10 results on '"Mwacharo, Joram M"'
Search Results
2. The wild species genome ancestry of domestic chickens
- Author
-
Lawal, Raman Akinyanju, Martin, Simon H., Vanmechelen, Koen, Vereijken, Addie, Silva, Pradeepa, Al-Atiyat, Raed Mahmoud, Aljumaah, Riyadh Salah, Mwacharo, Joram M., Wu, Dong-Dong, Zhang, Ya-Ping, Hocking, Paul M., Smith, Jacqueline, Wragg, David, and Hanotte, Olivier
- Published
- 2020
- Full Text
- View/download PDF
3. Whole-genome resequencing of worldwide wild and domestic sheep elucidates genetic diversity, introgression, and agronomically important loci
- Author
-
Lv, Feng-Hua, Cao, Yin-Hong, Liu, Guang-Jian, Luo, Ling-Yun, Lu, Ran, Liu, Ming-Jun, Li, Wen-Rong, Zhou, Ping, Wang, Xin-Hua, Shen, Min, Gao, Lei, Yang, Jing-Quan, Yang, Hua, Yang, Yong-Lin, Liu, Chang-Bin, Wan, Peng-Cheng, Zhang, Yun-Sheng, Pi, Wen-Hui, Ren, Yan-Ling, Shen, Zhi-Qiang, Wang, Feng, Wang, Yu-Tao, Li, Jin-Quan, Salehian-Dehkordi, Hosein, Hehua, Eer, Liu, Yong-Gang, Chen, Jian-Fei, Wang, Jian-Kui, Deng, Xue-Mei, Esmailizadeh, Ali, Dehghani-Qanatqestani, Mostafa, Charati, Hadi, Nosrati, Maryam, Štěpánek, Ondřej, Rushdi, Hossam E., Olsaker, Ingrid, Curik, Ino, Gorkhali, Neena A., Paiva, Samuel R., Caetano, Alexandre R., Ciani, Elena, Amills, Marcel, Weimann, Christina, Erhardt, Georg, Amane, Agraw, Mwacharo, Joram M., Han, Jian-Lin, Hanotte, Olivier, Periasamy, Kathiravan, Johansson, Anna M., Hallsson, Jón H., Kantanen, Juha, Coltman, David W., Bruford, Michael W., Lenstra, Johannes A., Li, Meng-Hua, Nowick, Katja, National Natural Science Foundation of China, National Key Research and Development Program (China), Chinese Academy of Sciences, FENG-HUA LV, China Agricultural University, China, YIN-HONG CAO, Chinese Academy of Sciences (CAS), China, GUANG-JIAN LIU, Novogene Bioinformatics Institute, China, LING-YUN LUO, China Agricultural University, China, RAN LU, China Agricultural University, China, MING-JUN LIU, Xinjiang Academy of Animal Science, China, WEN-RONG LI, Xinjiang Academy of Animal Science, China, PING ZHOU, Xinjiang Academy of Agricultural and Reclamation Sciences, China, XIN-HUA WANG, Xinjiang Academy of Agricultural and Reclamation Sciences, China, MIN SHEN, Xinjiang Academy of Agricultural and Reclamation Sciences, China, LEI GAO, Xinjiang Academy of Agricultural and Reclamation Sciences, China, JING-QUAN YANG, Xinjiang Academy of Agricultural and Reclamation Sciences, China, HUA YANG, Xinjiang Academy of Agricultural and Reclamation Sciences, China, YONG-LIN YANG, Xinjiang Academy of Agricultural and Reclamation Sciences, China, CHANG-BIN LIU, Xinjiang Academy of Agricultural and Reclamation Sciences, China, PENG-CHENG WAN, Xinjiang Academy of Agricultural and Reclamation Sciences, China, YUN-SHENG ZHANG, Xinjiang Academy of Agricultural and Reclamation Sciences, China, WEN-HUI PI, Xinjiang Academy of Agricultural and Reclamation Sciences, China, YAN-LING REN, Shandong Binzhou Academy of Animal Science and Veterinary Medicine, China, ZHI-QIANG SHEN, Shandong Binzhou Academy of Animal Science and Veterinary Medicine, China, FENG WANG, Nanjing Agricultural University, China, YU-TAO WANG, Kashi University, China, JIN-QUAN LI, Inner Mongolia Agricultural University, China, HOSEIN SALEHIAN-DEHKORDI, Chinese Academy of Sciences (CAS), China, EER HEHUA, Ningxia Academy of Agriculture and Forestry Sciences, China, YONG-GANG LIU, Yunnan