26 results on '"Zhong, Cheng"'
Search Results
2. The complete mitochondrial genome of the freshwater snail Cipangopaludina ampullacea (Küster, 1852) (Gastropoda: Viviparidae)
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Meng-Meng Wu, Hui-Zhong Cheng, Li-Li Li, and Guang-Long Xie
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caenogastropoda ,viviparoidea ,phylogenetics ,china ,Genetics ,QH426-470 - Abstract
Cipangopaludina ampullacea (Küster, 1852) is a freshwater snail endemic to China. In this study, the complete mitochondrial genome of C. ampullacea was sequenced using next-generation sequencing. The mitogenome is 16,892 bp long and comprises a total of 37 genes, including 13 protein-coding genes, two rRNA genes, and 22 tRNA genes. It is consistent with the basic characteristics of other known viviparid mitochondrial genomes. Phylogenetic analysis using related species mitogenomes showed that Cipangopaludina and Margarya are mutually non monophyletic. Our study provides valuable information to reconstruct the taxonomy and evolution of viviparid snails more comprehensively.
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- 2022
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3. Extracellular vesicles derived from human Sertoli cells: characterizations, proteomic analysis, and miRNA profiling
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Xiao-Hui Tan, Sheng-Ji Gu, Wen-Jie Tian, Wen-Peng Song, Yang-Yang Gu, Yi-Ming Yuan, Xue-Song Li, Zhong-Cheng Xin, Sae Woong Kim, Rui-Li Guan, and Woong Jin Bae
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Male ,Proteomics ,Extracellular Vesicles ,MicroRNAs ,Sertoli Cells ,Genetics ,Humans ,Proteins ,General Medicine ,Molecular Biology ,Chromatography, Liquid ,Molecular Chaperones - Abstract
Extracellular vesicles (EVs) contain thousands of proteins and nucleic acids, playing an important role in cell-cell communications. Sertoli cells have been essential in the testis as a "nurse cell". However, EVs derived from human Sertoli cells (HSerCs) have not been well investigated.EVs were isolated from HSerCs via ultracentrifugation and characterized by transmission electron microscopy, tunable resistive pulse sensing, and Western blotting. The cargo carried by HSerCs-EVs was measured via liquid chromatography-mass spectrometry and GeneChip miRNA Arrays. Bioinformatic analysis was performed to reveal potential functions of HSerCs-EVs.A total of 860 proteins with no less than 2 unique peptides and 88 microRNAs with high signal values were identified in HSerCs-EVs. Biological processes related to molecular binding, enzyme activity, and regulation of cell cycle were significantly enriched. Specifically, many proteins in HSerCs-EVs were associated with spermatogenesis and regulation of immune system, including Septins, Large proline-rich protein BAG6, Clusterin, and Galectin-1. Moreover, abundant microRNAs within HSerCs-EVs (miR-638, miR-149-3p, miR-1246, etc.) had a possible impact on male reproductive disorders such as asthenozoospermia and oligozoospermia.Our study has shown that HSerCs-EVs contain diverse components such as proteins and microRNAs. Further research is required to evaluate HSerCs-EVs in spermatogenesis, which are underutilized but highly potent resources with particular promise for male infertility.
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- 2022
4. Characterization of Endolithic Culturable Microbial Communities in Carbonate Rocks from a Typical Karst Canyon in Guizhou (China)
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Yuan Tang, Jian-Zhong Cheng, and Bin Lian
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endolithic bacteria ,endolithic fungi ,carbonate rock ,Genetics ,QH426-470 ,Microbiology ,QR1-502 - Abstract
The endolithic environment is a ubiquitous habitat for microorganisms and a critical interface between biology and geology. In this study, a culture-based method and the phylogenetic analysis based on 16S rRNA and internal transcribed spacer (ITS) sequences were used to investigate the diversity of endolithic bacteria and fungi in two main types of carbonate rocks (namely dolomite and limestone) from Nanjiang Canyon in Guizhou karst area, China. The results of bacterial diversity indicated that all bacteria isolated from dolomite and limestone rocks were divided into 4 bacterial groups, including Proteobacteria, Actinobacteria, Firmicutes and Bacteroidetes. For these two kinds of rocks, Proteobacteria was the first dominant group, and Gammaproteobacteria occupied the greatest proportion which might be closely related to Pseudomonas in phylogeny to be the most dominant genera after isolation. Actinobacteria and Bacillus bacteria were also widespread in these two kinds of rock environments. There were only 9 and 8 strains of fungi isolated from dolomite and limestone respectively, which all belonged to Ascomycota. To the best of our knowledge, this is the first report on diversity of endolithic culturable bacteria and fungi in carbonate rocks in Guizhou karst region. These microorganisms may play an important and unprecedented role in the carbonate rock weathering during the long history of geological evolution.
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- 2016
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5. Mitochondrial Dysfunction in Polycystic Ovary Syndrome
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Shuang Lian Long, Qin Huang, Xin Zeng, Qiaoqing Zhong, and Zhong-Cheng Mo
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0301 basic medicine ,Mitochondrial DNA ,medicine.medical_specialty ,DNA Copy Number Variations ,endocrine system diseases ,Mitochondrion ,Biology ,03 medical and health sciences ,0302 clinical medicine ,Insulin resistance ,Internal medicine ,Genetics ,medicine ,Humans ,Genetic Predisposition to Disease ,Molecular Biology ,Dyslipidemias ,Endocrine disease ,nutritional and metabolic diseases ,Type 2 Diabetes Mellitus ,Cell Biology ,General Medicine ,medicine.disease ,Polycystic ovary ,female genital diseases and pregnancy complications ,Mitochondria ,030104 developmental biology ,Endocrinology ,Diabetes Mellitus, Type 2 ,030220 oncology & carcinogenesis ,Hypertension ,Mutation ,Female ,Insulin Resistance ,Metabolic syndrome ,Hyperandrogenism ,Dyslipidemia ,Polycystic Ovary Syndrome - Abstract
Polycystic ovary syndrome (PCOS) is one of the most common female reproductive metabolisms. It is an endocrine disease that affects reproductive women and often exhibits with hyperandrogenemia, insulin resistance (IR), low inflammation, and an increased risk of type 2 diabetes mellitus, metabolic syndrome, and cardiovascular events such as hypertension and dyslipidemia in patients. However, the molecular mechanism of PCOS is still unclear. Recently, an increasing number of studies have shown that the oxidative stress induced by mitochondrial dysfunction has negative effects on IR, lipid metabolism, and follicular development, suggesting that mitochondrial dysfunction plays an essential role in the development of PCOS. Abnormal mitochondrial DNA copy number in patients with PCOS, and mitochondrial gene mutations, has been the focus of research in recent years, and functional mitochondrial diseases have been gradually accepted as a related factor in PCOS. This review is intended to summarize and discuss previous and recent studies and findings on the connections between mitochondrial dysfunction and PCOS.
