20 results on '"Liu, Jianjun"'
Search Results
2. Genetic Diversity and Structure of Sinopodophyllum hexandrum (Royle) Ying in the Qinling Mountains, China.
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Liu, Wei, Yin, Dongxue, Liu, Jianjun, and Li, Na
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PLANT diversity , *MEDICINAL plants , *PLANT conservation , *IN situ hybridization - Abstract
Sinopodophyllum hexandrum is an important medicinal plant whose genetic diversity must be conserved because it is endangered. The Qinling Mts. are a S. hexandrum distribution area that has unique environmental features that highly affect the evolution of the species. To provide the reference data for evolutionary and conservation studies, the genetic diversity and population structure of S. hexandrum in its overall natural distribution areas in the Qinling Mts. were investigated through inter-simple sequence repeats analysis of 32 natural populations. The 11 selected primers generated a total of 135 polymorphic bands. S. hexandrum genetic diversity was low within populations (average He = 0.0621), but higher at the species level (He = 0.1434). Clear structure and high genetic differentiation among populations were detected by using the unweighted pair group method for arithmetic averages, principle coordinate analysis and Bayesian clustering. The clustering approaches supported a division of the 32 populations into three major groups, for which analysis of molecular variance confirmed significant variation (63.27%) among populations. The genetic differentiation may have been attributed to the limited gene flow (Nm = 0.3587) in the species. Isolation by distance among populations was determined by comparing genetic distance versus geographic distance by using the Mantel test. Result was insignificant (r = 0.212, P = 0.287) at 0.05, showing that their spatial pattern and geographic locations are not correlated. Given the low within-population genetic diversity, high differentiation among populations and the increasing anthropogenic pressure on the species, in situ conservation measures were recommended to preserve S. hexandrum in Qinling Mts., and other populations must be sampled to retain as much genetic diversity of the species to achieve ex situ preservation as a supplement to in situ conservation. [ABSTRACT FROM AUTHOR]
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- 2014
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3. Swedish Population Substructure Revealed by Genome-Wide Single Nucleotide Polymorphism Data.
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Salmela, Elina, Lappalainen, Tuuli, Liu, Jianjun, Sistonen, Pertti, Andersen, Peter M., Schreiber, Stefan, Savontaus, Marja-Liisa, Czene, Kamila, Lahermo, Päivi, Hall, Per, and Kere, Juha
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SCANDINAVIANS , *POLYMORPHISM (Zoology) , *FINNS , *GENETICS , *STYE - Abstract
The use of genome-wide single nucleotide polymorphism (SNP) data has recently proven useful in the study of human population structure. We have studied the internal genetic structure of the Swedish population using more than 350,000 SNPs from 1525 Swedes from all over the country genotyped on the Illumina HumanHap550 array. We have also compared them to 3212 worldwide reference samples, including Finns, northern Germans, British and Russians, based on the more than 29,000 SNPs that overlap between the Illumina and Affymetrix 250K Sty arrays. The Swedes - especially southern Swedes - were genetically close to the Germans and British, while their genetic distance to Finns was substantially longer. The overall structure within Sweden appeared clinal, and the substructure in the southern and middle parts was subtle. In contrast, the northern part of Sweden, Norrland, exhibited pronounced genetic differences both within the area and relative to the rest of the country. These distinctive genetic features of Norrland probably result mainly from isolation by distance and genetic drift caused by low population density. The internal structure within Sweden (FST = 0.0005 between provinces) was stronger than that in many Central European populations, although smaller than what has been observed for instance in Finland; importantly, it is of the magnitude that may hamper association studies with a moderate number of markers if cases and controls are not properly matched geographically. Overall, our results underline the potential of genome-wide data in analyzing substructure in populations that might otherwise appear relatively homogeneous, such as the Swedes. [ABSTRACT FROM AUTHOR]
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- 2011
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4. Enhanced itaconic acid production in Yarrowia lipolytica via heterologous expression of a mitochondrial transporter MTT.
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Zhao, Chen, Cui, Zhiyong, Zhao, Xiangying, Zhang, Jiaxiang, Zhang, Lihe, Tian, Yanjun, Qi, Qingsheng, and Liu, Jianjun
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ITACONIC acid , *ACONITATE hydratase , *DECARBOXYLASES , *GENETICS , *BIOREACTORS - Abstract
Itaconic acid, a promising platform chemical, has been applied in many fields of industrial production. As a potential candidate for itaconic acid production, Yarrowia lipolytica possesses several innate abilities such as the tolerance of low-pH and high-shear stress, fast growth rate, cultivation flexibility, and easy for genetic manipulation. Here, Y. lipolytica Po1f which was tested to show high tolerance to itaconic acid could accumulate itaconic acid (0.363 g/L) by expressing the Aspergillus terreus cis-aconitic acid decarboxylase (CAD). Then, we tried to improve the supply and transport of the immediate precursor cis-aconitic acid by overexpressing a series of genes; these results indicate that overexpression of mitochondrial cis-aconitate transporter MTT is beneficial to the itaconic acid biosynthesis in Y. lipolytica. Further culture optimization enabled 22.03 g/L of itaconic acid to be produced in bioreactors, about 60-fold improvement over the initial titer, which is the highest itaconic acid production achieved at low pH by yeast reported worldwide, to data. This study demonstrates the great potential of Y. lipolytica as an industrial platform for itaconic acid production. [ABSTRACT FROM AUTHOR]
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- 2019
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5. Estimation of kinship coefficient in structured and admixed populations using sparse sequencing data.
