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Your search keyword '"microRNA‐30a‐3p"' showing total 3 results
Start Over Descriptor "microRNA‐30a‐3p" Topic genetics
3 results on '"microRNA‐30a‐3p"'

1. MicroRNA-30a-3p overexpression improves sepsis-induced cell apoptosis in vitro and in vivo via the PTEN/PI3K/AKT signaling pathway.

Author

Yang, Shuying, Wang, Yongqiang, Gao, Hongmei, and Wang, Bing

Subjects
*SEPSIS, *GENETIC overexpression, *MICRORNA, *APOPTOSIS, *PROTEIN expression, *PHOSPHOINOSITIDES, *GENETICS
Abstract

The aim of the present study was to explain the mechanism of miR-30a-3p overexpression in sepsis-induced cell apoptosis in vitro and in vivo. For the in vitro cell experiments, H9c2 cells were divided into three groups, including the untreated normal control (NC), lipopolysaccharide (LPS)-treated and miRNA (treated with LPS and transfection with miRNA-30a-3p) groups. The cell proliferation and apoptosis were evaluated by MTT assay and flow cytometry, respectively. The relative protein expression levels were measured by western blot assay. In the in vivo experiment, a sepsis rat model was established by intraperitoneal injection of LPS. Sprague Dawley rats were divided into three groups, including the NC, LPS-injected and miRNA (in which model rats were injected with miR-30a-3p vector at the caudal vein) groups. The myocardial morphology in different groups was observed by hematoxylin and eosin staining. In addition, tissue apoptosis and protein expression levels were evaluated by TUNEL and western blot assay, respectively. The results of cell experiments indicated that the cell proliferation rate was significantly increased and the cell apoptosis rate was signifi- cantly downregulated in the miR-30a-3p group compared with the LPS group (both P<0.05). The relative protein expression of phosphatase and tensin homolog (PTEN) was markedly decreased in the miRNA group compared with the LPS group, while the levels of phosphoinositide 3-kinase (PI3K) and protein kinase B (AKT) were significantly increased in the miRNA group (all P<0.05). In the in vivo experiments, the myocardial morphology of the miRNA group was improved compared with that of the LPS group. Compared with the LPS group, cell apoptosis in the miRNA group was significantly downregulated (P<0.05), while the relative protein levels (PTEN, PI3K and AKT) in the tissues were also significantly altered (P<0.05). In conclusion, miR-30a-3p overexpression may improve the sepsis-induced cell apoptosis in vitro and in vivo via the PTEN/PI3K/AKT signaling pathway. [ABSTRACT FROM AUTHOR]

Published
2018
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2. Hypoxia‐sensitive LINC01436 is regulated by E2F6 and acts as an oncogene by targeting miR‐30a‐3p in non‐small cell lung cancer

Author

Gang Meng, Chengying Li, Meiyu Zhou, Qingyun Liu, Zeyao Hu, Weijia Xie, Long Wu, Zubin Yu, Xiangyu Ma, Na Wu, Tong-Jian Cai, Yafei Li, Li Bai, Ying Xiang, Yao Zhang, Guilu Wang, and Shuai Yuan

Subjects
0301 basic medicine, Male, Cancer Research, Lung Neoplasms, E2F6 Transcription Factor, Proto-Oncogene Mas, Metastasis, 0302 clinical medicine, Cell Movement, Carcinoma, Non-Small-Cell Lung, Basic Helix-Loop-Helix Transcription Factors, Neoplasm Metastasis, Research Articles, Mice, Inbred BALB C, EPAS1, General Medicine, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Long non-coding RNA, Up-Regulation, Gene Expression Regulation, Neoplastic, Oncology, 030220 oncology & carcinogenesis, Molecular Medicine, RNA, Long Noncoding, Research Article, non‐small cell lung cancer, Mice, Nude, Biology, lcsh:RC254-282, Models, Biological, 03 medical and health sciences, In vivo, Cell Line, Tumor, microRNA, Genetics, medicine, Animals, Humans, Neoplasm Invasiveness, long noncoding RNA, Lung cancer, Cell Proliferation, microRNA‐30a‐3p, Oncogene, Base Sequence, hypoxia, Oncogenes, LINC01436, medicine.disease, Survival Analysis, In vitro, MicroRNAs, 030104 developmental biology, Cancer research, Tumor Hypoxia
Abstract

