1. A functional genomics screen identifying blood cell development genes in Drosophila by undergraduates participating in a course-based research experience.
- Author
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Evans CJ, Olson JM, Mondal BC, Kandimalla P, Abbasi A, Abdusamad MM, Acosta O, Ainsworth JA, Akram HM, Albert RB, Alegria-Leal E, Alexander KY, Ayala AC, Balashova NS, Barber RM, Bassi H, Bennion SP, Beyder M, Bhatt KV, Bhoot C, Bradshaw AW, Brannigan TG, Cao B, Cashell YY, Chai T, Chan AW, Chan C, Chang I, Chang J, Chang MT, Chang PW, Chang S, Chari N, Chassiakos AJ, Chen IE, Chen VK, Chen Z, Cheng MR, Chiang M, Chiu V, Choi S, Chung JH, Contreras L, Corona E, Cruz CJ, Cruz RL, Dang JM, Dasari SP, De La Fuente JRO, Del Rio OMA, Dennis ER, Dertsakyan PS, Dey I, Distler RS, Dong Z, Dorman LC, Douglass MA, Ehresman AB, Fu IH, Fua A, Full SM, Ghaffari-Rafi A, Ghani AA, Giap B, Gill S, Gill ZS, Gills NJ, Godavarthi S, Golnazarian T, Goyal R, Gray R, Grunfeld AM, Gu KM, Gutierrez NC, Ha AN, Hamid I, Hanson A, Hao C, He C, He M, Hedtke JP, Hernandez YK, Hlaing H, Hobby FA, Hoi K, Hope AC, Hosseinian SM, Hsu A, Hsueh J, Hu E, Hu SS, Huang S, Huang W, Huynh M, Javier C, Jeon NE, Ji S, Johal J, John A, Johnson L, Kadakia S, Kakade N, Kamel S, Kaur R, Khatra JS, Kho JA, Kim C, Kim EJ, Kim HJ, Kim HW, Kim JH, Kim SA, Kim WK, Kit B, La C, Lai J, Lam V, Le NK, Lee CJ, Lee D, Lee DY, Lee J, Lee J, Lee J, Lee JY, Lee S, Lee TC, Lee V, Li AJ, Li J, Libro AM, Lien IC, Lim M, Lin JM, Liu CY, Liu SC, Louie I, Lu SW, Luo WY, Luu T, Madrigal JT, Mai Y, Miya DI, Mohammadi M, Mohanta S, Mokwena T, Montoya T, Mould DL, Murata MR, Muthaiya J, Naicker S, Neebe MR, Ngo A, Ngo DQ, Ngo JA, Nguyen AT, Nguyen HCX, Nguyen RH, Nguyen TTT, Nguyen VT, Nishida K, Oh SK, Omi KM, Onglatco MC, Almazan GO, Paguntalan J, Panchal M, Pang S, Parikh HB, Patel PD, Patel TH, Petersen JE, Pham S, Phan-Everson TM, Pokhriyal M, Popovich DW, Quaal AT, Querubin K, Resendiz A, Riabkova N, Rong F, Salarkia S, Sama N, Sang E, Sanville DA, Schoen ER, Shen Z, Siangchin K, Sibal G, Sin G, Sjarif J, Smith CJ, Soeboer AN, Sosa C, Spitters D, Stender B, Su CC, Summapund J, Sun BJ, Sutanto C, Tan JS, Tan NL, Tangmatitam P, Trac CK, Tran C, Tran D, Tran D, Tran V, Truong PA, Tsai BL, Tsai PH, Tsui CK, Uriu JK, Venkatesh S, Vo M, Vo NT, Vo P, Voros TC, Wan Y, Wang E, Wang J, Wang MK, Wang Y, Wei S, Wilson MN, Wong D, Wu E, Xing H, Xu JP, Yaftaly S, Yan K, Yang E, Yang R, Yao T, Yeo P, Yip V, Yogi P, Young GC, Yung MM, Zai A, Zhang C, Zhang XX, Zhao Z, Zhou R, Zhou Z, Abutouk M, Aguirre B, Ao C, Baranoff A, Beniwal A, Cai Z, Chan R, Chien KC, Chaudhary U, Chin P, Chowdhury P, Dalie J, Du EY, Estrada A, Feng E, Ghaly M, Graf R, Hernandez E, Herrera K, Ho VW, Honeychurch K, Hou Y, Huang JM, Ishii M, James N, Jang GE, Jin D, Juarez J, Kesaf AE, Khalsa SK, Kim H, Kovsky J, Kuang CL, Kumar S, Lam G, Lee C, Lee G, Li L, Lin J, Liu J, Ly J, Ma A, Markovic H, Medina C, Mungcal J, Naranbaatar B, Patel K, Petersen L, Phan A, Phung M, Priasti N, Ruano N, Salim T, Schnell K, Shah P, Shen J, Stutzman N, Sukhina A, Tian R, Vega-Loza A, Wang J, Wang J, Watanabe R, Wei B, Xie L, Ye J, Zhao J, Zimmerman J, Bracken C, Capili J, Char A, Chen M, Huang P, Ji S, Kim E, Kim K, Ko J, Laput SLG, Law S, Lee SK, Lee O, Lim D, Lin E, Marik K, Mytych J, O'Laughlin A, Pak J, Park C, Ryu R, Shinde A, Sosa M, Waite N, Williams M, Wong R, Woo J, Woo J, Yepuri V, Yim D, Huynh D, Wijiewarnasurya D, Shapiro C, Levis-Fitzgerald M, Jaworski L, Lopatto D, Clark IE, Johnson T, and Banerjee U
- Subjects
- Animals, Blood Cells, Humans, Students, Drosophila genetics, Genomics education, Universities
- Abstract
Undergraduate students participating in the UCLA Undergraduate Research Consortium for Functional Genomics (URCFG) have conducted a two-phased screen using RNA interference (RNAi) in combination with fluorescent reporter proteins to identify genes important for hematopoiesis in Drosophila. This screen disrupted the function of approximately 3500 genes and identified 137 candidate genes for which loss of function leads to observable changes in the hematopoietic development. Targeting RNAi to maturing, progenitor, and regulatory cell types identified key subsets that either limit or promote blood cell maturation. Bioinformatic analysis reveals gene enrichment in several previously uncharacterized areas, including RNA processing and export and vesicular trafficking. Lastly, the participation of students in this course-based undergraduate research experience (CURE) correlated with increased learning gains across several areas, as well as increased STEM retention, indicating that authentic, student-driven research in the form of a CURE represents an impactful and enriching pedagogical approach., (© The Author(s) 2021. Published by Oxford University Press on behalf of Genetics Society of America.)
- Published
- 2021
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