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Dou, Jinzhuang, Sun, Baoluo, Sim, Xueling, Hughes, Jason D., Reilly, Dermot F., Tai, E. Shyong, Liu, Jianjun, and Wang, Chaolong
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GENE frequency , *NUCLEOTIDE sequencing , *HERITABILITY , *POPULATION genetics , *GENOMICS - Abstract
Knowledge of biological relatedness between samples is important for many genetic studies. In large-scale human genetic association studies, the estimated kinship is used to remove cryptic relatedness, control for family structure, and estimate trait heritability. However, estimation of kinship is challenging for sparse sequencing data, such as those from off-target regions in target sequencing studies, where genotypes are largely uncertain or missing. Existing methods often assume accurate genotypes at a large number of markers across the genome. We show that these methods, without accounting for the genotype uncertainty in sparse sequencing data, can yield a strong downward bias in kinship estimation. We develop a computationally efficient method called SEEKIN to estimate kinship for both homogeneous samples and heterogeneous samples with population structure and admixture. Our method models genotype uncertainty and leverages linkage disequilibrium through imputation. We test SEEKIN on a whole exome sequencing dataset (WES) of Singapore Chinese and Malays, which involves substantial population structure and admixture. We show that SEEKIN can accurately estimate kinship coefficient and classify genetic relatedness using off-target sequencing data down sampled to ~0.15X depth. In application to the full WES dataset without down sampling, SEEKIN also outperforms existing methods by properly analyzing shallow off-target data (~0.75X). Using both simulated and real phenotypes, we further illustrate how our method improves estimation of trait heritability for WES studies. [ABSTRACT FROM AUTHOR]
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- 2017
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6. Interaction Between Peroxisome Proliferator Activated Receptor δ and Epithelial Membrane Protein 2 Polymorphisms Influences HDL-C Levels in the Chinese Population.
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Ke, Tingjing, Dorajoo, Rajkumar, Han, Yi, Khor, Chiea‐Chuen, Dam, Rob M., Yuan, Jian‐Min, Koh, Woon‐Puay, Liu, Jianjun, Teo, Yik Ying, Goh, Daniel Y.T., Tai, E Shyong, Wong, Tien Yin, Cheng, Ching‐Yu, Friedlander, Yechiel, and Heng, Chew‐Kiat
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PEROXISOME proliferator-activated receptors , *EPITHELIAL cells , *GENETIC polymorphisms , *SINGLE nucleotide polymorphisms , *REGRESSION analysis - Abstract
Peroxisome proliferator activated receptors (PPARs) are transcription factors involved in the regulation of key metabolic pathways. Numerous in vivo and in vitro studies have established their important roles in lipid metabolism. A few SNPs in PPAR genes have been reported to be associated with lipid levels. In this study, we aimed to investigate the interactive effects between single nucleotide polymorphisms (SNPs) in three PPAR isoforms α/δ/γ and other genetic variants across the genome on plasma high-density lipoprotein-cholesterol (HDL-C) levels. Study subjects ( N = 2003) were genotyped using Illumina HumanOmniZhongHua-8 Beadchip. Fifty-three tag SNPs ± 100 kb of PPAR α, δ, and γ ( r2 < 0.2) were selected. The effect of interactions between PPAR SNPs and those across the genome on HDL-C was tested using linear regression models. One statistically significant interaction influencing HDL-C was detected between PPARδ SNP rs2267668 and epithelial membrane protein 2 ( EMP2) downstream SNP rs7191411 ( N = 1993, β = 0.74, adjusted P = 0.022). This interaction was successfully replicated in the meta-analysis of two additional Chinese cohorts ( N = 3948, P = 0.01). The present study showed a novel SNP × SNP interaction between rs2267668 in PPARδ and rs7191411 in EMP2 that has significant impact on circulating HDL-C levels in the Singaporean Chinese population. [ABSTRACT FROM AUTHOR]
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- 2016
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7. Phylogeography of Libanotis buchtormensis (Umbelliferae) in Disjunct Populations along the Deserts in Northwest China.
