension and microRNAs--an ever-growing partnership
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Jie Zhang, Lei Pan, Pei-lan Wang, Yong Wang, Jianxin Wang, Yuxia Liu, Shuyang Zhang, Kaifei Wang, Xinying Xue, Jing Wang, and Xuefeng Zang
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Male ,Pathology ,medicine.medical_specialty ,Hypertension, Pulmonary ,Pulmonary Artery ,Bioinformatics ,Asymptomatic ,microRNA ,medicine ,Gene silencing ,Animals ,Humans ,Familial Primary Pulmonary Hypertension ,Pulmonary wedge pressure ,Aged ,Cell Proliferation ,Effector ,business.industry ,General Medicine ,medicine.disease ,Phenotype ,Pulmonary hypertension ,Pathophysiology ,MicroRNAs ,Gene Expression Regulation ,Disease Progression ,medicine.symptom ,business - Abstract
Pulmonary arterial hypertension (PAH) is a debilitating condition with progressive remodeling of the pulmonary resistance vessels. PAH is characterized by multifocal, polyclonal lesions inhabited by cells that underwent phenotypic transition, resulting in altered cell proliferation and contractility, ultimately resulting in increased vascular resistance. Diagnosis of PAH is confounded by the fact that it is largely asymptomatic in the initial stages. In fact, idiopathic PAH patients >65 years of age cannot be diagnosed hemodynamically due to high pulmonary capillary wedge pressure. This highlights the need for defining more robust molecular biomarkers for PAH diagnosis and progression. Recent studies have indicated that microRNAs (miRNAs), a class of small noncoding RNAs that regulate gene expression, play a discrete role in vascular inflammation and in the etiology of cardiovascular pathologies inclusive of PAH and can potentially serve as diagnostic biomarkers. However, a cohesive understanding of global miRNA-mediated molecular events that control pulmonary vasculature plasticity is lacking which, if addressed systematically, can lead to detailed elucidation of the downstream cellular pathways that are affected by activation/silencing of silenced cognate transcripts. In turn, this can lead to not only robust biomarkers, but also to novel therapeutic strategies targeting more upstream regulators than the existing ones targeting more downstream effectors. The current review aims to provide a summary understanding of PAH, its associated pathophysiology, current knowledge of the role of miRNAs in PAH, and identifies grey areas that need further research for successful bench-to-bedside transition of these exciting new discoveries. more...
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- 2013
26. Plasmid-mediated quinolone resistance--current knowledge and future perspectives
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Bing Yang, Kaifei Wang, Jing Wang, Xi-zhou Guan, Na Wang, Hong Jiang, Yong Wang, Lei Pan, Jianxin Wang, Yuxia Liu, Lina Zhang, and Xinying Xue
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medicine.drug_class ,Drug resistance ,Pharmacology ,Gene mutation ,Quinolones ,Biochemistry ,Bacterial genetics ,Microbiology ,Plasmid ,Drug Resistance, Bacterial ,medicine ,Animals ,Humans ,biology ,business.industry ,Biochemistry (medical) ,Cell Biology ,General Medicine ,Bacterial Infections ,Quinolone ,biology.organism_classification ,Antimicrobial ,Anti-Bacterial Agents ,Genes, Bacterial ,Efflux ,business ,Bacteria ,Plasmids - Abstract
Quinolones are a group of antimicrobial agents that were serendipitously discovered as byproducts of the synthesis of chloroquine. Chemical modifications, such as the addition of fluorine or piperazine, resulted in the synthesis of third- and fourth-generation fluoroquinolones, with broad-spectrum antimicrobial actions against aerobic or anaerobic, Gram-positive or Gram-negative bacteria. The efficacy and consequent widespread use of quinolones and fluoroquinolones has led to a steady global increase in resistance, mediated via gene mutations, alterations in efflux or cell membranes and plasmid-conferred resistance. The first plasmid-mediated quinolone resistance gene, qnrA1, was detected in 1998. Since then, many other genes have been identified and the underlying mechanisms of resistance have been elucidated. This review provides an overview of quinolone resistance, with particular emphasis on plasmid-mediated resistance. more...
