9 results on '"Farhadi, Elham"'
Search Results
2. Polygenic Risk Scores have high diagnostic capacity in ankylosing spondylitis.
- Author
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Zhixiu Li, Xin Wu, Leo, Paul J., De Guzman, Erika, Akkoc, Nurullah, Breban, Maxime, Macfarlane, Gary J., Mahmoudi, Mahdi, Ortega, Helena Marzo, Anderson, Lisa K., Wheeler, Lawrie, Chung- Tei Chou, Harrison, Andrew A., Stebbings, Simon, Jones, Gareth T., So- Young Bang, Geng Wang, Jamshidi, Ahmadreza, Farhadi, Elham, and Jing Song Li Lin
- Subjects
BACKACHE diagnosis ,CHRONIC pain ,SACROILIAC joint ,C-reactive protein ,RESEARCH ,GENETICS ,HLA-B27 antigen ,RESEARCH evaluation ,ANKYLOSING spondylitis ,RESEARCH methodology ,MAGNETIC resonance imaging ,CASE-control method ,BACKACHE ,MEDICAL cooperation ,EVALUATION research ,COMPARATIVE studies ,RESEARCH funding - Abstract
Objective: We sought to test the hypothesis that Polygenic Risk Scores (PRSs) have strong capacity to discriminate cases of ankylosing spondylitis (AS) from healthy controls and individuals in the community with chronic back pain.Methods: PRSs were developed and validated in individuals of European and East Asian ethnicity, using data from genome-wide association studies in 15 585 AS cases and 20 452 controls. The discriminatory values of PRSs in these populations were compared with other widely used diagnostic tests, including C-reactive protein (CRP), HLA-B27 and sacroiliac MRI.Results: In people of European descent, PRS had high discriminatory capacity with area under the curve (AUC) in receiver operator characteristic analysis of 0.924. This was significantly better than for HLA-B27 testing alone (AUC=0.869), MRI (AUC=0.885) or C-reactive protein (AUC=0.700). PRS developed and validated in individuals of East Asian descent performed similarly (AUC=0.948). Assuming a prior probability of AS of 10% such as in patients with chronic back pain under 45 years of age, compared with HLA-B27 testing alone, PRS provides higher positive values for 35% of patients and negative predictive values for 67.5% of patients. For PRS, in people of European descent, the maximum positive predictive value was 78.2% and negative predictive value was 100%, whereas for HLA-B27, these values were 51.9% and 97.9%, respectively.Conclusions: PRS have higher discriminatory capacity for AS than CRP, sacroiliac MRI or HLA-B27 status alone. For optimal performance, PRS should be developed for use in the specific ethnic groups to which they are to be applied. [ABSTRACT FROM AUTHOR]- Published
- 2021
- Full Text
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3. Genome-wide association study in Turkish and Iranian populations identify rare familial Mediterranean fever gene (MEFV) polymorphisms associated with ankylosing spondylitis.
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Li, Zhixiu, Akar, Servet, Yarkan, Handan, Lee, Sau Kuen, Çetin, Pınar, Can, Gerçek, Kenar, Gökce, Çapa, Fernur, Pamuk, Omer Nuri, Pehlivan, Yavuz, Cremin, Katie, De Guzman, Erika, Harris, Jessica, Wheeler, Lawrie, Jamshidi, Ahmadreza, Vojdanian, Mahdi, Farhadi, Elham, Ahmadzadeh, Nooshin, Yüce, Zeynep, and Dalkılıç, Ediz
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ANKYLOSING spondylitis ,FAMILIAL Mediterranean fever ,ARTHRITIS ,HUMAN genome ,GENETIC polymorphisms ,INTERLEUKINS - Abstract
Ankylosing spondylitis (AS) is a highly heritable immune-mediated arthritis common in Turkish and Iranian populations. Familial Mediterranean Fever (FMF) is an autosomal recessive autoinflammatory disease most common in people of Mediterranean origin. MEFV, an FMF-associated gene, is also a candidate gene for AS. We aimed to identify AS susceptibility loci and also examine the association between MEFV and AS in Turkish and Iranian cohorts. We performed genome-wide association studies in 1001 Turkish AS patients and 1011 Turkish controls, and 479 Iranian AS patients and 830 Iranian controls. Serum IL-1β, IL-17 and IL-23 cytokine levels were quantified in Turkish samples. An association of major effect was observed with a novel rare coding variant in MEFV in the Turkish cohort (rs61752717, M694V, OR = 5.3, P = 7.63×10
−12 ), Iranian cohort (OR = 2.9, P = 0.042), and combined dataset (OR = 5.1, P = 1.65×10−13 ). 99.6% of Turkish AS cases, and 96% of those carrying MEFV rs61752717 variants, did not have FMF. In Turkish subjects, the association of rs61752717 was particularly strong in HLA-B27-negative cases (OR = 7.8, P = 8.93×10−15 ), but also positive in HLA-B27-positive cases (OR = 4.3, P = 7.69×10−8 ). Serum IL-1β, IL-17 and IL-23 levels were higher in AS cases than controls. Among AS cases, serum IL-1β and IL-23 levels were increased in MEFV 694V carriers compared with non-carriers. Our data suggest that FMF and AS have overlapping aetiopathogenic mechanisms. Functionally important MEFV mutations, such as M694V, lead to dysregulated inflammasome function and excessive IL-1β function. As IL-1 inhibition is effective in FMF, AS cases carrying FMF-associated MEFV variants may benefit from such therapy. [ABSTRACT FROM AUTHOR]- Published
- 2019
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4. Association of T Helper 1 Cytokine Gene Single Nucleotide Polymorphisms with Ulcerative Colitis and Crohn's Disease.