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Wang, Ping, Zhang, Xianzhi, Tang, Nan, Liu, Jianjun, Xu, Langran, and Wang, Kai
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UMBELLIFERAE , *PHYLOGEOGRAPHY , *PLANT diversity , *GENETIC speciation , *CHLOROPLASTS - Abstract
In Northwest China, aridification and desert expansion play significant roles in promoting desert plant diversification and speciation. However, to date, little is known about the effects of the desert barrier on the population structure of montane, non-desert species in the area. In this study, we sequenced chloroplast DNA regions (trnL–trnF and trnS–trnG) and a nuclear gene (rpb2) to investigate the population differentiation and phylogeographical history of Libanotis buchtormensis, a perennial montane species possessing a disjunct distribution at the periphery of the central desert. In total, 23 chloroplast haplotypes and 24 nuclear haplotypes were recovered from the 21 natural populations and six hebarium specimens. Phylogenetic analysis based on the combined plastid and nuclear dataset revealed two distinct lineages of L. buchtormensis, which inhabit the disjunct areas on both sides of the desert zone. The molecular dating analysis indicated that the divergence between the southeastern and the northwestern populations occurred in the middle Pleistocene, concomitantly with the desert expansion. The geographical vicariance likely contributed to the present disjunct distribution of L. buchtormensis across the deserts in Northwest China. Populations in the southeastern region may have migrated from the northwestern region, and seem to be a peripheral distribution of L. buchtormensis. [ABSTRACT FROM AUTHOR]
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- 2016
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8. Poly(ADP-Ribose) Glycohydrolase (PARG) Silencing Suppresses Benzo(a)pyrene Induced Cell Transformation.
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Li, Xuan, Li, Xiyi, Zhu, Zhiliang, Huang, Peiwu, Zhuang, Zhixiong, Liu, Jianjun, Gao, Wei, Liu, Yinpin, and Huang, Haiyan
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POLY ADP ribose , *HYDROLASES , *GENE silencing , *BENZOPYRENE , *CELL transformation , *POLLUTANTS - Abstract
Benzo(a)pyrene (BaP) is a ubiquitously distributed environmental pollutant and known carcinogen, which can induce malignant transformation in rodent and human cells. Poly(ADP-ribose) glycohydrolase (PARG), the primary enzyme that catalyzes the degradation of poly(ADP-ribose) (PAR), has been known to play an important role in regulating DNA damage repair and maintaining genomic stability. Although PARG has been shown to be a downstream effector of BaP, the role of PARG in BaP induced carcinogenesis remains unclear. In this study, we used the PARG-deficient human bronchial epithelial cell line (shPARG) as a model to examine how PARG contributed to the carcinogenesis induced by chronic BaP exposure under various concentrations (0, 10, 20 and 40 μM). Our results showed that PARG silencing dramatically reduced DNA damages, chromosome abnormalities, and micronuclei formations in the PARG-deficient human bronchial epithelial cells compared to the control cells (16HBE cells). Meanwhile, the wound healing assay showed that PARG silencing significantly inhibited BaP-induced cell migration. Furthermore, silencing of PARG significantly reduced the volume and weight of tumors in Balb/c nude mice injected with BaP induced transformed human bronchial epithelial cells. This was the first study that reported evidences to support an oncogenic role of PARG in BaP induced carcinogenesis, which provided a new perspective for our understanding in BaP exposure induced cancer. [ABSTRACT FROM AUTHOR]
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- 2016
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9. MicroRNA Related Polymorphisms and Breast Cancer Risk.
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Khan, Sofia, Greco, Dario, Michailidou, Kyriaki, Milne, Roger L., Muranen, Taru A., Heikkinen, Tuomas, Aaltonen, Kirsimari, Dennis, Joe, Bolla, Manjeet K., Liu, Jianjun, Hall, Per, Irwanto, Astrid, Humphreys, Keith, Li, Jingmei, Czene, Kamila, Chang-Claude, Jenny, Hein, Rebecca, Rudolph, Anja, Seibold, Petra, and Flesch-Janys, Dieter
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BREAST cancer risk factors , *MICRORNA , *GENETIC polymorphisms , *BINDING sites , *GENETIC regulation - Abstract
Genetic variations, such as single nucleotide polymorphisms (SNPs) in microRNAs (miRNA) or in the miRNA binding sites may affect the miRNA dependent gene expression regulation, which has been implicated in various cancers, including breast cancer, and may alter individual susceptibility to cancer. We investigated associations between miRNA related SNPs and breast cancer risk. First we evaluated 2,196 SNPs in a case-control study combining nine genome wide association studies (GWAS). Second, we further investigated 42 SNPs with suggestive evidence for association using 41,785 cases and 41,880 controls from 41 studies included in the Breast Cancer Association Consortium (BCAC). Combining the GWAS and BCAC data within a meta-analysis, we estimated main effects on breast cancer risk as well as risks for estrogen receptor (ER) and age defined subgroups. Five miRNA binding site SNPs associated significantly with breast cancer risk: rs1045494 (odds ratio (OR) 0.92; 95% confidence interval (CI): 0.88–0.96), rs1052532 (OR 0.97; 95% CI: 0.95–0.99), rs10719 (OR 0.97; 95% CI: 0.94–0.99), rs4687554 (OR 0.97; 95% CI: 0.95–0.99, and rs3134615 (OR 1.03; 95% CI: 1.01–1.05) located in the 3′ UTR of CASP8, HDDC3, DROSHA, MUSTN1, and MYCL1, respectively. DROSHA belongs to miRNA machinery genes and has a central role in initial miRNA processing. The remaining genes are involved in different molecular functions, including apoptosis and gene expression regulation. Further studies are warranted to elucidate whether the miRNA binding site SNPs are the causative variants for the observed risk effects. [ABSTRACT FROM AUTHOR]
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- 2014
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10. Analysis of POFUT1 Gene Mutation in a Chinese Family with Dowling-Degos Disease.
