Search

Your search keyword '"Terry Furey"' showing total 3 results
Start Over Author "Terry Furey" Topic genetics
3 results on '"Terry Furey"'

3. The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)

Author

Yutaka Suzuki, Stephanie Rodrigues, Francis S. Collins, Agnes Baross, Jane Grimwood, Anna Sneed, Anuradha Madan, Sinnakaruppan Mathavan, Nicole Shapiro, Peggy N. Kwong, Martin Krzywinski, Chia-Lin Wei, Susan Old, Michael R. Brent, Jeff M. Stott, Jim Kent, Robert W. Blakesly, Steven J. Granite, Yaron S.N. Butterfield, Shiraki Toshiyuki, Jessica Fahey, Steven J.M. Jones, Eric D. Green, Jeffery G. Derge, Kirsten Schreiber, Todd E. Scheetz, Anup Madan, C. E. Gruber, Mark Ketteman, Sumio Sugano, Blake A. Simmons, Kathryn A. Makowski, Richard M. Myers, Michael J. Brownstein, David Haussler, Narayan K. Bhat, Minoru S.H. Ko, Thomas L. Casavant, Anca Petrescu, Eduardo Lee, Angela M. Garcia, Obi L. Griffith, David J. Lipman, Terry Furey, Peter J. Good, Koichi Kawakami, Ye Yuan, Jia Qian Wu, Keith Wetherby, Allison M. Peck, Angelique Schnerch, Piero Carninci, Robert A. Holt, Diana L. Palmquist, Amy Sanchez, Stephen L. Johnson, Maria de Fatima Bonaldo, Ursula Skalska, John Douglas Mcpherson, Michelle Whiting, Charles P. Brinkley, Alice C. Young, Elise A. Feingold, Dawood B. Dudekula, M. R. Smith, Joel A. Malek, Christa Prange, Jeremy Schmutz, Wonhee Jang, Richard A. Gibbs, Stephanie Bosak, Alex Rodriguez, Lukas Wagner, Yulan Piao, Mike Feolo, Leonie Misquitta, Donna M. Muzny, Mark S. Guyer, Tom I. Bonner, Florence Hsie, Rebekah S. Rasooly, Nancy Y. Liao, Preethi H. Gunaratne, Malachi Griffith, Troy Moore, Erin Helton, Daniela S. Gerhard, Richard C. Waterman, Ralph F. Hopkins, Kirill Rotmistrovsky, Mark Diekhans, Edwin Fuh, Greg Schuler, Carl F. Schaefer, Anne Hodgson, Gerard G. Bouffard, Sandra W. Clifton, Mark Dickson, Lynette H. Grouse, Tom Driscoll, Stephen W. Hulyk, Susan F. Greenhut, Carolyn M. Shenmen, Steven L. Klein, Duane E. Smailus, Yijun Ruan, Ted B. Usdin, Jacqueline E. Schein, Marco A. Marra, Ryan Morrin, M. Bento Soares, Steven Sherry, Russell L. Pearson, Carla Kowis, and Kenneth H. Buetow

Subjects
clone (Java method), DNA, Complementary, Biology, Mice, Open Reading Frames, Xenopus laevis, Complementary DNA, Genetics, Animals, Humans, Genomic library, Cloning, Molecular, Gene, Genetics (clinical), Zebrafish, DNA Primers, Gene Library, Cloning, cDNA library, Computational Biology, Resources, United States, Rats, Open reading frame, National Institutes of Health (U.S.), Reference genome
Abstract

The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5′-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline.

Published
2004

Catalog

Books, media, physical & digital resources
See catalog results