1. Knockdown of T-cell intracellular antigens triggers cell proliferation, invasion and tumour growth.
- Author
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José M. Izquierdo, José Alcalde, Isabel Carrascoso, Raquel Reyes, and María Dolores Ludeña
- Subjects
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T cells , *ANTIGENS , *CELL proliferation -- Molecular aspects , *TUMOR growth , *LABORATORY mice , *XENOGRAFTS , *GENETIC transcription , *EPITHELIAL tumors - Abstract
TIA (T-cell intracellular antigen) proteins function as DNA/RNA trans-acting regulators to expand transcriptome and proteome diversity in mammals. In the present paper we report that the stable silencing of TIA1 and/or TIAR/TIAL1 (TIA1-related/like protein 1) expression in HeLa cells enhances cell proliferation, anchorage-dependent and -independent growth and invasion. HeLa cells lacking TIA1 and/or TIAR generate larger and faster-growing epithelial tumours with high rates of proliferation and angiogenesis in nude mice xenografts. Protein array analysis of a collection of human tumours shows that TIA1 and TIAR protein expression is down-regulated in a subset of epithelial tumours relative to normal tissues. Our results suggest a link between the epigenetic control exerted by TIA proteins and the transcriptional and post-transcriptional regulation of a subset of specific genes involved in tumour progression. Taken together, these results are consistent with a role for TIA proteins as growth/tumour-suppressor genes. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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