1. Clostridium polynesiense sp. nov., a new member of the human gut microbiota in French Polynesia.
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Sankar, Senthil Alias, Rathored, Jaishriram, Metidji, Sarah, Lagier, Jean-Christophe, Khelaifia, Saber, Labas, Noemie, Musso, Didier, Raoult, Didier, and Fournier, Pierre-Edouard
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CLOSTRIDIUM , *HUMAN microbiota , *PHYSIOLOGIC strain , *ANAEROBIC bacteria , *GRAM-positive bacteria , *GENOMES , *PHENOTYPES , *PHYSIOLOGY - Abstract
Strain MS1, a Gram-positive, obligately anaerobic, motile and spore-forming rod belonging to the Clostridium genus, was isolated from the feces of a healthy Polynesian male living in French Polynesia. The temperature range for growth was 30–45 °C. We sequenced its complete genome and studied its phenotypic characteristics. The 3,560,738-bp long genome (one chromosome, no plasmid, G + C content 34%) contained 3535 protein-coding and 70 RNA genes. Strain MS1 exhibited a 98.24% 16S rRNA similarity with Clostridium amylolyticum , the phylogenetically closest species. When compared with other Clostridium species with standing in nomenclature, it had an average genomic similarity of 68.8–70%, a unique MALDI-TOF spectrum, and differed in nitrate reduction, motility and l -arabinose and d -lactose metabolism with most of the closest species. Therefore, strain MS1 is sufficiently distinct from type strains of the genus Clostridium to represent a novel species within this genus, for which the name Clostridium polynesiense sp. nov. is proposed. The type strain of C. polynesiense is MS1 T (= CSUR P630 = DSM 27072). [ABSTRACT FROM AUTHOR]
- Published
- 2015
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