Search

The largemouth bass belongs to the family Centrarchidae, which includes two subspecies: the northern subspecies, Micropterus salmoides salmoides, and the Florida subspecies, Micropterus salmoides floridanus. In this study, the complete mitochondrial genomes of the two subspecies were sequenced, and their genetic differences were identified. The mitogenomes of M. s. salmoides and M. s. floridanus are 16,486 and 16,479 bp in length, respectively. The two subspecies consisted of 37 genes (13 protein-coding genes, 2 ribosomal RNA, and 22 transfer RNA), which are typical for vertebrate mtDNA. Phylogenetic analysis provided statistical support for the monophyly of the family Centrarchidae. Comparison of the two subspecies' mitogenomes revealed a relatively high number (450) of single nucleotide polymorphisms (SNPs) in protein-coding genes. We characterized SNPs in the partial cytochrome c oxidase subunit 1 gene of different individuals from three cultured populations, one wild northern subspecies population, and one wild Florida subspecies population. Twenty-eight SNPs were fixed with alternative nucleotides in the two subspecies, which could be used for differentiating them. Based on this gene, phylogenetic tree and genetic distance analyses supported that cultured largemouth bass in China belongs to the northern subspecies. [ABSTRACT FROM AUTHOR]

Background and aims. The swordtail (Xiphophorus helleri) has been used in studies of behavior, phylogenetics, genetics, physiology, cell biology, cancer research, and biomedicine. Mitochondrial DNA (mtDNA) studies are of pressing need in order to assess the population history of the species. Materials and method. In this study, we present the complete mtDNA genome sequence of two specimens: one from a RR-B strain, a 27-generation inbred line; and one non-selective swordtail with red eyes and red body color from the Guangzhou market, measuring 16,638 and 16,635 bp, respectively. Results. The genome comprises 13 protein-coding genes, 22 tRNAs, two rRNAs and a major non-coding region. The comparison of the two specimens' mitogenomes revealed a relatively low number (57) of single nucleotide polymorphisms—29 located in protein-coding genes, 11 in rRNA genes, six in tRNA genes, and six in the non-coding region. Conclusion. We present an important genetic resource for the RR-B strain of swordtails and swordtail species in general. [ABSTRACT FROM AUTHOR]