1. Systematic discovery of new genes in the Saccharomyces cerevisiae genome.
- Author
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Kessler MM, Zeng Q, Hogan S, Cook R, Morales AJ, and Cottarel G
- Subjects
- Amino Acid Sequence genetics, Animals, Caenorhabditis elegans Proteins genetics, Cattle, Chromosome Mapping methods, Chromosomes, Fungal genetics, Conserved Sequence genetics, Drosophila Proteins genetics, Humans, Mice, Molecular Sequence Data, Open Reading Frames genetics, Proteins genetics, Sequence Homology, Amino Acid, Genes, Fungal genetics, Genome, Fungal, Saccharomyces cerevisiae genetics
- Abstract
We used genome-wide comparative analysis of predicted protein sequences to identify many novel small genes, named smORFs for small open reading frames, within the budding yeast genome. Further analysis of 117 of these new genes showed that 84 are transcribed. We extended our analysis of one smORF conserved from yeast to human. This investigation provides an updated and comprehensive annotation of the yeast genome, validates additional concepts in the study of genomes in silico, and increases the expected numbers of coding sequences in a genome with the corresponding impact on future functional genomics and proteomics studies. more...
- Published
- 2003
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