- Author
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Ebrahimi Daryani, Nasser, Sadr, Maryam, Soltani, Samaneh, Saghazadeh, Amene, Moossavi, Shirin, Shahkarami, Sepideh, Farhadi, Elham, and Rezaei, Nima
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CYTOKINE genetics ,INTERLEUKIN-2 ,SINGLE nucleotide polymorphisms ,ULCERATIVE colitis ,CROHN'S disease - Abstract
Background: Inflammatory bowel disease (IBD) mostly comprised of Crohn's disease (CD) and ulcerative colitis (UC) is a condition arising from the combined effects of genetic, environmental, and immunological factors. IBD is associated with inflammation and altered cytokine profile. Objective: This study was aimed at assessing the association between T helper type 1 (Th1) cytokine polymorphisms (interferon gamma [IFN-γ] +874 A/T, interleukin-12 [IL-12] –1188 A/C, IL-2 –330 G/T, IL-2 +166 G/T) and susceptibility to and clinical features of IBD. Methods: The study population was composed of 75 IBD patients (40 CD patients and 35 UC patients) and 140 healthy controls. Genotyping was performed using polymerase chain reaction with sequence-specific primers. Results: The A allele of IFN-γ +874 polymorphism was overrepresented in the whole population of patients with IBD (OR 1.63; 95% CI 1.08–2.47; p = 0.020) and as well in the subpopulation of patients with CD (OR 2.14; 95% CI 1.26–3.63; p = 0.004), but not in UC. Multiple pairwise comparisons indicated that genotypes of single nucleotide polymorphisms (SNPs) within the IL-2 and IFN-γ genes are correlated with IBD, CD, and UC, while neither allele nor genotype frequency of th1 IL-12 –1188 polymorphism was associated with IBD, CD, or UC. Haplotype analysis also revealed that the presence of IL-2 –330/+166 TG haplotype versus the remaining haplotypes (GG, TT, and GT) is a protective factor against IBD (OR 0.62; p = 0.046). Conclusions: The present study reports (for the first time) significant associations between SNPs within the IFN-γ and IL-2 genes and IBD. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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5. Association of Transforming Growth Factor-Beta Gene Polymorphisms in Recurrent Aphthous Stomatitis.
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Yousefi, Hila, Najafi, Shamsolmoulouk, Mohammadzadeh, Mahsa, Bidoki, Alireza Zare, Farhadi, Elham, and Rezaei, Nima
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STOMATITIS treatment ,GENOTYPES ,GENE frequency ,POLYMERASE chain reaction ,HAPLOTYPES - Abstract
This study has been conducted to evaluate the allele, genotype and haplotype frequencies of the polymorphic gene coding TGF-β in recurrent aphthous stomatitis (RAS). TGF-β gene typing was done by polymerase chain reaction with sequence-specific primers (PCR-SSP) assay. Allele frequencies were estimated by direct gene counting. C allele at codon 25 was significantly increased, while G allele at this position was significantly decreased in patients compared to the controls. A significantly higher frequency of CG genotype at codon 25 was found in control group. CC genotype and TT genotype at codon 10 of the gene was significantly decreased, while CT genotype at the same position was significantly increased in patients, indicating that CT heterozygosity at codon 10 TGF-β is associated with greater risk of RAS. CG and TG haplotypes were significantly decreased while CC and TC haplotypes were significantly increased in patients compared with controls. This study indicates the TGF-β single nucleotide polymorphisms could play a role in RAS pathogenesis. Thereby certain SNPs of TGF-β gene have an association with RAS pathogenesis. © 2017 Tehran University of Medical Sciences. All rights reserved. [ABSTRACT FROM AUTHOR]