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- 2020
6. Dendrobium nobile Lindl polysaccharides improve testicular spermatogenic function in streptozotocin-induced diabetic rats
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Xiaocan Lei, Peng Huo, Yuan‐jie Xie, Yaohui Wang, Guanghai Liu, Haoyan Tu, Qingxiang Shi, Zhong‐cheng Mo, and Shun Zhang
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Blood Glucose ,Male ,Cell Biology ,Metformin ,Streptozocin ,Diabetes Mellitus, Experimental ,Rats ,Sirtuin 1 ,Polysaccharides ,Proliferating Cell Nuclear Antigen ,Testis ,Genetics ,Sperm Motility ,Animals ,Dendrobium ,Developmental Biology - Abstract
Dendrobium nobile Lindl polysaccharides (DNLP) exhibited various biological functions. This study aimed to investigate the protective effects of DNLP on testicular spermatogenic function in streptozotocin (STZ)-induced diabetic rats in comparison with metformin. The blood glucose level was significantly increased and the homeostatic model assessment for insulin resistance (HOMA-IR) aggravated markedly in diabetic rats. The weight of testis and epididymis, and the sperm number and motility were decreased in the diabetic rats. The pathologic changes occurred in the spermatogenic tubules along with the decreased number of spermatogenic cells, downregulated proliferating cell nuclear antigen (PCNA) and Sirtuin 1 (SIRT1) expression and increased cell apoptosis in the testes. Compared with the model group, DNLP and metformin treatment significantly decreased the level of blood glucose, improved the HOMA-IR, and increased the weight of testis and epididymis, as well as the sperm number and sperm motility. Furthermore, the pathologic changes in the spermatogenic tubules improved significantly with increased number of spermatogenic cells, the upregulation of PCNA and SIRT1 and suppression of cell apoptosis in the testes. Collectively, our study for the first time examined the effects of DNLP on the male reproductive system of STZ-induced diabetic rats, and indicated that DNLP was protective against diabetes mellitus-induced testis injury via increasing the proliferation, inhibiting cell apoptosis and upregulating SIRT1 expression in testicular spermatogenic cells.
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- 2021
7. Role of microRNA in the Pathogenesis of Polycystic Ovary Syndrome
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Hongliang Wu, Zhuo Chen, Zhong-Cheng Mo, Hanxiao Ou, and Peng Wu
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0301 basic medicine ,Infertility ,endocrine system diseases ,Biology ,Bioinformatics ,03 medical and health sciences ,0302 clinical medicine ,Insulin resistance ,Ovarian Follicle ,Diabetes mellitus ,microRNA ,Genetics ,medicine ,Humans ,Obesity ,Molecular Biology ,Dyslipidemias ,Ovary ,Hyperandrogenism ,Cancer ,Cell Biology ,General Medicine ,medicine.disease ,Polycystic ovary ,female genital diseases and pregnancy complications ,MicroRNAs ,030104 developmental biology ,Gene Expression Regulation ,030220 oncology & carcinogenesis ,Androgens ,Female ,Insulin Resistance ,Metabolic syndrome ,Biomarkers ,Polycystic Ovary Syndrome - Abstract
Polycystic ovary syndrome (PCOS) is the most typical metabolic syndrome in women of reproductive age, with a high prevalence and an increased risk of long-term complications. PCOS mainly manifests as hyperandrogenism (HA), ovulatory dysfunction, and polycystic ovaries, in addition to being relevant to infertility, insulin resistance (IR), obesity, lipid abnormalities, and chronic low-grade inflammation. The etiology of this syndrome remains largely unknown. microRNAs (miRNAs), small, noncoding RNAs (nearly 22 nucleotides long), regulate gene expression at the posttranscriptional level. Abnormal miRNA levels are closely associated with the occurrence of diseases, such as diabetes, cancers, and atherosclerosis, and miRNAs can be used as predictors and diagnostic biomarkers for cancer. Interestingly, the roles of miRNAs in PCOS pathology have attracted considerable attention in recent years. Research has established that alterations in miRNA expression in women with PCOS compared with healthy women may act as noninvasive biomarkers and new therapeutic targets in PCOS. This article aims to summarize the latest research on the relationship between miRNAs and the clinical manifestations of PCOS while also providing a few mechanisms based on previous studies. Understanding the relationship between miRNAs and PCOS will provide guidance for researchers to further explore the complexity and heterogeneity of PCOS.
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- 2019
8. Effect of dietary zinc pectin oligosaccharides chelate on growth performance, enzyme activities, Zn accumulation, metallothionein concentration, and gene expression of Zn transporters in broiler chickens1
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Hu Cui, Tietao Zhang, Hao Nie, Hui Min Yu, Xiu Hua Gao, Jing Jing Xie, and Zhong Cheng Wang
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Male ,food.ingredient ,Pectin ,Animal feed ,Oligosaccharides ,chemistry.chemical_element ,Zinc ,Superoxide dismutase ,Random Allocation ,03 medical and health sciences ,Animal science ,food ,Gene expression ,Genetics ,Animals ,Metallothionein ,Pancreas ,030304 developmental biology ,chemistry.chemical_classification ,Minerals ,0303 health sciences ,biology ,Chemistry ,Body Weight ,0402 animal and dairy science ,Broiler ,Membrane Transport Proteins ,04 agricultural and veterinary sciences ,General Medicine ,Animal Feed ,040201 dairy & animal science ,Diet ,Non ruminant Nutrition ,Enzyme ,Gene Expression Regulation ,Liver ,Dietary Supplements ,biology.protein ,Pectins ,Female ,Animal Science and Zoology ,Soybeans ,Chickens ,Food Science - Abstract
This study was to investigate the effect of zinc pectin oligosaccharides chelate (Zn-POS) on growth performance, serum enzyme activities, tissue zinc accumulation, metallothionein (MT) concentrations, and gene expression of zinc transporters (ZnT) in broilers. Five hundred forty 1-d-old Arbor Acres broiler chicks were randomly assigned to 5 dietary groups with 6 replicates of 18 birds per replicate. The diets were formulated with the same supplemental Zn level (80 mg/kg diet) but different amount of the Zn-POS: 0, 200, 400, 600, and 800 mg Zn-POS/kg diet. ZnSO(4) was used to adjust to the desired amount of the Zn (80 mg/kg) in the Zn-POS diets. Broilers were fed with the experimental diets for 42 d including the starter (days 1 to 21) and grower (days 22 to 42) phases. Our results showed that dietary supplementation of Zn-POS linearly and quadratically increased (P < 0.05) the average daily gain and gain-to-feed ratio during 22 to 42 d and 1 to 42 d as well as body weight on day 42, whereas reduced (P < 0.05) the sum of mortality and lag abnormalities in broilers on day 42. Besides, serum alkaline phosphatase and copper-zinc superoxide dismutase activities increased (P < 0.05) linearly and quadratically in response to dietary Zn-POS supplemental level on day 42. Dietary Zn-POS supplementation increased Zn accumulation in serum (linear, P < 0.05), liver (linear, P < 0.05), and pancreas (linear and quadratic, P < 0.05). In addition, Zn-POS supplementation linearly and quadratically increased (P < 0.01, P < 0.05, respectively) MT concentrations in liver and pancreas of broilers. Pancreatic mRNA levels of MT, ZnT-1, and ZnT-2 increased (P < 0.05) linearly and quadratically, and the mRNA expression of metal response element-binding transcription factor-1 increased linearly (P < 0.05), in response to dietary Zn-POS supplementation. In conclusion, supplementation of Zn-POS in the diet increases Zn enrichment in the metabolic organs such as liver and pancreas and promotes productive performance in broilers.