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Chen, Mingfei, Li, Yi, Liu, Hong, Fu, Xi'an, Yu, Yiongxiang, Yu, Gongqi, Wang, Chuan, Bao, Fangfang, Liany, Herty, Wang, Zhenzhen, Shi, Zhongxiang, Zhang, Dizhan, Zhou, Guizhi, Liu, Jianjun, and Zhang, Furen
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GENETIC mutation , *SKIN disease genetics , *CHINESE people , *GENETIC disorders , *HUMAN skin color , *NUCLEOTIDE sequence , *DISEASES - Abstract
Dowling-Degos disease (DDD) is an autosomal dominant genodermatosis characterized by reticular pigmented anomaly mainly affecting flexures. Though KRT5 has been identified to be the causal gene of DDD, the heterogeneity of this disease was displayed: for example, POFUT1 and POGLUT1 were recently identified and confirmed to be additional pathogenic genes of DDD. To identify other DDD causative genes, we performed genome-wide linkage and exome sequencing analyses in a multiplex Chinese DDD family, in which the KRT5 mutation was absent. Only a novel 1-bp deletion (c.246+5delG) in POFUT1 was found. No other novel mutation or this deletion was detected in POFUT1 in a second DDD family and a sporadic DDD case by Sanger Sequencing. The result shows the genetic-heterogeneity and complexity of DDD and will contribute to the further understanding of DDD genotype/phenotype correlations and to the pathogenesis of this disease. [ABSTRACT FROM AUTHOR]
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- 2014
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11. A Regulatory Feedback Loop between HIF-1α and PIM2 in HepG2 Cells.
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Yu, Zhenhai, Zhao, Xiaoping, Ge, Yingying, Zhang, Teng, Huang, Liangqian, Zhou, Xiang, Xie, Lei, Liu, Jianjun, and Huang, Gang
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HYPOXIA-inducible factors , *CANCER invasiveness , *CANCER cells , *GLUCOSE metabolism , *SERINE/THREONINE kinases , *MOLECULAR genetics , *GENE expression - Abstract
To survive under hypoxic conditions, cancer cells remodel glucose metabolism to support tumor progression. HIF transcription factor is essential for cellular response to hypoxia. The underlying mechanism how HIF is constitutively activated in cancer cells remains elusive. In the present study, we characterized a regulatory feedback loop between HIF-1α and PIM2 in HepG2 cells. Serine/threonine kinase proto-oncogene PIM2 level was induced upon hypoxia in a HIF-1α-mediated manner in cancer cells. HIF-1α induced PIM2 expression via binding to the hypoxia-responsive elements (HREs) of the PIM2 promoter. In turn, PIM2 interacted with HIF-1α, especially a transactivation domain of HIF-1α. PIM2 as a co-factor but not an upstream kinase of HIF-1α, enhanced HIF-1α effect in response to hypoxia. The positive feedback loop between PIM2 and HIF-1α was correlated with glucose metabolism as well as cell survival in HepG2 cells. Such a regulatory mode may be important for the adaptive responses of cancer cells in antagonizing hypoxia during cancer progression. [ABSTRACT FROM AUTHOR]
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- 2014
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12. Joint Effects of Known Type 2 Diabetes Susceptibility Loci in Genome-Wide Association Study of Singapore Chinese: The Singapore Chinese Health Study.
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Chen, Zhanghua, Pereira, Mark A., Seielstad, Mark, Koh, Woon-Puay, Tai, E. Shyong, Teo, Yik-Ying, Liu, Jianjun, Hsu, Chris, Wang, Renwei, Odegaard, Andrew O., Thyagarajan, Bharat, Koratkar, Revati, Yuan, Jian-Min, Gross, Myron D., and Stram, Daniel O.
