1. Persisting roadblocks in arthropod monitoring using non-destructive metabarcoding from collection media of passive traps.
- Author
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Sire, Lucas, Yáñez, Paul Schmidt, Bézier, Annie, Courtial, Béatrice, Mbedi, Susan, Sparmann, Sarah, Larrieu, Laurent, Rougerie, Rodolphe, Bouget, Christophe, Monaghan, Michael T., Herniou, Elisabeth A., and Lopez-Vaamonde, Carlos
- Subjects
GENETIC barcoding ,ARTHROPODA ,FOREST declines ,SILVER fir ,FOREST management ,ETHANOL - Abstract
Background. Broad-scale monitoring of arthropods is often carried out with passive traps (e.g., Malaise traps) that can collect thousands of specimens per sample. The identification of individual specimens requires time and taxonomic expertise, limiting the geographical and temporal scale of research and monitoring studies. DNA metabarcoding of bulk-sample homogenates has been found to be faster, efficient and reliable, but the destruction of samples prevents a posteriori validation of species occurrences and relative abundances. Non-destructive metabarcoding of DNA extracted from collection medium has been applied in a limited number of studies, but further tests of efficiency are required with different trap types and collection media to assess the consistency of the method. Methods. We quantified the detection rate of arthropod species when applying nondestructive DNA metabarcoding with a short (127-bp) fragment of mitochondrial COI on two combinations of passive traps and collection media: (1) water with monopropylene glycol (H
2 O-MPG) used in window-flight traps (WFT, 53 in total); (2) ethanol with monopropylene glycol (EtOH-MPG) used in Malaise traps (MT, 27 in total). We then compared our results with those obtained for the same samples using morphological identification (for WFTs) or destructive metabarcoding of bulk homogenate (for MTs). This comparison was applied as part of a larger study of arthropod species richness in silver fir (Abies alba Mill., 1759) stands across a range of climate-induced tree dieback levels and forest management strategies. Results. Of the 53 H2 O-MPG samples from WFTs, 16 produced no metabarcoding results, while the remaining 37 samples yielded 77 arthropod MOTUs in total, of [ABSTRACT FROM AUTHOR]- Published
- 2023
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