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- 2019
9. eNOS-NO-induced small blood vessel relaxation requires EHD2-dependent caveolae stabilization
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Matthaeus, Claudia, Lian, Xiaoming, Kunz, Séverine, Lehmann, Martin, Zhong, Cheng, Bernert, Carola, Lahmann, Ines, Müller, Dominik N., Gollasch, Maik, and Daumke, Oliver
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Cancer Research ,Cell Membranes ,Biochemistry ,Cytosol ,Medicine and Health Sciences ,Small interfering RNAs ,Staining ,Cell Staining ,Arteries ,Animal Models ,Mesenteric Arteries ,Nucleic acids ,Vasodilation ,Experimental Organism Systems ,Medicine ,Cellular Structures and Organelles ,Anatomy ,Technology Platforms ,Function and Dysfunction of the Nervous System ,Research Article ,Nitric Oxide Synthase Type III ,Science ,Mouse Models ,Research and Analysis Methods ,Caveolae ,Nitric Oxide ,Model Organisms ,Physical Conditioning, Animal ,Genetics ,Human Umbilical Vein Endothelial Cells ,Animals ,Humans ,Non-coding RNA ,Cell Membrane ,Biology and Life Sciences ,Membrane Proteins ,Cell Biology ,Gene regulation ,Mice, Inbred C57BL ,Coated Pits ,Specimen Preparation and Treatment ,Cardiovascular and Metabolic Diseases ,Cardiovascular Anatomy ,Animal Studies ,Blood Vessels ,RNA ,Calcium ,Gene expression ,Carrier Proteins - Abstract
Endothelial nitric oxide synthase (eNOS)-related vessel relaxation is a highly coordinated process that regulates blood flow and pressure and is dependent on caveolae. Here, we investigated the role of caveolar plasma membrane stabilization by the dynamin-related ATPase EHD2 on eNOS-nitric oxide (NO)-dependent vessel relaxation. Loss of EHD2 in small arteries led to increased numbers of caveolae that were detached from the plasma membrane. Concomitantly, impaired relaxation of mesenteric arteries and reduced running wheel activity were observed in EHD2 knockout mice. EHD2 deletion or knockdown led to decreased production of nitric oxide (NO) although eNOS expression levels were not changed. Super-resolution imaging revealed that eNOS was redistributed from the plasma membrane to internalized detached caveolae in EHD2-lacking tissue or cells. Following an ATP stimulus, reduced cytosolic Ca(2+) peaks were recorded in human umbilical vein endothelial cells (HUVECs) lacking EHD2. Our data suggest that EHD2-controlled caveolar dynamics orchestrates the activity and regulation of eNOS/NO and Ca(2+) channel localization at the plasma membrane.
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- 2019
10. Neurite Outgrowth Inhibitor B Receptor: A Versatile Receptor with Multiple Functions and Actions
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Shuang-Lian Long, Yuan-jie Xie, Yu-Kun Li, Zhi-Feng Long, Jin-Feng Shi, and Zhong-Cheng Mo
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Vascular Endothelial Growth Factor A ,0301 basic medicine ,Nervous system ,Glycosylation ,Nogo Receptors ,Neurite ,Angiogenesis ,Nogo Proteins ,Lipid Metabolism Disorders ,Vesicular Transport Proteins ,Neovascularization, Physiologic ,Apoptosis ,Receptors, Cell Surface ,Biology ,03 medical and health sciences ,Neoplasms ,mental disorders ,Genetics ,medicine ,Animals ,Humans ,Receptor ,Molecular Biology ,Cell Proliferation ,Glycoproteins ,Dolichol Phosphates ,Endoplasmic reticulum ,Niemann-Pick Disease, Type C ,Chemotaxis ,Cell Biology ,General Medicine ,Lipid Metabolism ,Cell biology ,030104 developmental biology ,medicine.anatomical_structure ,Reticulon ,Carrier Proteins ,psychological phenomena and processes ,Signal Transduction - Abstract
Members of the reticulon protein family are predominantly distributed within the endoplasmic reticulum. The neurite outgrowth inhibitor (Nogo) has three subtypes, including Nogo-A (200 kDa), Nogo-B (55 kDa), and Nogo-C (25 kDa). Nogo-A and Nogo-C are potent Nogos that are predominantly expressed in the central nervous system. Nogo-B, the splice variant of reticulon-4, is expressed widely in multiple human organ systems, including the liver, lung, kidney, blood vessels, and inflammatory cells. Moreover, the Nogo-B receptor (NgBR) can interact with Nogo-B and can independently affect nervous system regeneration, the chemotaxis of endothelial cells, proliferation, and apoptosis. In recent years, it has been demonstrated that NgBR plays an important role in human pathophysiological processes, including lipid metabolism, angiogenesis, N-glycosylation, cell apoptosis, chemoresistance in human hepatocellular carcinoma, and epithelial-mesenchymal transition. The pathophysiologic effects of NgBR have garnered increased attention, and the detection and enhancement of NgBR expression may be a novel approach to monitor the development and to improve the prognosis of relevant human clinical diseases.