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GENETICS of type 2 diabetes , *DISEASE susceptibility , *SINGLE nucleotide polymorphisms , *GENE frequency , *TYPE 2 diabetes risk factors , *GENE mapping , *GENOTYPE-environment interaction - Abstract
Background: Genome-wide association studies (GWAS) have identified genetic factors in type 2 diabetes (T2D), mostly among individuals of European ancestry. We tested whether previously identified T2D-associated single nucleotide polymorphisms (SNPs) replicate and whether SNPs in regions near known T2D SNPs were associated with T2D within the Singapore Chinese Health Study. Methods: 2338 cases and 2339 T2D controls from the Singapore Chinese Health Study were genotyped for 507,509 SNPs. Imputation extended the genotyped SNPs to 7,514,461 with high estimated certainty (r2>0.8). Replication of known index SNP associations in T2D was attempted. Risk scores were computed as the sum of index risk alleles. SNPs in regions ±100 kb around each index were tested for associations with T2D in conditional fine-mapping analysis. Results: Of 69 index SNPs, 20 were genotyped directly and genotypes at 35 others were well imputed. Among the 55 SNPs with data, disease associations were replicated (at p<0.05) for 15 SNPs, while 32 more were directionally consistent with previous reports. Risk score was a significant predictor with a 2.03 fold higher risk CI (1.69–2.44) of T2D comparing the highest to lowest quintile of risk allele burden (p = 5.72×10−14). Two improved SNPs around index rs10923931 and 5 new candidate SNPs around indices rs10965250 and rs1111875 passed simple Bonferroni corrections for significance in conditional analysis. Nonetheless, only a small fraction (2.3% on the disease liability scale) of T2D burden in Singapore is explained by these SNPs. Conclusions: While diabetes risk in Singapore Chinese involves genetic variants, most disease risk remains unexplained. Further genetic work is ongoing in the Singapore Chinese population to identify unique common variants not already seen in earlier studies. However rapid increases in T2D risk have occurred in recent decades in this population, indicating that dynamic environmental influences and possibly gene by environment interactions complicate the genetic architecture of this disease. [ABSTRACT FROM AUTHOR]
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- 2014
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13. Are C-Reactive Protein Associated Genetic Variants Associated with Serum Levels and Retinal Markers of Microvascular Pathology in Asian Populations from Singapore?
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Dorajoo, Rajkumar, Li, Ruoying, Ikram, Mohammad Kamran, Liu, Jianjun, Froguel, Philippe, Lee, Jeannette, Sim, Xueling, Ong, Rick Twee-Hee, Tay, Wan Ting, Peng, Chen, Young, Terri L., Blakemore, Alexandra I. F., Cheng, Ching Yu, Aung, Tin, Mitchell, Paul, Wang, Jie Jin, Klaver, Caroline C., Boerwinkle, Eric, Klein, Ronald, and Siscovick, David S.
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C-reactive protein , *BLOOD serum analysis , *BIOMARKERS , *RETINA , *CARDIOVASCULAR diseases , *COMPUTATIONAL biology , *EPIDEMIOLOGY - Abstract
Introduction: C-reactive protein (CRP) levels are associated with cardiovascular disease and systemic inflammation. We assessed whether CRP-associated loci were associated with serum CRP and retinal markers of microvascular disease, in Asian populations. Methods: Genome-wide association analysis (GWAS) for serum CRP was performed in East-Asian Chinese (N = 2,434) and Malays (N = 2,542) and South-Asian Indians (N = 2,538) from Singapore. Leveraging on GWAS data, we assessed, in silico, association levels among the Singaporean datasets for 22 recently identified CRP-associated loci. At loci where directional inconsistencies were observed, quantification of inter-ethnic linkage disequilibrium (LD) difference was determined. Next, we assessed association for a variant at CRP and retinal vessel traits [central retinal artery equivalent (CRAE) and central retinal vein equivalent (CRVE)] in a total of 24,132 subjects of East-Asian, South-Asian and European ancestry. Results: Serum CRP was associated with SNPs in/near APOE, CRP, HNF1A and LEPR (p-values ≤4.7×10−8) after meta-analysis of Singaporean populations. Using a candidate-SNP approach, we further replicated SNPs at 4 additional loci that had been recently identified to be associated with serum CRP (IL6R, GCKR, IL6 and IL1F10) (p-values ≤0.009), in the Singaporean datasets. SNPs from these 8 loci explained 4.05% of variance in serum CRP. Two SNPs (rs2847281 and rs6901250) were detected to be significant (p-value ≤0.036) but with opposite effect directions in the Singaporean populations as compared to original European studies. At these loci we did not detect significant inter-population LD differences. We further did not observe a significant association between CRP variant and CRVE or CRAE levels after meta-analysis of all Singaporean and European datasets (p-value >0.058). Conclusions: Common variants associated with serum CRP, first detected in primarily European studies, are also associated with CRP levels in East-Asian and South-Asian populations. We did not find a causal link between CRP and retinal measures of microvascular disease. [ABSTRACT FROM AUTHOR]
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- 2013
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14. Genetic Associations of Type 2 Diabetes with Islet Amyloid Polypeptide Processing and Degrading Pathways in Asian Populations.