Dysregulation of long noncoding RNA (lncRNA) is known to be involved in numerous human diseases, including lung cancer. However, the precise biological functions of most lncRNA remain to be elucidated. Here, we identified a novel up-regulated lncRNA, LINC01436 (RefSeq: NR_110419.1), in non-small cell lung cancer (NSCLC). High expression of LINC01436 was significantly associated with poor overall survival. Notably, LINC01436 expression was transcriptionally repressed by E2F6 under normoxia, and the inhibitory effect was relieved in a hypoxic microenvironment. Gain- and loss-of-function studies revealed that LINC01436 acted as a proto-oncogene by promoting lung cancer cell growth, migration and invasion in vitro. Xenograft tumor assays in nude mice confirmed that LINC01436 promoted tumor growth and metastasis in vivo. Mechanistically, LINC01436 exerted biological functions by acting as a microRNA (miR)-30a-3p sponge to regulate the expression of its target gene EPAS1. Our findings characterize LINC01436 as a new hypoxia-sensitive lncRNA with oncogenic function in NSCLC, suggesting that LINC01436 may be a potential biomarker for prognosis and a potential target for treatment.

Published
2019

3. MiRNA‑30a‑3p inhibits metastasis and enhances radiosensitivity in esophageal carcinoma by targeting insulin‑like growth factor 1 receptor

Author

Jing Wu, Qian Zhang, Yan-Xin Fan, Pudong Qian, Xiuhua Bian, Yan-Hong Luo, Pengwei Yan, and Jing Wen

Subjects
Male, 0301 basic medicine, Cancer Research, Epithelial-Mesenchymal Transition, Esophageal Neoplasms, Carcinogenesis, medicine.medical_treatment, Cell, Radiation Tolerance, Biochemistry, Receptor, IGF Type 1, esophageal carcinoma, 03 medical and health sciences, 0302 clinical medicine, Downregulation and upregulation, Cell Movement, Cell Line, Tumor, microRNA, Genetics, medicine, metastasis, Humans, Neoplasm Invasiveness, Radiosensitivity, Insulin-Like Growth Factor I, Neoplasm Metastasis, Molecular Biology, Aged, Cell Proliferation, Oncogene, Chemistry, Growth factor, Carcinoma, Articles, microRNA-30a-3p, Middle Aged, Cell cycle, Gene Expression Regulation, Neoplastic, 030104 developmental biology, medicine.anatomical_structure, Oncology, insulin-like growth factor 1 receptor, radiosensitivity, Cell culture, 030220 oncology & carcinogenesis, Cancer research, Molecular Medicine, Female
Abstract

It has been demonstrated that microRNAs (miRNAs) serve important roles in various biological processes, such as tumorigenesis. In the present study, the role of miR‑30a‑3p in the pathogenesis of esophageal carcinoma (EC) was investigated. Reverse transcription‑quantitative polymerase chain reaction was performed to determine the levels of miR‑30a‑3p expression in EC tissues and cell lines. Then, the effects of miR‑30a‑3p on the migration, invasion and radiosensitivity of EC cells were investigated using scratch‑wound, Transwell and radiosensitivity assays, respectively. A dual‑luciferase reporter assay was performed to determine potential interactions between miR‑30a‑3p and the 3'‑untranslated region (3'‑UTR) of insulin‑like growth factor 1 receptor (IGF‑1R). The results demonstrated that the levels of miR‑30a‑3p expression in EC tissues and cell lines were significantly decreased compared with those in paired healthy tissues and a human esophageal epithelial cell line. Upregulation of miR‑30a‑3p expression significantly suppressed migration, invasion and epithelial‑mesenchymal transition (EMT), and enhanced radiosensitivity in EC cells. Analysis of luciferase activity demonstrated that miR‑30a‑3p interacted with the 3'‑UTR of IGF‑1R, and knockdown of IGF‑1R induced similar effects on the migration, invasion, EMT and radiosensitivity of EC cells. The results indicated that miR‑30a‑3p suppressed metastasis and enhanced the radiosensitivity of EC cells via downregulation IGF‑1R, suggesting that miR‑30a‑3p may be a potential therapeutic target in the treatment of EC.

Published
2019
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