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A new virulent Acinetobacter phage, BUCT629 (GenBank no. MZ712044.1), was isolated from hospital sewage. Next-generation sequencing (NGS) results demonstrated that the double-stranded linear DNA genome of phage BUCT629 is 46,325 bp in length with a G+C content of 38%. The BLASTn analysis showed that the genome sequence shared similarity with Acinetobacter phage vB_AbaM_IME285, with 65% query coverage and 98.23% identity, suggesting that phage BUCT629 is a novel phage. The phage genome contains 89 putative protein-coding genes, and no rRNA or tRNA genes were identified. The results of this study may be helpful for discovering new antibacterial agents and for understanding the evolution and genetic diversity of Acinetobacter phages. [ABSTRACT FROM AUTHOR]

Enterobacter hormaechei is an opportunistic pathogen and is found in a large variety of food including animal-derived food. In recent years, bacteria present a severe clinical challenge due to their increasing resistance to antibiotics. Bacteriophages have gained attention as a new antibacterial strategy. In this study, we isolated a novel E. hormaechei bacteriophage IME278 from hospital sewage in Beijing, China. Bacteriophage IME278 had a double-stranded linear DNA genome with 40,164 bp and 51.99% GC content. Whole-genome alignments showed IME278 shared 87% homology with other phages in the National Center for Biotechnology Information (NCBI) database. And phylogenetic analysis demonstrated that IME278 was highly similar to bacteriophages belonging to the genus Kayfunavirus , family Autographiviridae, indicating IME278 can be classified as a new member of the Autographiviridae family. Transmission electron microscopy revealed that IME278 had an icosahedral head 51.72 nm in diameter and a tail 151.28 nm in length. Bacteriophage IME278 was able to survive under high temperature (50 °C–70 °C) and its activity decreased significantly above 70 °C and almost completely inactivated at 80 °C. Bacteriophage IME278 could survive in a wide pH range (4.0–11.0) and it was stable in chloroform (up to 5%). The phage was sensitive to UV irradiation. Bacteriophage IME278 had a latent period of 40 min and reached a plateau stage at 150 min and its cleavage was approximately 8.21 × 108/3.66 × 108 = 2.24. The biocontrol potential of bacteriophage IME278 was evaluated in a model that artificially contaminated pork with E. hormaechei 529 and the result revealed that IME278 could effectively control bacterial contamination on pork. The in-depth analysis of the biological characteristics, whole genome sequencing, and bioinformatics of IME278 has laid the foundation for the biocontrol application and the treatment of bacteria using bacteriophages. • The discovery of E. hormaechei bacteriophage IME278 expand our knowledge of the diversity of phage contingent. • E. hormaechei bacteriophage IME278 shared 87% homology with other phages, indicating IME278 can be classified as a new genus. • E. hormaechei bacteriophage IME278 was similar to bacteriophages belonging to the genus Kayfunavirus , family Autographiviridae. • The biocontrol potential of bacteriophage IME278 revealed that IME278 could effectively control bacterial contamination on pork. [ABSTRACT FROM AUTHOR]

• A newly isolated phages (IME184) can specifically infect the Klebsiella pneumoniae. • Phage genome composed of 44,598 bp with a GC content of 50.3%. • IME is classified as an Autographiviridae bacteriophage. Klebsiella pneumoniae, a multidrug resistant bacterium that causes nosocomial infections including septicemia, pneumonia etc. Bacteriophages are potential antimicrobial agents for the treatment of antibiotic resistant bacteria. In this study, a novel bacteriophage IME184, was isolated from hospital sewage against clinical multi-drug resistant Klebsiella pneumoniae. Transmission electron microscopy and genomic characterization exhibited this phage belongs to the Molineuxvirinae genus, Autographiviridae family. Phage IME184 possessed a double-stranded DNA genome composed of 44,598 bp with a GC content of 50.3%. The phage genome encodes 57 open reading frames, out of 26 are hypothetical proteins while 31 had assigned putative functions. No tRNA, virulence related or antibiotic resistance genes were found in phage genome. Comparative genomic analysis showed that phage IME184 has 94% similarity with genomic sequence of Klebsiella phage K1-ULIP33 (MK380014.1). Multiplicity of infection, one step growth curve and host range of phage were also measured. According to findings, Phage IME184 is a promising biological agent that infects Klebsiella pneumoniae and can be used in future phage therapies. [ABSTRACT FROM AUTHOR]