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The genus Jasminum L., of the family Oleaceae, includes many species occurring in the wild, or cultivated worldwide. A preliminary investigation based on inter-simple sequence repeats (ISSR) was performed to assess the genetic diversity among 28 accessions, representing nine species of Jasminum from various regions, representing a range of altitudes in Pakistan. A total of 21 ISSR primers were used, which produced 570 amplified bands of different sizes, with a mean polymorphic band percentage of 98.26%. The maximum resolving power, polymorphism information content, and index values of the ISSR markers recorded for primers 6, 16, and 19 were 0.40, 12.32, and 24.21, respectively. Based on the data of the ISSR markers, the resulting UPGMA dendrogram with the Jaccard coefficient divided the 28 accessions into two main clades. At the species level, the highest values for Shannon's information index, polymorphism percentage, effective allele number, Nei's genetic variations, and genetic unbiased diversity were found in Jasminum sambac L. and J. humile L., while the lowest were observed in J. mesnyi Hance and J. nitidum Skan. Based on Nei's unbiased genetic identity pairwise population matrix, the maximum identity (0.804) was observed between J. elongatum Willd and J. multiflorum (Burm. f.) Andrews, and the lowest (0.566) between J. nitidum Skan. and J. azoricum L. Molecular variance analysis displayed a genetic variation of 79% among the nine populations. The study was aimed to established genetic diversity in Jasminum species using ISSR markers. With the help of this technique, we were able to establish immense intra- and interspecific diversity across the Jasminum species.

The present study was carried out to characterize the exotic olive cultivars on morphological as well as phenological basis and to assess their adaptability in Pothwar (Chakwal area) climatic condition of Pakistan. Majority of the parameters indicated significant variability between the cultivars and positive significant correlation were obtained between the parameters. Cultivars Ottobratica and Arbequina could be used for pollination purpose against maximum cultivars due to the longest flowering duration. Maximum fruit set percentage was recorded in cultivar Arbequina (5.49%) while poor fruit set was observed in cultivar Koroneiki (1.15%) with the mean of 2.66 for all cultivars. Maximum yield per plant was obtained from the cultivar Coratina (22kg) while minimum was recorded from the cultivar Leccino (2.5kg) with mean of 10.54kg. It is concluded from the correlation that bold size cultivars like Coratina, Gemlik, Jerboui and Nabali could be recommended for projection of olive industry in Pakistan under the climatic condition of Chakwal as fruit set percentage has positive correlation with fruit weight. The grouping of above mentioned cultivars in bi-plot on the basis of oil percentage, fruit set percentage and yield per tree also confirmed the recommendation of cultivars. Overall the trait of final fruit set percentage showed the greatest variability among all the traits among all the cultivars. Five cultivars included Coratina, Gemlik, Nabali, Picholine and Jerboui were most suitable for oil and yield production under the climatic condition of Pothwar, Pakistan. [ABSTRACT FROM AUTHOR]

The genus Jasminum L., of the family Oleaceae, includes many species occurring in the wild, or cultivated worldwide. A preliminary investigation based on inter-simple sequence repeats (ISSR) was performed to assess the genetic diversity among 28 accessions, representing nine species of Jasminum from various regions, representing a range of altitudes in Pakistan. A total of 21 ISSR primers were used, which produced 570 amplified bands of different sizes, with a mean polymorphic band percentage of 98.26%. The maximum resolving power, polymorphism information content, and index values of the ISSR markers recorded for primers 6, 16, and 19 were 0.40, 12.32, and 24.21, respectively. Based on the data of the ISSR markers, the resulting UPGMA dendrogram with the Jaccard coefficient divided the 28 accessions into two main clades. At the species level, the highest values for Shannon's information index, polymorphism percentage, effective allele number, Nei's genetic variations, and genetic unbiased diversity were found in Jasminum sambac L. and J. humile L., while the lowest were observed in J. mesnyi Hance and J. nitidum Skan. Based on Nei's unbiased genetic identity pairwise population matrix, the maximum identity (0.804) was observed between J. elongatum Willd and J. multiflorum (Burm. f.) Andrews, and the lowest (0.566) between J. nitidum Skan. and J. azoricum L. Molecular variance analysis displayed a genetic variation of 79% among the nine populations. The study was aimed to established genetic diversity in Jasminum species using ISSR markers. With the help of this technique, we were able to establish immense intra- and interspecific diversity across the Jasminum species. [ABSTRACT FROM AUTHOR]