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- 2013
27. Endobronchial tuberculosis: an overview
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Jing Wang, Qingliang Xue, Na Wang, and Xinying Xue
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Microbiology (medical) ,medicine.medical_specialty ,Tuberculosis ,Antitubercular Agents ,Disease ,Constriction, Pathologic ,Mycobacterium tuberculosis ,Bronchoscopy ,medicine ,Humans ,Intensive care medicine ,Tuberculosis, Pulmonary ,Bacteriological Techniques ,biology ,medicine.diagnostic_test ,business.industry ,Incidence ,Bronchial Diseases ,General Medicine ,medicine.disease ,biology.organism_classification ,Surgery ,Chronic infection ,Infectious Diseases ,Early Diagnosis ,Etiology ,Sputum ,Actinomycosis ,Radiography, Thoracic ,medicine.symptom ,business - Abstract
Endobronchial tuberculosis (EBTB), of which the incidence has been increasing in recent years, is a special type of pulmonary tuberculosis. The endobronchial tuberculose focuses often injure the tracheobronchial wall and lead to tracheobronchial stenosis. The tracheobronchial stenosis may cause intractable tuberculosis and make patients become chronic infection sources of tuberculosis, or may even cause pulmonary complications and result in death. The etiological confirmation of Mycobacterium tuberculosis is most substantial for diagnosis. However, because the positive rate of acid-fast bacillus staining for sputum smears is low and the clinical and radiological findings are usually nondistinctive, the diagnosis of EBTB is often mistaken and delayed. For early diagnosis, a high index of awareness of this disease is required and the bronchoscopy should be performed as soon as possible in suspected patients. The eradication of Mycobacterium tuberculosis and the prevention of tracheobronchial stenosis are two most substantial treatment goals. To get treatment goals, the diagnosis must be established early and aggressive treatments must be performed before the disease progresses too far. more...
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- 2011
28. Doxorubicin nanobubble for combining ultrasonography and targeted chemotherapy of rabbit with VX2 liver tumor
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Mingming Meng, Xiang-Chun Lin, Jing Wu, Kuiliang Liu, Hong Liu, Hui Su, Cang-hai Wang, Jie Gao, Yadan Wang, Xuefeng Zang, Xinying Xue, Chongchong Wu, and Xuan Zhou
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0301 basic medicine ,medicine.medical_specialty ,Cancer Research ,Liver tumor ,Proliferation index ,02 engineering and technology ,macromolecular substances ,03 medical and health sciences ,chemistry.chemical_compound ,Drug Delivery Systems ,Liver Neoplasms, Experimental ,In vivo ,Ultrasound ,medicine ,Tumor Cells, Cultured ,polycyclic compounds ,Animals ,Doxorubicin ,Ultrasonics ,VX2 liver tumor ,Drug Carriers ,Antibiotics, Antineoplastic ,business.industry ,technology, industry, and agriculture ,General Medicine ,021001 nanoscience & nanotechnology ,medicine.disease ,Xenograft Model Antitumor Assays ,Surgery ,Treatment ,PLGA ,030104 developmental biology ,chemistry ,Drug delivery ,Nanoparticles ,Original Article ,Doxorubicin nanobubble (DOX-NB) ,Rabbits ,0210 nano-technology ,business ,Drug carrier ,Biomedical engineering ,medicine.drug - Abstract
A new class of multifunctional nanobubble using poly(lactic-co-glycolic acid) (PLGA) has been developed as ultrasound imaging contrast agents, doxorubicin carriers, and enhancers of ultrasound-mediated drug delivery. The doxorubicin nanobubble (DOX-NB) wrapping carbon tetrafluoride gas was prepared with double emulsion method. We evaluated the enhanced ultrasonic function of the DOX-NB in vivo; its antitumor function was confirmed. The diameter of the prepared bubble was 500 nm, and the potential was −23 mV. The drug loading and encapsulation efficiency of the bubble were 78.6 and 7.4 %, respectively. Therefore, the DOX-NB greatly enhanced ultrasound imaging in vivo. Ultrasound combined with DOX-NB had significant antitumor effect. Compared with other groups, the tumor growth rate and the proliferation index were the lowest while the survival rate and apoptosis index were the highest. more...
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