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- 2017
11. Characterization of cholesterol metabolism in Sertoli cells and spermatogenesis
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Kun Ren, Jin Feng Shi, Zhong Cheng Mo, Yuan‑Jie Xie, Wei‑Dong Yin, and Yu‑Kun Li
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0301 basic medicine ,Male ,Cancer Research ,endocrine system ,Review ,Biology ,Biochemistry ,03 medical and health sciences ,Paracrine signalling ,chemistry.chemical_compound ,Sterol 14-Demethylase ,0302 clinical medicine ,Desmosterol ,Testis ,Genetics ,medicine ,Humans ,Spermatogenesis ,Molecular Biology ,Sertoli Cells ,urogenital system ,Gene Expression Regulation, Developmental ,Cell cycle ,Sertoli cell ,Lipid Metabolism ,Epithelium ,Sterol ,Cell biology ,030104 developmental biology ,medicine.anatomical_structure ,Cholesterol ,Oncology ,chemistry ,030220 oncology & carcinogenesis ,cholesterol metabolism ,Molecular Medicine ,lipids (amino acids, peptides, and proteins) ,A431 cells - Abstract
The Sertoli cell, which is the supporting cell of spermatogenesis, has an important role in the endocrine and paracrine control of spermatogenesis. Functionally, it provides the cells of the seminiferous epithelium with nutrition, conveys mature spermatids to the lumen of seminiferous tubules, secretes androgen‑binding protein and interacts with endocrine Leydig cells. In addition, the levels of cholesterol, as well as its intermediates, vary greatly between nongonadal tissues and the male reproductive system. Throughout spermatogenesis, a dynamic and constant alteration in the membrane lipid composition of Sertoli cells occurs. In several mammalian species, testis meiosis‑activating sterol and desmosterol, as well as other cholesterol precursors, accumulate in the testes and spermatozoa. In addition, certain cholesterogenic genes exhibit stage‑specific expression patterns during spermatogenesis, including the cytochrome P450 enzyme lanosterol 14α‑demethylase. Inconsistency in the patterns of gene expression during spermatogenesis indicates a cell‑type specific and complex temporary modulation of lipids and cholesterol, which also implicates the dynamic interactions between Sertoli cells and germ cells. Furthermore, in the female reproductive tract and during epididymal transit, which is a prerequisite for valid fertilization, the modulation of cholesterol occurring in spermatozoal membranes further indicates the functional importance of sterol compounds in spermatogenesis. However, the exact role of cholesterol metabolism in Sertoli cells in sperm production is unknown. The present review article describes the progress made in the research regarding the characteristics of the Sertoli cell, particularly the regulation of its cholesterol metabolism during spermatogenesis.
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- 2017
12. Association of pre-eclampsia with SOD2 Ala16Val polymorphism among mother-father-infant triads.
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Luo, Zhong‐Cheng, Julien, Pierre, Wei, Shu‐Qin, Audibert, Francois, Fraser, William D., the Maternal and Infant Research on Oxidative Stress (MIROS) study group, Luo, Zhong-Cheng, Wei, Shu-Qin, and Maternal and Infant Research on Oxidative Stress (MIROS) study group
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PREECLAMPSIA prevention , *PREECLAMPSIA , *GENETIC polymorphisms , *MOTHER-infant relationship , *URBAN hospitals , *ANTIOXIDANTS , *GENETICS , *DISEASE susceptibility , *FATHERS , *LONGITUDINAL method , *MOTHERS , *SALIVA , *SUPEROXIDE dismutase , *CITY dwellers , *GENOTYPES - Abstract
Objective: To determine whether pre-eclampsia is associated with polymorphisms in superoxide dismutase (SOD) genes among mother-father-infant triads.Methods: We did this follow-up cohort study at 17 urban hospitals in Canada between October 1, 2008, and September 30, 2010. We recruited Canadian participants who had participated in the International Trial of Antioxidant Supplementation for the Prevention of Pre-eclampsia. Saliva specimens were collected for DNA extraction. The SOD1 +35A/C (rs2234694) and SOD2 Ala16Val C/T (rs4880) single-nucleotide polymorphisms (SNPs) were genotyped.Results: Dual presence of the SOD2 Ala16Val TT variant among mother-father pairs (n=657) was associated with an increased risk of pre-eclampsia when compared with the absence of the TT variant among the mother-father pairs (7/48 [14.6%] vs 11/339 [3.2%]; adjusted odds ratio 6.80, 95% confidence interval 2.32-19.95; P<0.001). By contrast, presence of a single T variant in mother-father pairs (16/270 [5.9%]) or mother-infant pairs (8/179 [4.5%]) was not associated with pre-eclampsia. The SOD1 +35A/CSNP was not associated with pre-eclampsia.Conclusion: The SOD2 Ala16Val SNP might be involved in paternal influence on the maternal predisposition to pre-eclampsia. Genotyping of mother-father pairs could be a promising strategy to identify pre-eclampsia genes. [ABSTRACT FROM AUTHOR]- Published
- 2018
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13. Structural Basis of Human Transcription Factor Sry-related Box 17 Binding to DNA
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Yong Xie, Hai Gao, Nana Gao, Zhong Cheng, Wei Jiang, Shu-Yi Si, and Huolian Qian
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Models, Molecular ,endocrine system ,animal structures ,HMG-box ,Protein Conformation ,Base pair ,Molecular Sequence Data ,Static Electricity ,Biology ,Crystallography, X-Ray ,Biochemistry ,Mice ,Structural Biology ,SOXF Transcription Factors ,Animals ,Humans ,Protein–DNA interaction ,Amino Acid Sequence ,chemistry.chemical_classification ,Genetics ,DNA ligase ,Binding Sites ,DNA clamp ,Sequence Homology, Amino Acid ,Hydrogen Bonding ,DNA ,General Medicine ,DNA-binding domain ,Cell biology ,DNA binding site ,chemistry ,HMG-Box Domains ,embryonic structures ,DNA supercoil ,Protein Binding - Abstract
Sry-related box (Sox) transcription factors share a conserved high-mobility-group box domain (HMG-domain) that binds DNA in the minor groove and bends DNA for further assembly of transcriptional machineries. During organogenesis, each member of the Sox family triggers a specific cell lineage differentiation, indicating that their interactions with DNA are different from each other. Therefore, investigating structural rearrangement of each Sox transcription factor HMG-domain upon binding to DNA would help to elucidate the distinctive molecular mechanism by which they interact with DNA. Previous studies have determined the crystal structures of Sox2 HMG-domain/DNA, Sox4 HMGdomain/ DNA, Sox9 HMG-domain/DNA and Sox17 HMG-domain/DNA complexes. However, major gaps remain in the structural information on the Sox transcription factor HMG-domains. Here, we report the crystal structure of the human Sox17 HMG-domain alone at 2.4 A resolution. Comparing this structure and the structure of the mouse Sox17 HMGdomain/ DNA complex provides structural understanding of the mechanism of Sox17 binding to DNA. Specifically, after electrostatic interactions attract Sox17 to DNA, Asn73, Ser99, and Trp106 form hydrogen bonds with DNA, Arg70, Lys80, Arg83, His94, and Asn95 on Sox17 undergo conformational changes and form hydrogen bonds with DNA, contributing to the electrostatic interaction between Sox17 and DNA.
