1. Kdm3b haploinsufficiency impairs the consolidation of cerebellum-dependent motor memory in mice.
- Author
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Kim, Yong Gyu, Bak, Myeong Seong, Kim, Ahbin, Kim, Yujin, Chae, Yun-Cheol, Kim, Ye Lee, Chun, Yang-Sook, An, Joon-Yong, Seo, Sang-Beom, Kim, Sang Jeong, and Lee, Yong-Seok
- Subjects
MOTOR learning ,CEREBELLAR cortex ,GENETIC regulation ,GRANULE cells ,HISTONE methylation ,MEMORY ,MICE - Abstract
Histone modifications are a key mechanism underlying the epigenetic regulation of gene expression, which is critically involved in the consolidation of multiple forms of memory. However, the roles of histone modifications in cerebellum-dependent motor learning and memory are not well understood. To test whether changes in histone methylation are involved in cerebellar learning, we used heterozygous Kdm3b knockout (Kdm3b
+/− ) mice, which show reduced lysine 9 on histone 3 (H3K9) demethylase activity. H3K9 di-methylation is significantly increased selectively in the granule cell layer of the cerebellum of Kdm3b+/− mice. In the cerebellum-dependent optokinetic response (OKR) learning, Kdm3b+/− mice show deficits in memory consolidation, whereas they are normal in basal oculomotor performance and OKR acquisition. In addition, RNA-seq analyses revealed that the expression levels of several plasticity-related genes were altered in the mutant cerebellum. Our study suggests that active regulation of histone methylation is critical for the consolidation of cerebellar motor memory. [ABSTRACT FROM AUTHOR]- Published
- 2021
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