Agricultural University, China, JIAN-FEI CHEN, China Agricultural University, China, JIAN-KUI WANG, China Agricultural University, China, XUE-MEI DENG, China Agricultural University, China, ALI ESMAILIZADEH, Shahid Bahonar University of Kerman, Iran, MOSTAFA DEHGHANI-QANATQESTANI, Shahid Bahonar University of Kerman, Iran, HADI CHARATI, Shahid Bahonar University of Kerman, Iran, MARYAM NOSRATI, Payame Noor University, Iran, ONDREJ STEPANEK, State Veterinary Institute Jihlava, Czech Republic, HOSSAM E. RUSHDI, Cairo University, Egypt, INGRID OLSAKER, Norwegian University of Life Sciences, Norway, INO CURIK, University of Zagreb, Croatia, NEENA A. GORKHALI, Nepal Agriculture Research Council (NARC), Nepal, SAMUEL REZENDE PAIVA, Cenargen, ALEXANDRE RODRIGUES CAETANO, Cenargen, ELENA CIANI, Universita degli Studi di Bari Aldo 24 Moro, Italy, MARCEL AMILLS, Campus de la Universitat Autonoma de Barcelona, Spain, CHRISTINA WEIMANN, Justus-Liebig-University Giessen, Germany, GEORG ERHARDT, Justus-Liebig-University Giessen, Germany, AGRAW AMANE, Addis Ababa University, Ethiopia, JORAM M. MWACHARO, International Centre for Agricultural Research in the Dry Areas (ICARDA), Ethiopia, JIAN-LIN HAN, Chinese Academy of Agricultural Sciences (CAAS), China, OLIVIER HANOTTE, International Livestock Research Institute, Ethiopia, KATHIRAVAN PERIASAMY, International Atomic Energy Agency (IAEA), Austria, ANNA M. JOHANSSON, Swedish University of Agricultural Sciences, Sweden, JON H. HALLSSON, Agricultural University of Iceland, Iceland, JUHA KANTANEN, Natural Resources Institute Finland (Luke), Finland, DAVID W. COLTMAN, University of Alberta, Edmonton, Canada, MICHAEL W. BRUFORD, Cardiff University, United Kingdom, JOHANNES A. LENSTRA, Utrecht University, The Netherlands, and MENG-HUA LI, China Agricultural University, China.
- Subjects
Asia ,Genome ,Sheep ,Whole-genome sequences ,Genetic Variation ,Genetic selection ,Sequence Analysis, DNA ,Iran ,Polymorphism, Single Nucleotide ,Genetic diversity ,whole-genome sequences ,genetic diversity ,adaptive introgression ,genetic selection ,agronomic traits ,migration ,Europe ,Adaptive introgression ,Genetics ,Animals ,Agronomic traits ,Molecular Biology ,Sheep, Domestic ,Ecology, Evolution, Behavior and Systematics ,Migration - Abstract
Domestic sheep and their wild relatives harbor substantial genetic variants that can form the backbone of molecular breeding, but their genome landscapes remain understudied. Here, we present a comprehensive genome resource for wild ovine species, landraces and improved breeds of domestic sheep, comprising high-coverage (∼16.10×) whole genomes of 810 samples from 7 wild species and 158 diverse domestic populations. We detected, in total, ∼121.2 million single nucleotide polymorphisms, ∼61 million of which are novel. Some display significant (P < 0.001) differences in frequency between wild and domestic species, or are private to continent-wide or individual sheep populations. Retained or introgressed wild gene variants in domestic populations have contributed to local adaptation, such as the variation in the HBB associated with plateau adaptation. We identified novel and previously reported targets of selection on morphological and agronomic traits such as stature, horn, tail configuration, and wool fineness. We explored the genetic basis of wool fineness and unveiled a novel mutation (chr25: T7,068,586C) in the 3′-UTR of IRF2BP2 as plausible causal variant for fleece fiber diameter. We reconstructed prehistorical migrations from the Near Eastern domestication center to South-and-Southeast Asia and found two main waves of migrations across the Eurasian Steppe and the Iranian Plateau in the Early and Late Bronze Ages. Our findings refine our understanding of genome variation as shaped by continental migrations, introgression, adaptation, and selection of sheep., This study was financially supported by grants from the National Natural Science Foundation of China (Nos. 32061133010, 31825024, 31661143014, and 31972527), the National Key Research and Development Program-Key Projects of International Innovation Cooperation between Governments (2017YFE0117900), the External Cooperation Program of Chinese Academy of Sciences (152111KYSB20150010), and the Second Tibetan Plateau Scientific Expedition and Research Program (STEP) (No. 2019QZKK0501), and the Special Funds of the State Key Laboratory of Sheep Genetic Improvement and Healthy Production (Nos. 2018CA001, and 2019CA009, 2020CA001).
- Published
- 2022
4. Genome Divergence and Dynamics in the Thin-Tailed Desert Sheep From Sudan.
- Author
-
Abied, Adam, Ahbara, Abulgasim M., Berihulay, Haile, Xu, Lingyang, Islam, Rabiul, El-Hag, Faisal M., Rekik, Mourad, Haile, Aynalem, Han, Jian-Lin, Ma, Yuehui, Zhao, Qianjun, and Mwacharo, Joram M.
- Subjects
SHEEP breeding ,GENOME-wide association studies ,SHEEP ,GENOMICS ,DESERTS ,GENETIC variation - Abstract
With climate change bound to affect food and feed production, emphasis will shift to resilient and adapted indigenous livestock to sustain animal production. However, indigenous livestock comprise several varieties, strains and ecotypes whose genomes are poorly characterized. Here, we investigated genomic variation in an African thin-tailed Desert Sheep sampled in Sudan, using 600K genotype data generated from 92 individuals representing five ecotypes. We included data from 18 fat-tailed and 45 thin-tailed sheep from China, to investigate shared ancestry and perform comparative genomic analysis. We observed a clear genomic differentiation between the African thin-tailed Desert Sheep and the Chinese thin-tailed and fat-tailed sheep, suggesting a broad genetic structure between the fat-tailed and thin-tailed sheep in general, and that at least two autosomal gene pools comprise the genome profile of the thin-tailed sheep. Further analysis detected two distinct genetic clusters in both the African thin-tailed Desert Sheep and the Chinese thin-tailed sheep, suggesting a fine-scale and complex genome architecture in thin-tailed sheep. Selection signature analysis suggested differences in adaptation, production, reproduction and morphology likely underly the fine-scale genetic structure in the African thin-tailed Desert Sheep. This may need to be considered in designing breeding programs and genome-wide association studies. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
5. Mitochondrial DNA variation reveals maternal origins and demographic dynamics of Ethiopian indigenous goats.
- Author
-
Tarekegn, Getinet Mekuriaw, Tesfaye, Kassahun, Mwai, Okeyo Ally, Djikeng, Appolinaire, Dessie, Tadelle, Birungi, Josephine, Osama, Sarah, Zergaw, Netsanet, Alemu, Alubel, Achieng, Gloria, Tutah, Jack, Mutai, Collins, Njuguna, Joyce, and Mwacharo, Joram M.