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Lam, Vincent Kwok Lim, Ma, Ronald Ching Wan, Lee, Heung Man, Hu, Cheng, Park, Kyong Soo, Furuta, Hiroto, Wang, Ying, Tam, Claudia Ha Ting, Sim, Xueling, Ng, Daniel Peng-Keat, Liu, Jianjun, Wong, Tien-Yin, Tai, E. Shyong, Morris, Andrew P., Tang, Nelson Leung Sang, Woo, Jean, Leung, Ping Chung, Kong, Alice Pik Shan, Ozaki, Risa, and Jia, Wei Ping
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GENETICS of type 2 diabetes , *PANCREATIC beta cells , *AMYLOID , *POLYPEPTIDES , *ISLANDS of Langerhans , *AUTOPSY , *OLIGOMERIZATION - Abstract
Type 2 diabetes (T2D) is a complex disease characterized by beta cell dysfunctions. Islet amyloid polypeptide (IAPP) is highly conserved and co-secreted with insulin with over 40% of autopsy cases of T2D showing islet amyloid formation due to IAPP aggregation. Dysregulation in IAPP processing, stabilization and degradation can cause excessive oligomerization with beta cell toxicity. Previous studies examining genetic associations of pathways implicated in IAPP metabolism have yielded conflicting results due to small sample size, insufficient interrogation of gene structure and gene-gene interactions. In this multi-staged study, we screened 89 tag single nucleotide polymorphisms (SNPs) in 6 candidate genes implicated in IAPP metabolism and tested for independent and joint associations with T2D and beta cell dysfunctions. Positive signals in the stage-1 were confirmed by de novo and in silico analysis in a multi-centre unrelated case-control cohort. We examined the association of significant SNPs with quantitative traits in a subset of controls and performed bioinformatics and relevant functional analyses. Amongst the tag SNPs, rs1583645 in carboxypeptidase E (CPE) and rs6583813 in insulin degrading enzyme (IDE) were associated with 1.09 to 1.28 fold increased risk of T2D (PMeta = 9.4×10−3 and 0.02 respectively) in a meta-analysis of East Asians. Using genetic risk scores (GRS) with each risk variant scoring 1, subjects with GRS≥3 (8.2% of the cohort) had 56% higher risk of T2D than those with GRS = 0 (P = 0.01). In a subcohort of control subjects, plasma IAPP increased and beta cell function index declined with GRS (P = 0.008 and 0.03 respectively). Bioinformatics and functional analyses of CPE rs1583645 predicted regulatory elements for chromatin modification and transcription factors, suggesting differential DNA-protein interactions and gene expression. Taken together, these results support the importance of dysregulation of IAPP metabolism in T2D in East Asians. [ABSTRACT FROM AUTHOR]
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- 2013
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15. Natural positive selection and north-south genetic diversity in East Asia.
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Suo, Chen, Xu, Haiyan, Khor, Chiea-Chuen, Ong, Rick TH, Sim, Xueling, Chen, Jieming, Tay, Wan-Ting, Sim, Kar-Seng, Zeng, Yi-Xin, Zhang, Xuejun, Liu, Jianjun, Tai, E-Shyong, Wong, Tien-Yin, Chia, Kee-Seng, and Teo, Yik-Ying
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HUMAN chromosomes , *GENETICS , *GENOMES , *LINKAGE disequilibrium , *HUMAN gene mapping - Abstract
Recent reports have identified a north-south cline in genetic variation in East and South-East Asia, but these studies have not formally explored the basis of these clinical differences. Understanding the origins of these variations may provide valuable insights in tracking down the functional variants in genomic regions identified by genetic association studies. Here we investigate the genetic basis of these differences with genome-wide data from the HapMap, the Human Genome Diversity Project and the Singapore Genome Variation Project. We implemented four bioinformatic measures to discover genomic regions that are considerably differentiated either between two Han Chinese populations in the north and south of China, or across 22 populations in East and South-East Asia. These measures prioritized genomic stretches with: (i) regional differences in the allelic spectrum for SNPs common to the two Han Chinese populations; (ii) differential evidence of positive selection between the two populations as quantified by integrated haplotype score (iHS) and cross-population extended haplotype homozygosity (XP-EHH); (iii) significant correlation between allele frequencies and geographical latitudes of the 22 populations. We also explored the extent of linkage disequilibrium variations in these regions, which is important in combining genetic association studies from North and South Chinese. Two of the regions that emerged are found in HLA class I and II, suggesting that the HLA imputation panel from the HapMap may not be directly applicable to every Chinese sample. This has important implications to autoimmune studies that plan to impute the classical HLA alleles to fine map the SNP association signals. [ABSTRACT FROM AUTHOR]
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- 2012
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16. Four New Families with Autosomal Dominant Partial Epilepsy with Auditory Features: Clinical Description and Linkage to Chromosome 10q24.