- Published
- 2013
14. Is Cesarean Delivery Preferable in Twin Pregnancies at >=36 Weeks Gestation?
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Jun Zhang, Zhong-Cheng Luo, Lu Chen, Hong Huang, Yu Dong, Ware Branch, Yu-Na Guo, and Zujing Yang
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Physiology ,Maternal Health ,Twins ,lcsh:Medicine ,Blood Pressure ,Vascular Medicine ,Labor and Delivery ,0302 clinical medicine ,Pregnancy ,Infant Mortality ,Medicine and Health Sciences ,Birth Weight ,030212 general & internal medicine ,lcsh:Science ,Twin Pregnancy ,030219 obstetrics & reproductive medicine ,Multidisciplinary ,Vaginal delivery ,Obstetrics ,Pregnancy Outcome ,Gestational age ,Obstetrics and Gynecology ,Survival Rate ,Physiological Parameters ,Obstetric Procedures ,Hypertension ,Gestation ,Female ,Research Article ,Adult ,medicine.medical_specialty ,Birth weight ,Surgical and Invasive Medical Procedures ,Gestational Age ,03 medical and health sciences ,Young Adult ,Hypertensive Disorders in Pregnancy ,Intensive care ,medicine ,Genetics ,Humans ,Retrospective Studies ,Gynecology ,business.industry ,Cesarean Section ,Body Weight ,lcsh:R ,Infant, Newborn ,Biology and Life Sciences ,Neonates ,Infant ,Human Genetics ,medicine.disease ,Delivery, Obstetric ,Health Care ,Birth ,Intensive Care, Neonatal ,Pregnancy, Twin ,Women's Health ,Multiple birth ,lcsh:Q ,Health Statistics ,Morbidity ,business ,Developmental Biology - Abstract
Background The optimal mode of delivery in twin pregnancies remains controversial. A recent randomized trial did not find any benefit of planned cesarean vs. vaginal delivery at 32–38 weeks gestation, but the trial was not powered to detect a moderate effect. We aimed to evaluate the impact of cesarean delivery on perinatal mortality and severe neonatal morbidity in twin pregnancies at ≥32 weeks through a large database exploration approach with the power to detect moderate risk differences. Methods In a retrospective birth cohort study using the U.S. matched multiple births, 1995–2000 (the available largest multiple birth dataset), we compared perinatal outcomes in twins (n = 181,810 pregnancies) delivered at 32–41 weeks gestation without congenital anomalies. The primary outcome was a composite of perinatal death and severe neonatal morbidity. Cox regression was used to estimate the adjusted hazard ratio (aHR) controlling for the propensity to cesarean delivery, fetal characteristics (sex, birth weight, birth weight discordance, same-sex twin or not) and twin-cluster level dependence. Prospective risks were calculated using the fetuses-at-risk denominators. Results The overall rates of the primary outcome were slightly lower in intended cesarean (6.20%) vs. vaginal (6.45%) deliveries. The aHRs of the primary outcome were in favor of vaginal delivery at 32 (aHR = 1.06, p = 0.03) or 33 (aHR = 1.22, p
- Published
- 2016
15. Uncv (uncovered): a new mutation causing hairloss on mouse chromosome 11
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Yong-Fu Chen, Bao-Sheng Ge, Shan-Ru Li, Xiu-Ling Yu, Ji-Qing Li, Cui-E Wang, Yi-Fan Lu, Shi-En Zhu, Dong-Ping Wang, Run-Fang Li, Zhong-Cheng Zhang, and Na Wu
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Genetics ,Coat ,Genetic marker ,Mutant ,Microsatellite ,Locus (genetics) ,General Medicine ,Biology ,Phenotype ,Gene ,Recombination Fraction - Abstract
A pair of mutant mice with a first sparse coat appeared spontaneously in the production stock of BALB/c mice with a normal coat. After being sib-mated, they produced three phenotypes in their progeny: mice with normal hair, mice with a first sparse coat and then a fuzzy coat, and uncovered mice. Genetic studies revealed the mutants had inherited an autosomal monogene that was semi-dominant. By using 11 biochemical loci – Idh, Car2, Mup1, Pgm1, Hbb, Es1, Es10, Gdc, Ce2, Mod1 and Es3 – as genetic markers, two-point linkage tests were made. The results showed the gene was assigned to chromosome 11. The result of a three-point test with Es3 and D11Mit8 (microsatellite DNA) as markers showed that the mutation was linked to Es3 with the recombination fraction 7·89±2·19%, and linked to D11Mit8 with the recombination fraction 26·30±3·57%. The recombination fraction between Es3 and D11Mit8 was 32·90±3·81%. It is suggested that the mutation is a new genetic locus that affected the skin and hair structure of the mouse. The mutation was named uncovered, with the symbol Uncv. Further studies showed the mutation affected not only the histology of skin and hair but also the growth and reproductive performance of the mice. The molecular characterization of the Uncv locus needs to be further studied.
- Published
- 1999
16. Gene Therapy of Parkinson Disease Model Rat by Direct Injection of Plasmid DNA–Lipofectin Complex
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Zhong-Cheng Zheng, Chang-Fu Zhou, Lei Cao, Zhi-Hua Jiang, Sheng-Di Chen, Xin-Yuan Liu, Zhen-Guo Liu, and Ying-Chun Zhao
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Transcription, Genetic ,Tyrosine 3-Monooxygenase ,Genetic enhancement ,Molecular Sequence Data ,Nigrostriatal pathway ,Striatum ,Biology ,Polymerase Chain Reaction ,Injections ,Plasmid ,Genetics ,medicine ,Animals ,Molecular Biology ,Gene ,DNA Primers ,Base Sequence ,Behavior, Animal ,Tyrosine hydroxylase ,Phosphatidylethanolamines ,Dopaminergic ,Gene Transfer Techniques ,Parkinson Disease ,Genetic Therapy ,Immunohistochemistry ,Molecular biology ,Rats ,Reverse transcription polymerase chain reaction ,Disease Models, Animal ,medicine.anatomical_structure ,Molecular Medicine ,Plasmids - Abstract
Lipofectin-mediated gene transfer was used to introduce plasmid harboring the tyrosine hydroxylase (TH) gene into the striatum of rats with lesions of the nigrostriatal pathway. The rotational asymmetry of Parkinson disease model rat was reduced quickly and significantly, suggesting that plasmid–DNA-transfected brain cells can generate l-dopa locally in the striatum in quantities sufficient to compensate partially for the loss of intrinsic striatal dopaminergic input. Immunohistochemical staining and reverse transcription polymerase chain reaction (RT-PCR) also confirm that striatal cells can express exogenous TH gene. Such in vivo plasmid DNA transfer strategy may be useful in other neurologic disease therapy, especially acute brain insults.