- Subjects
GOAT genetics ,MITOCHONDRIAL DNA ,BIOLOGICAL variation ,HAPLOTYPES ,ANIMALS - Abstract
Abstract: The Horn of Africa forms one of the two main historical entry points of domestics into the continent and Ethiopia is particularly important in this regard. Through the analysis of mitochondrial DNA (mtDNA)
d ‐loop region in 309 individuals from 13 populations, we reveal the maternal genetic variation and demographic dynamics of Ethiopian indigenous goats. A total of 174 variable sites that generated 231 haplotypes were observed. They defined two haplogroups that were present in all the 13 study populations. Reference haplotypes from the six globally defined goat mtDNA haplogroups show the two haplogroups present in Ethiopia to be A and G, the former being the most predominant. Although both haplogroups are characterized by an increase in effective population sizes (N e ) predating domestication, they also have experienced a decline inN e at different time periods, suggesting different demographic histories. We observed seven haplotypes, six were directly linked to the central haplotypes of the two haplogroups and one was central to haplogroup G. The seven haplotypes were common between Ethiopia, Kenya, Egypt, and Saudi Arabia populations, suggesting common maternal history and the introduction of goats into East Africa via Egypt and the Arabian Peninsula, respectively. While providing new mtDNA data from a historically important region, our results suggest extensive intermixing of goats mediated by human socio‐cultural and economic interactions. These have led to the coexistence of the two haplogroups in different geographic regions in Ethiopia resulting in a large caprine genetic diversity that can be exploited for genetic improvement. [ABSTRACT FROM AUTHOR]- Published
- 2018
- Full Text
- View/download PDF
6. Multi-locus genotyping reveals absence of genetic structure in field populations of the brown ear tick (Rhipicephalus appendiculatus) in Kenya.
- Author
-
Kanduma, Esther G., Mwacharo, Joram M., Mwaura, Stephen, Njuguna, Joyce N., Nzuki, Inosters, Kinyanjui, Peter W., Githaka, Naftaly, Heyne, Heloise, Hanotte, Olivier, Skilton, Robert A., and Bishop, Richard P.
- Abstract
Rhipicephalus appendiculatus is an important tick vector of several pathogens and parasitizes domestic and wild animals across eastern and southern Africa. However, its inherent genetic variation and population structure is poorly understood. To investigate whether mammalian host species, geographic separation and resulting reproductive isolation, or a combination of these, define the genetic structure of R. appendiculatus , we analyzed multi-locus genotype data from 392 individuals from 10 geographic locations in Kenya generated in an earlier study. These ticks were associated with three types of mammalian host situations; (1) cattle grazing systems, (2) cattle and wildlife co-grazing systems (3) wildlife grazing systems without livestock. We also analyzed data from 460 individuals from 10 populations maintained as closed laboratory stocks and 117 individuals from five other species in the genus Rhipicephalus. The pattern of genotypes observed indicated low levels of genetic differentiation between the ten field populations ( F ST = 0.014 ± 0.002) and a lack of genetic divergence corresponding to the degree of separation of the geographic sampling locations. There was also no clear association of particular tick genotypes with specific host species. This is consistent with tick dispersal over large geographic ranges and lack of host specificity. In contrast, the 10 laboratory populations ( F ST = 0.248 ± 0.015) and the five other species of Rhipicephalus ( F ST = 0.368 ± 0.032) were strongly differentiated into distinct genetic groups. Some laboratory bred populations diverged markedly from their field counterparts in spite of originally being sampled from the same geographic locations. Our results demonstrate a lack of defined population genetic differentiation in field populations of the generalist R. appendiculatus in Kenya, which may be a result of the frequent anthropogenic movement of livestock and mobility of its several wildlife hosts between different locations. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
7. Micro- and minisatellite-expressed sequence tag (EST) markers discriminate between populations of Rhipicephalus appendiculatus.
- Author
-
Kanduma, Esther G., Mwacharo, Joram M., Sunter, Jack D., Nzuki, Inosters, Mwaura, Stephen, Kinyanjui, Peter W., Kibe, Michael, Heyne, Heloise, Hanotte, Olivier, Skilton, Robert A., and Bishop, Richard P.