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Winawer, Melodie R, Boneschi, Filippo Martinelli, Barker-Cummings, Christie, Lee, Joseph H, Liu, Jianjun, Mekios, Constantine, Gilliam, T. Conrad, Pedley, Timothy A, Hauser, W. Allen, and Ottman, Ruth
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EPILEPSY , *EPIDEMIOLOGY , *GENETICS - Abstract
Summary: Purpose: Autosomal dominant partial epilepsy with auditory features (ADPEAF) is a rare form of nonprogressive lateral temporal lobe epilepsy characterized by partial seizures with auditory disturbances. The gene predisposing to this syndrome was localized to a 10-cM region on chromosome 10q24. We assessed clinical features and linkage evidence in four newly ascertained families with ADPEAF, to refine the clinical phenotype and confirm the genetic localization. Methods: We genotyped 41 individuals at seven microsatellite markers spanning the previously defined 10-cM minimal genetic region. We conducted two-point linkage analysis with the ANALYZE computer package, and multipoint parametric and nonparametric linkage analyses as implemented in GENEHUNTER2. Results: In the four families, the number of individuals with idiopathic epilepsy ranged from three to nine. Epilepsy was focal in all of those with idiopathic epilepsy who could be classified. The proportion with auditory symptoms ranged from 67 to 100%. Other ictal symptoms also were reported; of these, sensory symptoms were most common. Linkage analysis showed a maximum 2-point LOD score of 1.86 at (θ = 0.0 for marker D10S603, and a maximum multipoint LOD score of 2.93. Conclusions: These findings provide strong confirmation of linkage of a gene causing ADPEAF to chromosome 10q24. The results suggest that the susceptibility gene has a differential effect on the lateral temporal lobe, thereby producing the characteristic clinical features described here. Molecular studies aimed at the identification of the causative gene are underway. [ABSTRACT FROM AUTHOR]
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- 2002
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17. ITPKB and ZNF184 are associated with Parkinson's disease risk in East Asians.
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Chew, Elaine Guo Yan, Tan, Louis C.S., Au, Wing-Lok, Prakash, Kumar-M., Liu, Jianjun, Foo, Jia Nee, and Tan, Eng-King
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EAST Asians , *PARKINSON'S disease , *SINGLE nucleotide polymorphisms - Abstract
A recent meta-analysis of Parkinson's disease (PD) genome-wide association studies has identified 17 novel risk loci in the European population. We aim to assess if these reported novel risk loci are similarly implicated in PD risk within the East Asian population by analyzing the reported risk single nucleotide polymorphism or proxy single nucleotide polymorphism in 14,006 East Asian samples (779 patients and 13,227 controls). We found that 9 of the 17 reported novel PD risk loci showed very similar effects in Europeans and East Asians (I2 = 0 to 10.7%), of which 2 loci ITPKB and ZNF184 were significantly associated with PD in our samples. Two of the reported risk loci, ANK2/CAMK2D and CTSB , were non-polymorphic in East Asians and therefore not implicated in PD risk in the East Asian population. Given the small effect sizes of these risk loci, further validation is needed in additional Asian samples. • Tested 17 novel PD risk loci identified by European GWAS in 14,006 East Asian samples. • ITPKB and ZNF184 showed significant association (p < 0.05) with PD in East Asians. • Nine risk loci showed comparable effects between Europeans and East Asians. • Two novel PD risk loci are non-polymorphic in East Asians. • Further validation and meta-analysis with additional Asian samples is needed. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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18. Evaluation of novel Parkinson's disease candidate genes in the Chinese population.
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Chew, Elaine Guo Yan, Liany, Herty, Tan, Louis C.S., Au, Wing-Lok, Prakash, Kumar-M., Annuar, Azlina Ahmad, Chan, Anne Y.Y., Lim, Shen-Yang, Mok, Vincent, Chung, Sun Ju, Song, Kyuyoung, Liu, Jianjun, Foo, Jia Nee, and Tan, Eng-King
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PARKINSON'S disease , *DISEASES - Abstract
Abstract Recent whole-exome sequencing studies in European patients with Parkinson's disease (PD) have identified potential risk variants across 33 novel PD candidate genes. We aim to determine if these reported candidate genes are similarly implicated in Asians by assessing common, rare, and novel nonsynonymous coding variants by sequencing all 33 genes in 198 Chinese samples and genotyping coding variants in an independent set of 9756 Chinese samples. We carried out further targeted sequencing of CD36 in an additional 576 Chinese and Korean samples. We found that only 8 of 43 reported risk variants were polymorphic in our Chinese samples. We identified several heterozygotes for rare loss-of-function mutations, including the reported CD36 p.Gln74Ter variant, in both cases and controls. We also observed 2 potential compound heterozygotes among PD cases for rare loss-of-function mutations in CD36 and SSPO. The other reported variants were common in East Asians and not associated with PD, completely absent, or only found in controls. Therefore, the 33 reported candidate genes and associated variants are unlikely to confer significant PD risk in the East Asian population. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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19. Gene-diet interaction effects on BMI levels in the Singapore Chinese population.
