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Forty-four microsatellite DNA markers were developed for silver carp, and used to investigate polymorphisms of 41 wild silver carps and seven wild bighead carps collected from Jingzhou fragment of Yangtze River. In silver carp, 40 markers were polymorphic. A total of 297 alleles were detected at 40 polymorphic loci. The number of alleles per locus varied from two to 16 with an average of 7.4 and the expected heterozygosities of each locus ranged from 0.07 to 0.91 with an average of 0.69. All markers amplified both silver carp and bighead carp DNAs. [ABSTRACT FROM AUTHOR]

Abstract: Genetic linkage maps of guppy (Poecilia reticulata) were constructed using microsatellite DNA and AFLP markers and a pseudo-testcross mapping strategy. (AC)n microsatellite DNA markers were developed and used to genotype sixty-one full-sib progenies (F1) and their two parents. Of 293 microsatellite DNA markers, 101 segregated at 1:1 or 1:1:1:1 ratios. In addition, 336 AFLP markers segregated also in F1 progenies at 1:1 ratio, which were produced using 91 primer combinations. All these markers were mapped with two linkage maps produced, one each parent. Female map included 135 markers in 22 linkage groups, covering a total of 1267.7 cM in length. Male map included 172 markers in 20 linkage groups, covering a total of 1771.2 cM in length. [Copyright &y& Elsevier]