- Published
- 1995
17. Histone Methyltransferase Enhancer of Zeste Homolog 2-Mediated ABCA1 Promoter DNA Methylation Contributes to the Progression of Atherosclerosis
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Xi-Long Zheng, Wei Xie, Feng Yao, Xiangyang Tang, Jin-Feng Shi, Wei Wan, Jian-Feng Wu, Chao-Ke Tang, Ping Zhang, Ping-Ping He, Xin-Ping Ouyang, Yun-Cheng Lv, Juan Peng, Francisco S. Cayabyab, Dan Liu, Yan-Yan Tang, and Zhong-Cheng Mo
- Subjects
Male ,0301 basic medicine ,lcsh:Medicine ,030204 cardiovascular system & hematology ,Biochemistry ,Vascular Medicine ,Epigenesis, Genetic ,Mice ,White Blood Cells ,0302 clinical medicine ,Animal Cells ,Histone methylation ,Medicine and Health Sciences ,polycyclic compounds ,lcsh:Science ,Promoter Regions, Genetic ,Aorta ,Epigenomics ,Regulation of gene expression ,DNA methylation ,Multidisciplinary ,EZH2 ,hemic and immune systems ,Lipids ,Chromatin ,Nucleic acids ,Cholesterol ,Histone methyltransferase ,Epigenetics ,lipids (amino acids, peptides, and proteins) ,Cellular Types ,Anatomy ,DNA modification ,Chromatin modification ,ATP Binding Cassette Transporter 1 ,Research Article ,Chromosome biology ,Cell biology ,Immune Cells ,Immunology ,Down-Regulation ,Biology ,DNA methyltransferase ,Cell Line ,03 medical and health sciences ,Apolipoproteins E ,Epigenetics of physical exercise ,Genetics ,Animals ,Humans ,Enhancer of Zeste Homolog 2 Protein ,cardiovascular diseases ,Blood Cells ,Biology and life sciences ,Macrophages ,lcsh:R ,nutritional and metabolic diseases ,DNA ,Atherosclerosis ,Molecular biology ,Mice, Inbred C57BL ,RAW 264.7 Cells ,030104 developmental biology ,Cardiovascular Anatomy ,Blood Vessels ,lcsh:Q ,Gene expression ,Gene Deletion - Abstract
ATP-binding cassette transporter A1 (ABCA1) plays a critical role in maintaining cellular cholesterol homeostasis. The purpose of this study is to identify the molecular mechanism(s) underlying ABCA1 epigenetic modification and determine its potential impact on ABCA1 expression in macrophage-derived foam cell formation and atherosclerosis development. DNA methylation induced foam cell formation from macrophages and promoted atherosclerosis in apolipoprotein E-deficient (apoE-/-) mice. Bioinformatics analyses revealed a large CpG island (CGI) located in the promoter region of ABCA1. Histone methyltransferase enhancer of zeste homolog 2 (EZH2) downregulated ABCA1 mRNA and protein expression in THP-1 and RAW264.7 macrophage-derived foam cells. Pharmacological inhibition of DNA methyltransferase 1 (DNMT1) with 5-Aza-dC or knockdown of DNMT1 prevented the downregulation of macrophage ABCA1 expression, suggesting a role of DNA methylation in ABCA1 expression. Polycomb protein EZH2 induced DNMT1 expression and methyl-CpG-binding protein-2 (MeCP2) recruitment, and stimulated the binding of DNMT1 and MeCP2 to ABCA1 promoter, thereby promoting ABCA1 gene DNA methylation and atherosclerosis. Knockdown of DNMT1 inhibited EZH2-induced downregulation of ABCA1 in macrophages. Conversely, EZH2 overexpression stimulated DNMT1-induced ABCA1 gene promoter methylation and atherosclerosis. EZH2-induced downregulation of ABCA1 gene expression promotes foam cell formation and the development of atherosclerosis by DNA methylation of ABCA1 gene promoter.
- Published
- 2016
18. Epigallocatechin-3-gallate prevents TNF-α-induced NF-κB activation thereby upregulating ABCA1 via the Nrf2/Keap1 pathway in macrophage foam cells
- Author
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Xin-Ping Ouyang, Yun-Cheng Lv, Jin Jiang, Kai Yin, Chao-Ke Tang, Guo-Jun Zhao, Yuchang Fu, Zhong-Cheng Mo, and Zhi-Sheng Jiang
- Subjects
NF-E2-Related Factor 2 ,Anti-Inflammatory Agents ,complex mixtures ,Catechin ,Cell Line ,Downregulation and upregulation ,Genetics ,Humans ,Liver X receptor ,Receptor ,Kelch-Like ECH-Associated Protein 1 ,biology ,Tumor Necrosis Factor-alpha ,Reverse cholesterol transport ,Intracellular Signaling Peptides and Proteins ,NF-kappa B ,food and beverages ,Lipid metabolism ,General Medicine ,KEAP1 ,Cell biology ,Up-Regulation ,Cholesterol ,Apoptosis ,ABCA1 ,Cancer research ,biology.protein ,lipids (amino acids, peptides, and proteins) ,ATP-Binding Cassette Transporters ,ATP Binding Cassette Transporter 1 ,Foam Cells ,Signal Transduction - Abstract
The ATP-binding membrane cassette transporter A1 (ABCA1) plays a protective role in the development of atherosclerosis for the reverse cholesterol transport process. Epigallocatechin-3-gallate (EGCG), which exists abundantly in green tea, exerts an anti-atherosclerotic effect via anti-inflammatory and metabolic regulation activities. Many genes and proteins related to lipid metabolism are involved in the lowering cholesterol effects of EGCG. However, effects of EGCG on ABCA1 have rarely been described. In the study presented here, we found that exposure of macrophage foam cells to TNF-α results in a downregulation of ABCA1 and a decrease in cholesterol efflux to apoA1, which is attenuated by pretreatment with EGCG. Moreover, rather than activating the Liver X receptor (LXR) pathway, inhibition of the TNF-α-induced nuclear factor-κB (NF-κB) activity is detected with EGCG treatment in cells. In order to inhibit the NF-κB activity, EGCG can promote the dissociation of the nuclear factor E2-related factor 2 (Nrf2)-Kelch-like ECH-associated protein 1 (Keap1) complex; when the released Nrf2 translocates to the nucleus and activates the transcription of genes containing an ARE element inhibition of NF-κB occurs and Keap1 is separated from the complex to directly interact with IKKβ and thus represses NF-κB function. These results provide novel insight into the anti-inflammatory effects of EGCG, as well as the identification of a novel potential therapeutic role for the prevention of atherosclerosis.