- Author
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Chang, Xuling, Han, Yi, Heng, Chew-Kiat, Dorajoo, Rajkumar, Wang, Ling, Khor, Chiea-Chuen, Liu, Jianjun, Sun, Ye, Sim, Xueling, Tai, E-Shyong, van Dam, Rob M., Koh, Woon-Puay, Yuan, Jian-Min, and Friedlander, Yechiel
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BODY mass index , *DIET , *OBESITY genetics , *OVERWEIGHT persons , *GENETICS , *PHYSIOLOGY - Abstract
Background: Recent genome-wide association studies (GWAS) have identified 97 body-mass index (BMI) associated loci. We aimed to evaluate if dietary intake modifies BMI associations at these loci in the Singapore Chinese population.Methods: We utilized GWAS information from six data subsets from two adult Chinese population (N = 7817). Seventy-eight genotyped or imputed index BMI single nucleotide polymorphisms (SNPs) that passed quality control procedures were available in all datasets. Alternative Healthy Eating Index (AHEI)-2010 score and ten nutrient variables were evaluated. Linear regression analyses between z score transformed BMI (Z-BMI) and dietary factors were performed. Interaction analyses were performed by introducing the interaction term (diet x SNP) in the same regression model. Analysis was carried out in each cohort individually and subsequently meta-analyzed using the inverse-variance weighted method. Analyses were also evaluated with a weighted gene-risk score (wGRS) contructed by BMI index SNPs from recent large-scale GWAS studies.Results: Nominal associations between Z-BMI and AHEI-2010 and some dietary factors were identified (P = 0.047-0.010). The BMI wGRS was robustly associated with Z-BMI (P = 1.55 × 10- 15) but not with any dietary variables. Dietary variables did not significantly interact with the wGRS to modify BMI associations. When interaction analyses were repeated using individual SNPs, a significant association between cholesterol intake and rs4740619 (CCDC171) was identified (β = 0.077, adjPinteraction = 0.043).Conclusions: The CCDC171 gene locus may interact with cholesterol intake to increase BMI in the Singaporean Chinese population, however most known obesity risk loci were not associated with dietary intake and did not interact with diet to modify BMI levels. [ABSTRACT FROM AUTHOR]- Published
- 2018
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20. Linkage disequilibrium mapping of CHEK2: common variation and breast cancer risk.
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Einarsdóttir, Kristjana, Humphreys, Keith, Bonnard, Carine, Palmgren, Juni, Iles, Mark M., Sjölander, Arvid, Yuqing Li, Kee Seng Chia, Liu, Edison T., Hall, Per, Jianjun Liu, Wedrén, Sara, Einarsdóttir, Kristjana, Sjölander, Arvid, Li, Yuqing, Chia, Kee Seng, Liu, Jianjun, and Wedrén, Sara
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BREAST cancer , *CELL cycle , *DNA repair , *GENETIC polymorphisms , *CANCER research , *PROTEIN kinases , *RESEARCH , *GENETICS , *RESEARCH methodology , *ACQUISITION of data , *MEDICAL cooperation , *EVALUATION research , *COMPARATIVE studies , *TRANSFERASES , *DISEASE susceptibility , *POSTMENOPAUSE , *LOGISTIC regression analysis , *BREAST tumors - Abstract
Background: Checkpoint kinase 2 (CHEK2) averts cancer development by promoting cell cycle arrest and activating DNA repair in genetically damaged cells. Previous investigation has established a role for the CHEK2 gene in breast cancer aetiology, but studies have largely been limited to the rare 1100delC mutation. Whether common polymorphisms in this gene influence breast cancer risk remains unknown. In this study, we aimed to assess the importance of common CHEK2 variants on population risk for breast cancer by capturing the majority of diversity in the gene using haplotype tagging single nucleotide polymorphisms (tagSNPs).Methods and Findings: We analyzed 14 common SNPs spanning 52 kilobases (kb) of the CHEK2 gene in 92 Swedish women. Coverage evaluation indicated that these typed SNPs would efficiently convey association signal also from untyped SNPs in the same region. Six of the 14 SNPs predicted well both the haplotypic and single SNP variations within CHEK2. We genotyped these six tagSNPs in 1,577 postmenopausal breast cancer cases and 1,513 population controls, but found no convincing association between any common CHEK2 haplotype and breast cancer risk. The 1100delC mutation was rare in our Swedish population--0.7% in cases and 0.4% in controls--with a corresponding odds ratio for carriers versus noncarriers of 2.26 (95% confidence interval, 0.99-5.15). Estimates of the population frequency and the odds ratio of 1100delC indicate that our sample is representative of a Northern European population.Conclusions: Notwithstanding the involvement of the CHEK2 gene in breast cancer aetiology, we show that common polymorphisms do not influence postmenopausal breast cancer risk. [ABSTRACT FROM AUTHOR]- Published
- 2006
- Full Text
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