3 results on '"Tue, Ngo Tri"'
Search Results
2. Unbiased whole-genome deep sequencing of human and porcine stool samples reveals circulation of multiple groups of rotaviruses and a putative zoonotic infection
- Author
-
Juliet E. Bryant, Maia A. Rabaa, Phuc Tran My, Guy E. Thwaites, Paul Kellam, Nguyen Van Cuong, Mark E. J. Woolhouse, My V. T. Phan, Tue Ngo Tri, Lia van der Hoek, Bas B. Oude Munnink, Pham Hong Anh, Stephen Baker, Matthew Cotten, Wellcome Trust, Medical Microbiology and Infection Prevention, and Amsterdam institute for Infection and Immunity
- Subjects
VIETNAM ,0301 basic medicine ,virus surveillance ,UNITED-STATES ,VACCINE ,CHILDREN ,Genomics ,zoonotic infection ,Biology ,medicine.disease_cause ,Microbiology ,Group A ,Genome ,Deep sequencing ,Virus ,03 medical and health sciences ,deep sequencing ,whole genomes ,ACUTE GASTROENTERITIS ,Virology ,Rotavirus ,Genotype ,medicine ,on behalf of the VIZIONS Consortium ,030304 developmental biology ,Genetics ,0303 health sciences ,Science & Technology ,IDENTIFICATION ,Phylogenetic tree ,Zoonotic Infection ,030306 microbiology ,STRAINS ,BOVINE ROTAVIRUS ,DIARRHEA ,3. Good health ,030104 developmental biology ,rotavirus ,VIZIONS Consortium ,GROUP-A ROTAVIRUSES ,Life Sciences & Biomedicine ,Research Article - Abstract
Coordinated and synchronous virological surveillance for zoonotic viruses in both human clinical cases and animal reservoirs provides an opportunity to identify interspecies virus movement. Rotavirus is an important cause of viral gastroenteritis in humans and animals. We have documented the rotavirus diversity within co-located humans and animals sampled from the Mekong delta region of Vietnam using a primer-independent, agnostic, deep sequencing approach. A total of 296 stool samples (146 from diarrhoeal human patients and 150 from pigs living in the same geographical region) were directly sequenced, generating the genomic sequences of 60 human rotaviruses (all group A) and 31 porcine rotaviruses (13 group A, 7 group B, 6 group C and 5 group H). Phylogenetic analyses showed the co-circulation of multiple distinct rotavirus group A (RVA) genotypes/strains, many of which were divergent from the strain components of licensed RVA vaccines, as well as considerable virus diversity in pigs including full genomes of rotaviruses in groups B, C and H, none of which have been previously reported in Vietnam. Furthermore the detection of an atypical RVA genotype constellation (G4-P[6]-I1-R1-C1-M1-A8-N1-T7-E1-H1) in a human patient and a pig from the same region provides some evidence for a zoonotic event
- Published
- 2016
3. New Variant of Multidrug-Resistant Salmonella enterica Serovar Typhimurium Associated with Invasive Disease in Immunocompromised Patients in Vietnam
- Author
-
Mather, Alison E, Phuong, Tu Le Thi, Gao, Yunfeng, Clare, Simon, Mukhopadhyay, Subhankar, Goulding, David A, Hoang, Nhu Tran Do, Tuyen, Ha Thanh, Lan, Nguyen Phu Huong, Thompson, Corinne N, Trang, Nguyen Hoang Thu, Carrique-Mas, Juan, Tue, Ngo Tri, Campbell, James I, Rabaa, Maia A, Thanh, Duy Pham, Harcourt, Katherine, Hoa, Ngo Thi, Trung, Nguyen Vinh, Schultsz, Constance, Perron, Gabriel G, Coia, John E, Brown, Derek J, Okoro, Chinyere, Parkhill, Julian, Thomson, Nicholas R, Chau, Nguyen Van Vinh, Thwaites, Guy E, Maskell, Duncan J, Dougan, Gordon, Kenney, Linda J, and Baker, Stephen
- Subjects
Salmonella typhimurium ,Genotype ,Swine ,Bacteremia ,HIV Infections ,Immunocompromised Host ,Drug Resistance, Multiple, Bacterial ,Zoonoses ,genomics ,Disease Transmission, Infectious ,Animals ,Humans ,antimicrobial resistance ,Molecular Epidemiology ,Salmonella Infections, Animal ,Whole Genome Sequencing ,Genetic Variation ,3. Good health ,invasive salmonellosis ,Gastroenteritis ,Ducks ,Vietnam ,Carrier State ,Salmonella Infections ,bacteria ,Chickens - Abstract
Nontyphoidal Salmonella (NTS), particularly Salmonella enterica serovar Typhimurium, is among the leading etiologic agents of bacterial enterocolitis globally and a well-characterized cause of invasive disease (iNTS) in sub-Saharan Africa. In contrast, S Typhimurium is poorly defined in Southeast Asia, a known hot spot for zoonotic disease with a recently described burden of iNTS disease. Here, we aimed to add insight into the epidemiology and potential impact of zoonotic transfer and antimicrobial resistance (AMR) in S Typhimurium associated with iNTS and enterocolitis in Vietnam. We performed whole-genome sequencing and phylogenetic reconstruction on 85 human (enterocolitis, carriage, and iNTS) and 113 animal S Typhimurium isolates isolated in Vietnam. We found limited evidence for the zoonotic transmission of S Typhimurium. However, we describe a chain of events where a pandemic monophasic variant of S Typhimurium (serovar I:4,[5],12:i:- sequence type 34 [ST34]) has been introduced into Vietnam, reacquired a phase 2 flagellum, and acquired an IncHI2 multidrug-resistant plasmid. Notably, these novel biphasic ST34 S Typhimurium variants were significantly associated with iNTS in Vietnamese HIV-infected patients. Our study represents the first characterization of novel iNTS organisms isolated outside sub-Saharan Africa and outlines a new pathway for the emergence of alternative Salmonella variants into susceptible human populations.IMPORTANCESalmonella Typhimurium is a major diarrheal pathogen and associated with invasive nontyphoid Salmonella (iNTS) disease in vulnerable populations. We present the first characterization of iNTS organisms in Southeast Asia and describe a different evolutionary trajectory from that of organisms causing iNTS in sub-Saharan Africa. In Vietnam, the globally distributed monophasic variant of Salmonella Typhimurium, the serovar I:4,[5],12:i:- ST34 clone, has reacquired a phase 2 flagellum and gained a multidrug-resistant plasmid to become associated with iNTS disease in HIV-infected patients. We document distinct communities of S Typhimurium and I:4,[5],12:i:- in animals and humans in Vietnam, despite the greater mixing of these host populations here. These data highlight the importance of whole-genome sequencing surveillance in a One Health context in understanding the evolution and spread of resistant bacterial infections.
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.