- Published
- 2011
19. MicroRNA-203 inhibits cell proliferation by repressing ΔNp63 expression in human esophageal squamous cell carcinoma
- Author
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Xiaohong Liu, Zhi-Yong Zeng, Shengdong Huang, Yang Yuan, Dejun Gong, Jing Tao, and He-Zhong Cheng
- Subjects
Cancer Research ,Esophageal Neoplasms ,Blotting, Western ,Biology ,lcsh:RC254-282 ,Surgical oncology ,RNA interference ,Cell Line, Tumor ,microRNA ,Genetics ,Humans ,Doubling time ,Luciferases ,3' Untranslated Regions ,Transcription factor ,Cell Proliferation ,Reverse Transcriptase Polymerase Chain Reaction ,Three prime untranslated region ,Cell growth ,Tumor Suppressor Proteins ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Molecular biology ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,Oncology ,Carcinoma, Squamous Cell ,Trans-Activators ,Cancer research ,RNA Interference ,Stem cell ,Transcription Factors ,Research Article - Abstract
Background This study was performed to investigate the effect of microRNA-203 (miR-203) and ΔNp63 on cell proliferation and the functional connection between miR-203 and ΔNp63 in ESCC. Methods We employed 2 human ESCC cell lines, Eca109 and TE-1, as the model system. The effect of miR-203 and ΔNp63 on cell proliferation was determined in cells transfected with miR-203 mimic and ΔNp63 small interfering RNA (siRNA), respectively. The regulation of ΔNp63 expression in ESCC cells by miR-203 was studied by luciferase reporter assay, RT-PCR and western blot analysis in cells transfected with miR-203. The effect of ΔNp63 re-expression on miR-203 induced inhibition of cell proliferation was studied by cell proliferation assay in cells cotransfected with miR-203 and pcDNA-ΔNp63 plasmid (without the 3'-UTR of ΔNp63). Results We found that both miR-203 and ΔNp63 siRNA signicantly inhibited cell proliferation in ESCC. MiR-203 could down-regulate endogenous ΔNp63 expression at the posttranscriptional level. Moreover, re-expression of ΔNp63 in cells transfected with miR-203 significantly attenuated the miR-203 induced inhibition of cell proliferation. Conclusions Our data implied that miR-203 could inhibit cell proliferation in human ESCC through ΔNp63-mediated signal pathway. Therefore, we propose that miR-203 might be used as a therapeutic agent for human ESCC.
- Published
- 2011
20. A Method for Finding Metabolic Pathways Using Atomic Group Tracking.
- Author
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Huang, Yiran, Zhong, Cheng, Lin, Hai Xiang, and Wang, Jianyi
- Subjects
- *
INFORMATION theory , *THERMODYNAMICS , *GIBBS' free energy , *GLYCOLYSIS , *BIOINFORMATICS - Abstract
A fundamental computational problem in metabolic engineering is to find pathways between compounds. Pathfinding methods using atom tracking have been widely used to find biochemically relevant pathways. However, these methods require the user to define the atoms to be tracked. This may lead to failing to predict the pathways that do not conserve the user-defined atoms. In this work, we propose a pathfinding method called AGPathFinder to find biochemically relevant metabolic pathways between two given compounds. In AGPathFinder, we find alternative pathways by tracking the movement of atomic groups through metabolic networks and use combined information of reaction thermodynamics and compound similarity to guide the search towards more feasible pathways and better performance. The experimental results show that atomic group tracking enables our method to find pathways without the need of defining the atoms to be tracked, avoid hub metabolites, and obtain biochemically meaningful pathways. Our results also demonstrate that atomic group tracking, when incorporated with combined information of reaction thermodynamics and compound similarity, improves the quality of the found pathways. In most cases, the average compound inclusion accuracy and reaction inclusion accuracy for the top resulting pathways of our method are around 0.90 and 0.70, respectively, which are better than those of the existing methods. Additionally, AGPathFinder provides the information of thermodynamic feasibility and compound similarity for the resulting pathways. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
21. 337: SOD2 gene Ala16Val polymorphism in mother-father-baby trios is associated with preeclampsia
- Author
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Zhong-Cheng Luo, Pierre Julien, Shu-Qin Wei, François Audibert, and William D. Fraser
- Subjects
Genetics ,business.industry ,medicine ,SOD2 ,Obstetrics and Gynecology ,medicine.disease ,business ,Gene ,Preeclampsia - Published
- 2014
22. Arbuscular mycorrhizal status of plants and the spore density of arbuscular mycorrhizal fungi in the tropical rain forest of Xishuangbanna, southwest China
- Author
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Guo-Hua Wang, Yong-Mei Xia, Li-Zhong Cheng, Zhi-Wei Zhao, Xin-Zheng Qin, Tao Sha, and Xi-Wu Li
- Subjects
Rhizosphere ,biology ,Plant Science ,General Medicine ,Rainforest ,biology.organism_classification ,Spore ,Arbuscular mycorrhiza ,Symbiosis ,Botany ,Genetics ,Acaulospora ,Mycorrhiza ,Molecular Biology ,Ecology, Evolution, Behavior and Systematics ,Glomus - Abstract
The arbuscular mycorrhizal status of 112 plant species and the spore density of arbuscular mycorrhizal fungi (AMF) in the rhizosphere soil of these plants in the tropical rain forest of Xishuangbanna, southwest China, were surveyed. It was found that 56% of the surveyed species were arbuscular mycorrhizal, 31% were possibly arbuscular mycorrhizal and 13% were non-mycorrhizal. The spore density of AMF ranged from 55 to 1,908 per 100 g soil, with an average of 476. The rhizosphere soil from the arbuscular mycorrhizal plants did not always have a higher AMF spore density than that from the possibly mycorrhizal and non-mycorrhizal plants. The clumped distribution of AMF spores and the complex structure of the underground component of the tropical rain forest may be two important factors that affected the spore density of AMF. Fungi belonging to the genera Acaulospora and Glomus are the dominant AMF in the soil of the tropical rain forest of Xishuangbanna.
