1. An Accelerated Analytical Process for the Development of STR Profiles for Casework Samples
- Author
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Nancy Laurin and Chantal J. Frégeau
- Subjects
Quality Control ,Time Factors ,Chromatography ,Biology ,Efficiency, Organizational ,Real-Time Polymerase Chain Reaction ,Bioinformatics ,DNA Fingerprinting ,DNA extraction ,Pathology and Forensic Medicine ,Gene Frequency ,Rapid dna ,Genetics ,Humans ,Microsatellite ,Biological evidence ,Microsatellite Repeats - Abstract
Significant efforts are being devoted to the development of methods enabling rapid generation of short tandem repeat (STR) profiles in order to reduce turnaround times for the delivery of human identification results from biological evidence. Some of the proposed solutions are still costly and low throughput. This study describes the optimization of an analytical process enabling the generation of complete STR profiles (single-source or mixed profiles) for human identification in approximately 5 h. This accelerated process uses currently available reagents and standard laboratory equipment. It includes a 30-min lysis step, a 27-min DNA extraction using the Promega Maxwell(®) 16 System, DNA quantification in
- Published
- 2015
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