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Summary. Grapevine Pinot gris virus (GPGV) is a recently identified trichovirus infecting grapevines. Despite wide distribution, there is limited available information on epidemiology, transmission, and associated symptoms of grapevine leaf mottling and deformation. Occurrence and genetic diversity of GPGV variants were surveyed in an organically cultivated Hungarian vineyard that was planted between 1996 and 2014. Sequence analysis demonstrated the widespread presence and high variability of GPGV, and according to phylogenetic analyses, the Hungarian virus isolates were clas- sified into three groups. Most of the identified variants clustered with the representa- tive asymptomatic isolates, but all isolates from one grapevine cultivar grouped with representative isolates of clade B. Furthermore, one isolate clustered with representative isolates of clade C, and the identified clade C variant had previously undescribed poly- morphisms. [ABSTRACT FROM AUTHOR]

Blackleg (stem canker) of crucifers is a globally important disease caused by multiple genetic subclades of the fungi Plenodomuslingam (syn.: Leptosphaeria maculans) and Plenodomus biglobosus (syn.: Leptosphaeria biglobosa). In our study, we monitored the geographical distribution of these two pathogen species from rapeseed growing areas in Hungary. Multiplex PCR identified 48.7% of the isolates as Plenodomus biglobosus, which indicates the non-recent introduction of the pathogen into Hungary. In addition, multi-locus analysis revealed low genetic diversity within the species, as all isolates were clustered to the Plenodomuslingam 'brassicae' and Plenodomus biglobosus 'brassicae' subclades. The low genetic diversity of a population generally means reduced adaptation potential, which is essential information in breeding and in developing more effective management strategies. [ABSTRACT FROM AUTHOR]