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Foreign genes were expressed in liver and skin cells of live mice by using a new apparatus to accelerate DNA-coated microprojectiles into tissues. After introduction of a plasmid in which the firefly luciferase gene was controlled by the human beta-actin promoter, luciferase activity was detectable for up to 14 days in mouse tissues (skin and liver). In situ hybridization histochemistry revealed that microprojectiles penetrated through multiple cell layers without evidence of tissue injury and that 10-20% of the cells in the bombarded area expressed the foreign gene. An advantage of the new design is that internal organs, such as liver, can be transfected without subjecting the tissue to a vacuum. This procedure potentially is applicable to a wide variety of tissues and cell types for studies of transcriptional control elements and for expression of foreign proteins in intact animals.

The article presents a research study on the signal transduction and activator of transcription 6's (STAT6) DNA interaction using JNJ7777120 (JNJ), and JNJ10191584 (VUF 6002). It states the measurement of the DNA-binding by electrophoretic mobility shift assay (EMSA). It states that STAT1/STAT6 balance should be considered for being adjusted by antihistamines.