- Published
- 2017
6. Interleukin-4 and Interleukin-10 Gene Polymorphisms in Patients with Inflammatory Bowel Disease.
- Author
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Ebrahimi Daryani, Nasser, Saghazadeh, Amene, Moossavi, Shirin, Sadr, Maryam, Shahkarami, Sepideh, Soltani, Samaneh, Farhadi, Elham, and Rezaei, Nima
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INFLAMMATORY bowel diseases ,INTERLEUKIN-4 ,INTERLEUKIN-10 ,GENETIC polymorphisms ,PROTEIN expression ,PATIENTS - Abstract
Background: Changes in cytokine expression have been frequently found in patients with inflammatory bowel disease (IBD). Cytokine values outside the normal range may be somewhat related to common polymorphisms within cytokine genes. Objective: The present study was designed to investigate the possible association between polymorphisms within Interleukin IL-4 and IL-10 genes and susceptibility to and clinical features of IBD. Methods: The study population was composed of 140 healthy controls and 75 patients with IBD (40 patients with Crohn’s disease (CD) and 35 patients with ulcerative colitis (UC)). Genotyping was performed using polymerase chain reaction with sequence-specific primers. Results: Higher frequencies for the C allele of IL-4–590 polymorphism (P < 0.0001; odds ratio [OR], 5.68; 95% confidence interval [95% CI], 3.28–9.83) and for the T allele of IL-4–1098 polymorphism (P = 0.016; OR, 1.83; 95% CI, 1.11–3.02) were observed in the whole group of IBD patients. The IL-4–590 C allele was also significantly overrepresented when IBD patients were subdivided into CD and UC (P < 0.0001; OR, 5.2–6.28). While the IL-4–1098 T allele was present at higher frequencies in patients with UC (P = 0.05; OR, 1.95), but not in CD (P = 0.09). Multiple pairwise comparisons indicated that genotypes of all polymorphisms investigated within IL-4 gene are correlated with IBD, CD, and UC. Haplotype analysis showed that the IL-4–1098/-590 TC haplotype might predispose individuals to IBD, CD, and UC whereas the IL-4–1098/-590 TT and GC haplotypes have a protective effect. On the contrary, neither allele nor genotype frequencies of IL-10 polymorphisms (IL-10–1082 A > G, IL-10–592 A > C, and IL-10–819 T > C) were associated with IBD, CD, or UC. Conclusions: The present study suggests that IL-4 polymorphisms might play a role in susceptibility to IBD and its major subtypes in the Iranian population. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
7. Analysis of Killer Cell Immunoglobulin-like Receptor Genes and Their HLA Ligands in Iranian Patients with Ankylosing Spondylitis.
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Mahmoudi, Mahdi, Jamshidi, Ahmad Reza, Karami, Jafar, Mohseni, Alireza, Amirzargar, Ali Akbar, Farhadi, Elham, Ahmadzadeh, Nooshin, Nicknam, Mohammad Hossein, and Mahmoudi, Mehdi
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ANKYLOSING spondylitis ,SPONDYLOARTHROPATHIES ,KILLER cells ,CELL-mediated cytotoxicity ,HLA histocompatibility antigens ,HLA-B27 antigen ,CELL receptors ,DISEASE susceptibility ,GENETICS ,LIGANDS (Biochemistry) ,PHENOTYPES ,CASE-control method ,DIAGNOSIS - Abstract
Ankylosing Spondylitis (AS) is a chronic rheumatic disease which mainly involves the axial skeleton. It seems that non-HLA genes, as well as HLA-B27 gene, are linked to the etiology of the disease. Recently, it has been documented that KIRs and their HLA ligands are contributed to the Ankylosing Spondylitis. The aim of this study was to evaluate the KIR genes and their HLA ligands in Iranian AS patients and healthy individuals. The present study includes 200 AS patient samples and 200 healthy control samples. KIR genotyping was performed using the polymerase chain reaction sequence-specific primer (PCR-SSP) method to type the presence or absence of the 16 KIR genes, 6 known specific HLA class I ligands and also, two pseudogenes. Two KIR genes (KIR-2DL3 and KIR2DL5), and among the HLA ligands, two HLA ligands (HLA-C2Lys80 and HLA-B27) genes were significantly different between case and control groups. In addition, we found some interesting KIR/HLA compound genotypes, which were associated with AS susceptibility. Our results suggest that the AS patients present more activating and less inhibitory KIR genes with combination of their HLA ligands than healthy controls. Once the balance of signal transduction between activating and inhibitory receptors is disturbed, the ability of NK cells to identify and lyse the targets in immune responses will be compromised. Accordingly, imbalance of activating and inhibitory KIR genes by up-regulating the activation and losing the inhibition of KIRs signaling or combination of both might be one of the important factors which underlying the pathogenesis of AS. [ABSTRACT FROM AUTHOR]