- Abstract
Abstract: Biological differences, including vector competence for the protozoan parasite Theileria parva have been reported among populations of Rhipicephalus appendiculatus (Acari: Ixodidae) from different geographic regions. However, the genetic diversity and population structure of this important tick vector remain unknown due to the absence of appropriate genetic markers. Here, we describe the development and evaluation of a panel of EST micro- and minisatellite markers to characterize the genetic diversity within and between populations of R. appendiculatus and other rhipicephaline species. Sixty-six micro- and minisatellite markers were identified through analysis of the R. appendiculatus Gene Index (RaGI) EST database and selected bacterial artificial chromosome (BAC) sequences. These were used to genotype 979 individual ticks from 10 field populations, 10 laboratory-bred stocks, and 5 additional Rhipicephalus species. Twenty-nine markers were polymorphic and therefore informative for genetic studies while 6 were monomorphic. Primers designed from the remaining 31 loci did not reliably generate amplicons. The 29 polymorphic markers discriminated populations of R. appendiculatus and also 4 other Rhipicephalus species, but not R. zambeziensis. The percentage Principal Component Analysis (PCA) implemented using Multiple Co-inertia Analysis (MCoA) clustered populations of R. appendiculatus into 2 groups. Individual markers however differed in their ability to generate the reference typology using the MCoA approach. This indicates that different panels of markers may be required for different applications. The 29 informative polymorphic micro- and minisatellite markers are the first available tools for the analysis of the phylogeography and population genetics of R. appendiculatus. [Copyright &y& Elsevier]
- Published
- 2012
- Full Text
- View/download PDF
8. The wild species genome ancestry of domestic chickens
- Author
-
Lawal, Raman Akinyanju, Martin, Simon H, Vanmechelen, Koen, Vereijken, Addie, Silva, Pradeepa, Al-Atiyat, Raed Mahmoud, Aljumaah, Riyadh Salah, Mwacharo, Joram M, Wu, Dong-Dong, Zhang, Ya-Ping, Hocking, Paul M, Smith, Jacqueline, Wragg, David, and Hanotte, Olivier
- Subjects
2. Zero hunger ,animal structures ,Genome ,Chicken domestication ,Evolution ,Speciation ,Animals, Wild ,15. Life on land ,Genetic Introgression ,Biological Evolution ,Genetic diversity ,Interspecies hybridisation ,Livestock species ,Chicken introgression ,Gallus species ,Animals ,Galliformes ,Divergence time ,Chickens ,Phylogeny - Abstract
BACKGROUND: Hybridisation and introgression play key roles in the evolutionary history of animal species. They are commonly observed within several orders in wild birds. The domestic chicken Gallus gallus domesticus is the most common livestock species. More than 65 billion chickens are raised annually to produce meat and 80 million metric tons of egg for global human consumption by the commercial sector. Unravelling the origin of its genetic diversity has major application for sustainable breeding improvement programmes. RESULTS: In this study, we report genome-wide analyses for signatures of introgression between indigenous domestic village chicken and the four wild Gallus species. We first assess the genome-wide phylogeny and divergence time across the genus Gallus. Genome-wide sequence divergence analysis supports a sister relationship between the Grey junglefowl G. sonneratii and Ceylon junglefowl G. lafayettii. Both species form a clade that is sister to the Red junglefowl G. gallus, with the Green junglefowl G. varius the most ancient lineage within the genus. We reveal extensive bidirectional introgression between the Grey junglefowl and the domestic chicken and to a much lesser extent with the Ceylon junglefowl. We identify a single case of Green junglefowl introgression. These introgressed regions include genes with biological functions related to development and immune system. CONCLUSIONS: Our study shows that while the Red junglefowl is the main ancestral species, introgressive hybridisation episodes have impacted the genome and contributed to the diversity of the domestic chicken, although likely at different levels across its geographic range.