- Published
- 2001
23. Histone Methyltransferase Enhancer of Zeste Homolog 2-Mediated ABCA1 Promoter DNA Methylation Contributes to the Progression of Atherosclerosis.
- Author
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Lv, Yun-Cheng, Tang, Yan-Yan, Zhang, Ping, Wan, Wei, Yao, Feng, He, Ping-Ping, Xie, Wei, Mo, Zhong-Cheng, Shi, Jin-Feng, Wu, Jian-Feng, Peng, Juan, Liu, Dan, Cayabyab, Francisco S., Zheng, Xi-Long, Tang, Xiang-Yang, Ouyang, Xin-Ping, and Tang, Chao-Ke
- Subjects
HISTONE methyltransferases ,ATHEROSCLEROSIS ,GENE enhancers ,DNA methylation ,PROMOTERS (Genetics) ,DISEASE progression ,ATP-binding cassette transporters - Abstract
ATP-binding cassette transporter A1 (ABCA1) plays a critical role in maintaining cellular cholesterol homeostasis. The purpose of this study is to identify the molecular mechanism(s) underlying ABCA1 epigenetic modification and determine its potential impact on ABCA1 expression in macrophage-derived foam cell formation and atherosclerosis development. DNA methylation induced foam cell formation from macrophages and promoted atherosclerosis in apolipoprotein E-deficient (apoE
−/− ) mice. Bioinformatics analyses revealed a large CpG island (CGI) located in the promoter region of ABCA1. Histone methyltransferase enhancer of zeste homolog 2 (EZH2) downregulated ABCA1 mRNA and protein expression in THP-1 and RAW264.7 macrophage-derived foam cells. Pharmacological inhibition of DNA methyltransferase 1 (DNMT1) with 5-Aza-dC or knockdown of DNMT1 prevented the downregulation of macrophage ABCA1 expression, suggesting a role of DNA methylation in ABCA1 expression. Polycomb protein EZH2 induced DNMT1 expression and methyl-CpG-binding protein-2 (MeCP2) recruitment, and stimulated the binding of DNMT1 and MeCP2 to ABCA1 promoter, thereby promoting ABCA1 gene DNA methylation and atherosclerosis. Knockdown of DNMT1 inhibited EZH2-induced downregulation of ABCA1 in macrophages. Conversely, EZH2 overexpression stimulated DNMT1-induced ABCA1 gene promoter methylation and atherosclerosis. EZH2-induced downregulation of ABCA1 gene expression promotes foam cell formation and the development of atherosclerosis by DNA methylation of ABCA1 gene promoter. [ABSTRACT FROM AUTHOR]- Published
- 2016
- Full Text
- View/download PDF
24. Is Cesarean Delivery Preferable in Twin Pregnancies at >=36 Weeks Gestation?
- Author
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Dong, Yu, Luo, Zhong-Cheng, Yang, Zu-Jing, Chen, Lu, Guo, Yu-Na, Branch, Ware, Zhang, Jun, and Huang, Hong
- Subjects
- *
CESAREAN section , *DELIVERY (Obstetrics) , *PERINATAL death , *HUMAN abnormalities , *PREGNANCY complications , *CLINICAL trials - Abstract
Background: The optimal mode of delivery in twin pregnancies remains controversial. A recent randomized trial did not find any benefit of planned cesarean vs. vaginal delivery at 32–38 weeks gestation, but the trial was not powered to detect a moderate effect. We aimed to evaluate the impact of cesarean delivery on perinatal mortality and severe neonatal morbidity in twin pregnancies at ≥32 weeks through a large database exploration approach with the power to detect moderate risk differences. Methods: In a retrospective birth cohort study using the U.S. matched multiple births, 1995–2000 (the available largest multiple birth dataset), we compared perinatal outcomes in twins (n = 181,810 pregnancies) delivered at 32–41 weeks gestation without congenital anomalies. The primary outcome was a composite of perinatal death and severe neonatal morbidity. Cox regression was used to estimate the adjusted hazard ratio (aHR) controlling for the propensity to cesarean delivery, fetal characteristics (sex, birth weight, birth weight discordance, same-sex twin or not) and twin-cluster level dependence. Prospective risks were calculated using the fetuses-at-risk denominators. Results: The overall rates of the primary outcome were slightly lower in intended cesarean (6.20%) vs. vaginal (6.45%) deliveries. The aHRs of the primary outcome were in favor of vaginal delivery at 32 (aHR = 1.06, p = 0.03) or 33 (aHR = 1.22, p<0.001) weeks, neutral at 34–35 weeks, but in favor of cesarean delivery at 36 (aHR = 0.94, p = 0.004), 37, 38 and 39+ weeks (aHR: 0.72 to 0.78, all p<0.001). The lower risk of the primary outcome for cesarean vs. vaginal deliveries at 36+ weeks of gestation remained when the analyses were restricted to different-sex (dichorionic) twins (aHR = 0.84, 95% CI 0.80–0.88). Conclusion: Cesarean delivery may be beneficial for perinatal outcomes overall in twin pregnancies at ≥36 weeks gestation. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
25. Development of microsatellite markers in an amphicarpic species, Amphicarpaea edgeworthii Benth. (Leguminosae).
- Author
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Zhong-Cheng Liang, Ji Yang, and Guang-Yuan Rao
- Subjects
- *
MICROSATELLITE repeats , *CHROMOSOMES , *LEGUMES , *SEEDS , *GENETICS - Abstract
Eight polymorphic simple sequence repeats (SSRs) markers were developed for Amphicarpaea edgeworthii Benth., an amphicarpic species in East Asia. The low level of heterozygosity in populations and the high level of population differentiation found in this study suggest that A. edgeworthii has a mixed mating system that is predominantly selfing. The findings also indicate that the new markers can be used in genetic analyses of the mating system and the estimation of the construction of progeny populations contributed by chasmogamously and cleistogamously produced seeds. [ABSTRACT FROM AUTHOR]
- Published
- 2007
- Full Text
- View/download PDF
26. 607: Baby's vitamin D receptor gene polymorphisms is associated with preterm birth.
- Author
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Wei, Shuqin, Bi, Wei Guang, Leduc, Line, Nuyt, Anne Monique, Audibert, Francois, Luo, Zhong Cheng, Julien, Pierre, and Fraser, William
- Subjects
PREMATURE labor ,VITAMIN D receptors ,SINGLE nucleotide polymorphisms ,ANTIOXIDANTS ,CHEMILUMINESCENCE immunoassay ,GENETICS - Published
- 2017
- Full Text
- View/download PDF
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