- Published
- 2016
8. Interleukin-2 and Interferon-Gamma Single Nucleotide Polymorphisms in Iranian Patients with Chronic Heart Failure.
- Author
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Mahmoudi, Mohammad Jafar, Harsini, Sara, Farhadi, Elham, Hedayat, Mona, Taghvaei, Mohammad, Mahmoudi, Maryam, Sadr, Maryam, Esfahanian, Nilufar, Nematipour, Ebrahim, Nourijelyani, Keramat, Amirzargar, Ali Akbar, and Rezaei, Nima
- Subjects
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CORONARY heart disease complications , *ALLELES , *DISEASE susceptibility , *GENETIC polymorphisms , *HEART failure , *INTERFERONS , *INTERLEUKIN-2 , *POLYMERASE chain reaction , *DISEASE prevalence , *CASE-control method , *HAPLOTYPES , *SEQUENCE analysis , *GENOTYPES , *GENETICS - Abstract
Background: Inflammatory cytokines have been known to be associated with Chronic Heart Failure (CHF). Given the importance of cytokines in the context of the failing heart, the prevalence of Interleukin-2 (IL-2) and Interferon-gamma (IFN-γ) polymorphisms was studied in patients with CHF due to ischemic heart disease in a case-control study. Methods: Fifty-six Iranian patients with CHF were enrolled in this study as the case group and compared with 139 healthy subjects, using polymerase chain reaction with sequence-specific primers method, so as to determine the frequency of alleles, genotypes and haplotypes of IFN-γ (+874 A/T) and IL-2 (-330 G/T, +166 G/T) SNPs. Results: The GG genotype at IL-2 -330 in patients with CHF was significantly over-represented in comparison with the control group (p=0.013). Such a positive genotypic association was also observed for IL-2 +166/TT (p=0.022). Meanwhile, the GT genotype frequency at IL-2 -330/GT in the patient group was significantly lower than the one in healthy controls (p=0.049). No significant association was detected between the IFN-γ gene polymorphisms and individuals' susceptibility to CHF. Conclusion: Certain genotypes in IL-2 gene were overrepresented in patients with CHF, which could render individuals more vulnerable to this disease. [ABSTRACT FROM AUTHOR]
- Published
- 2018
9. Tumor Necrosis Factor-Alpha and Interleukin-6 Gene Polymorphisms in Iranian Patients with Ischemic Heart Failure.
- Author
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Hedayat, Mona, Mahmoudi, Mohammad Jafar, Taghvaei, Mohammad, Nematipour, Ebrahim, Farhadi, Elham, Esfahanian, Nilufar, Mahmoudi, Maryam, Sadr, Maryam, Nourijelyani, Keramat, Amirzargar, Ali Akbar, and Rezaei, Nima
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CORONARY disease , *ALLELES , *GENETIC polymorphisms , *INTERLEUKINS , *POLYMERASE chain reaction , *TUMOR necrosis factors , *HAPLOTYPES , *GENOTYPES , *GENETICS - Abstract
Background: Proinflammatory cytokines have been known to be elevated in patients with Chronic Heart Failure (CHF). Given the importance of proinflammatory cytokines in the context of the failing heart, the prevalence of Tumor Necrosis Factor-α (TNF-α), Interleukin (IL)-6 polymorphisms in patients with CHF was studied due to ischemic heart disease. Methods: Forty three patients with ischemic heart failure were enrolled in this study and compared with 140 healthy individuals. The allele and genotype frequency of four Single Nucleotide Polymorphisms (SNPs) within the IL-6 (-174, nt565) and TNF-α (-308, -238) genes were determined, using Polymerase Chain Reaction with Sequence-Specific Primers (PCR-SSP) assay. Results: The frequency of the TNF-α (-238) A/A genotype was significantly higher in patients comparing to controls (p=0.043), while TNF-α G/A genotype at the same position decreased significantly, in comparison with controls (p=0.018). The most frequent haplotype for TNF-α was A/A in the patient group in comparison with controls (p=0.003). There was no significant difference in allele and genotype frequencies of IL-6 at positions -174 and nt565, and TNF-α at position -308. Conclusion: Certain alleles, genotypes, and haplotypes in TNF-α, but not IL-6, gene were overrepresented in patients with ischemic heart failure, which may, in turn, predispose individuals to this disease. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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