9. The wild species genome ancestry of domestic chickens
- Author
-
Lawal, Raman Akinyanju, Martin, Simon H., Vanmechelen, Koen, Vereijken, Addie, Silva, Pradeepa, Al-Atiyat, Raed Mahmoud, Aljumaah, Riyadh Salah, Mwacharo, Joram M., Wu, Dong-Dong, Zhang, Ya-Ping, Hocking, Paul M., Smith, Jacqueline, Wragg, David, and Hanotte, Olivier
- Subjects
2. Zero hunger ,animal structures ,Chicken domestication ,Evolution ,Speciation ,15. Life on land ,Genetic diversity ,Livestock species ,Interspecies hybridisation ,Chicken introgression ,Gallus species ,Galliformes ,Divergence time ,Research Article - Abstract
Background: Hybridisation and introgression play key roles in the evolutionary history of animal species. They are commonly observed within several orders in wild birds. The domestic chicken Gallus gallus domesticus is the most common livestock species. More than 65 billion chickens are raised annually to produce meat and 80 million metric tons of egg for global human consumption by the commercial sector. Unravelling the origin of its genetic diversity has major application for sustainable breeding improvement programmes. Results: In this study, we report genome-wide analyses for signatures of introgression between indigenous domestic village chicken and the four wild Gallus species. We first assess the genome-wide phylogeny and divergence time across the genus Gallus. Genome-wide sequence divergence analysis supports a sister relationship between the Grey junglefowl G. sonneratii and Ceylon junglefowl G. lafayettii. Both species form a clade that is sister to the Red junglefowl G. gallus, with the Green junglefowl G. varius the most ancient lineage within the genus. We reveal extensive bidirectional introgression between the Grey junglefowl and the domestic chicken and to a much lesser extent with the Ceylon junglefowl. We identify a single case of Green junglefowl introgression. These introgressed regions include genes with biological functions related to development and immune system. Conclusions: Our study shows that while the Red junglefowl is the main ancestral species, introgressive hybridisation episodes have impacted the genome and contributed to the diversity of the domestic chicken, although likely at different levels across its geographic range.
10. Mitochondrial and nuclear multilocus phylogeny of Rhipicephalus ticks from Kenya.
- Author
-
Kanduma, Esther G., Bishop, Richard P., Githaka, Naftaly W., Skilton, Robert A., Heyne, Heloise, and Mwacharo, Joram M.
- Subjects
- *
RIBOSOMAL DNA , *RHIPICEPHALUS , *TICKS , *CYTOCHROME oxidase , *NUCLEAR DNA , *PHYLOGENY - Abstract
• COI divergence among seven rhipicephaline ticks found in Kenya ranges from 9–16%. • R. simus / R. praetextatus / R. muhsamae comprise a closely related tick species complex. • Ruma National Park tick species is genetically related to those of R. simus complex. • Molecular identification is only reliable when well curated reference sequences exist. The morphological diversity of African ticks of the genus Rhipicephalus and subgenus Boophilus have been studied in detail. However, their taxonomy remains poorly resolved with limited molecular studies performed to improve inter-species discrimination. Herein, ribosomal cytochrome c oxidase I (COI), 12S ribosomal DNA (12S rDNA) and nuclear ribosomal DNA internal transcriber spacer 2 (ITS2) were analyzed in Rhipicephalus tick populations in Kenya. While the morphological and molecular criteria separated R. e. evertsi, R. pulchellus and R. appendiculatus from other members of the genus, except the morphologically similar sibling species R. zambeziensis , this was not the case for other tick populations. COI sequences of Rhipicephalus ticks from Ruma National Park (RNP) in Southwestern Kenya, that were morphologically similar to R. praetextatus/R. simus , a formed distinct clade and barcode gap group. 12S rDNA haplotypes of this population were 99% identical to a GenBank accession of R. muhsamae which is thought to be endemic in West and Central Africa. However, the ITS2 locus indicated that the RNP samples were genetically closest to ticks identified morphologically as R. praetextatus. The COI and 12S rDNA haplotype sequences of R. praetextatus clustered closely with R. simus reference sequences though the two species occurred in distinct barcode gap groups. Our results suggest that the R. simus/R. praetextatus / R. muhsamae comprise a closely related tick species complex found across sub-Saharan Africa and includes the yet to be described RNP population. More studies on the biology, ecology and genomics of all life stages of tick species in the complex may clarify their taxonomic status. A continent-wide study that combines morphology, DNA marker sequencing and emerging methods, such as mass spectrometry and whole-genome resequencing may reveal the diversity and distribution of taxa within the genus Rhipicephalus in sub-Saharan Africa. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.